Biophysical Comparison of Two Canine Adenoviruses

Marusyk, R. G.; Norrby, Erling; Lundqvist, Ulla

doi:10.1128/jvi.5.4.507-512.1970

Cited by 29 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The discovered strain was named Toronto A26/61 and it was initially regarded as a variety of CAV-1. However, further studies showed structural and antigen differences between these viral isolates and as a result Toronto A26/61 was recognised as a separate strain and named CAV-2 (Yamamoto and Marusyk 1968, Fairchild and Cohen 1969, Swango et al 1969, Marusyk et al 1970, Marusyk 1972, Hamelin et al 1984. The total analysis of the genome of these adenoviruses showed their homology only in 75% (Morrison et al 1997, Davison et al 2003.…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough

Kalinowski

Adaszek²,

Miłoszowska³

et al. 2012

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study was to perform molecular analysis of canine adenovirus 2 (CAV-2) E1B 19K gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the Lubelszczyzna region. Nasal swabs were taken from dogs. DNA of CAV-2 was detected using the PCR method in 16 swabs. All PCR products were sequenced, and the obtained sequences were compared with each other and with the sequence of the E1B 19K gene of the CAV-2 strain from an online database of NCBI GenBank: AC 000003. Based on analysis of the obtained sequences, three polymorphic variants of CAV-2 (No.1-3) with homology of 78 - 100% were distinguished. The nucleotide and amino acid sequences of the most frequently represented polymorphic variant, No. 1, differed from the sequences of polymorphic variant No. 2 with one substitution. The nucleotide and amino acid sequence of the E1B 19K gene of CAV-2 AC 000003 differed from the analogous sequences of representatives of variant No. 1 with 44 nucleotide and 19 amino acid substitutions. The small number of nucleotide differences in the E1B 19K CAV-2 gene among the examined own isolates, compared with AC 000003, suggest that the infections in dogs were caused by a relatively genetically stable virus which occurs in eastern Poland.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough

Kalinowski

Adaszek²,

Miłoszowska³

et al. 2012

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…Virus purification. ICL adenovirus was purified from the infected cells by centrifuging twice in CsCl (14).…”

mentioning

confidence: 99%

Macromolecular Content of Inclusions Produced by a Canine Adenovirus

Shahrabadi

Roy

Yamamoto

1972

J Virol

View full text Add to dashboard Cite

Early inclusions induced by a canine adenovirus in a canine cell line, appearing before the formation of infectious virus particles, were purified by differential centrifugation in sucrose followed by CsCl density gradient centrifugation. Chemical analysis of these inclusions revealed that they contained deoxyribonucleic acid (DNA), ribonucleic acid, and protein. On the basis of density gradient centrifugation, the DNA extracted from the inclusions was found to be viral DNA. Electron microscope autoradiography showed that these inclusions were the sites of DNA synthesis. In addition, association of DNA polymerase activity with the inclusions was detected by incorporation of radioactivity from 3H-thymidine triphosphate into a DNA product. The in vitro product of the enzyme had a density equal to that of viral DNA rather than host DNA. The level of DNA polymerase activity in exponentially growing infected and uninfected whole cells was similar, but in cells in stationary phase the enzyme activity of infected cells was twice that in noninfected cells. Furthermore, nuclei isolated from infected cells showed a fourfold increase in DNA polymerase activity over the noninfected cells. After adenovirus infection, various kinds of intranuclear inclusions appear at different times. These inclusions have been studied extensively with the light and electron microscopes (3, 6, 13, 15). Reports with regard to the nature of these inclusions have been based on cytochemical and autoradiographic studies (12, 13, 15). Similar sequential studies of the inclusions have also been reported for a canine adenovirus (24). These inclusions were (i) the early granular inclusions and ring-form bodies which appeared before the formation of infectious virus particles and (ii) the dark-staining and light-staining inclusions which were formed after the appearance of virus particles. Histochemical and autoradiographic studies (27) showed that the early granular inclusions and the ring-form bodies were similar in their composition, both types being composed of deoxyribonucleic acid (DNA), protein, and ribonucleic acid (RNA). On the basis of morphological and cytological studies, it was concluded that the ring-form bodies developed from the early granular inclusions. The present study further elucidates the nature of the early granular and ring-form inclusions and their role in virus multiplication as determined by purification of these inclusions and characterization of them with regard to their macromolecular content. MATERIALS AND METHODS Virus and virus assay. The strain of infectious canine laryngotracheitis (ICL) virus was previously characterized (25, 26) and was propagated and assayed as described (27). Tissue culture and infection of cells. The Madin-Darby canine kidney (MDCK) cell line (CCL-34), obtained from American Type Culture Collection Cell Repository, Rockville, Md., was grown in monolayer at 37 C in Roux bottles. Growth medium was Eagle minimal essential medium supplemented with 5%, calf serum penicillin (100 units/ml) and streptom...

show abstract

“…It was found to be antigenically related to CAV-1; however, the tissue tropism of both viruses is entirely different (Appel et al, 1973). The virus was later classified as CAV-2 (Hamelin et al, 1984;Marusyk et al, 1970). The attenuated CAV-2 proved to protect dogs against infection with both CAV-1 and CAV-2 (Appel et al, 1975).…”

Section: Canine Adenovirus Typementioning

confidence: 99%

Forty Years of Canine Vaccination

Appel¹

1999

Advances in Veterinary Medicine

View full text Add to dashboard Cite

Biophysical Comparison of Two Canine Adenoviruses

Cited by 29 publications

References 15 publications

Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough

Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough

Macromolecular Content of Inclusions Produced by a Canine Adenovirus

Forty Years of Canine Vaccination

Contact Info

Product

Resources

About