Biophysical Comparisons of Calicivirus Serotypes Isolated from Pinnipeds

Soergel, M. E.; Smith, Alvin W.; Schaffer, Frederick L.

doi:10.1159/000149920

Cited by 11 publications

(7 citation statements)

References 5 publications

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“…Techniques for rate zonal cen~rifugation in glycerol gradients (29), and isopycnic centrifugation in CsCl (38) were similar to those previously described, except tha~ Ca ++ and Mg ++ were included in the phosphate buffered saline (PBS) since FCV and CaCV showed evidence of aggregation in absence of these divalent cations. For collecting fractio~ from the bottem of the tube, the device described by HECXLY (15) was modified using oil displacement from a syringe pump.…”

Section: Physico-chemical Characterizationmentioning

confidence: 99%

Characterization of a new calicivirus isolated from feces of a dog

Schaffer¹,

Soergel²,

Black³

et al. 1985

Archives of Virology

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Canine calicivirus (CaCV), isolated from feces of a dog with diarrhea, was readily propagated in cultures of canine cells and in a dolphin cell line. Serologic evidence indicated many dogs in at least one geographic area had been infected with CaCV, but its role as an etiologic agent of disease was not established. In cell culture most CaCV virions were strongly cell-associated making purification difficult. CaCV was established as a member of the Caliciviridae by morphology and physicochemical properties of virions (density, sedimentation rate, single major polypeptide, RNA genome size), although some of the properties differed slightly from those of previously described caliciviruses; evidence was also obtained for caliciviral RNA species in infected cells. Based on tests with antisera to numerous caliciviruses and presumed caliciviruses, CaCV appeared to be not closely related to any previously described virus except the stunting syndrome agent of chickens.

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Section: Physico-chemical Characterizationmentioning

confidence: 99%

Characterization of a new calicivirus isolated from feces of a dog

Schaffer¹,

Soergel²,

Black³

et al. 1985

Archives of Virology

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“…The viruses used as preeipitin antigens were SMSV-4 and SMSV-5 (16,18). A preparation of SMSV-4 was purified, cross-linked with dimethyl suberimidate to preserve virion integrity, and labeled with 125I essentially as described (13).…”

Section: Virus Antigenmentioning

confidence: 99%

Assay of antibodies to caliciviruses by radioimmune precipitation using staphylococcal protein A as IgG adsorbent

Soergel¹,

Schaffer²,

Sawyer

et al. 1978

Archives of Virology

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A radioimmune assay method designated St-RIP using a staphylococcal IgG adsorbent, which potentially has broad applications to viral (and nonviral) antigen-antibody systems, was applied to detection of calicivirus antibodies. Purified 125I-labeled virions of San Miguel sea lion virus serotypes 4 (SMSV-4) and 5 (SMSV 5) were incubated with sera; the immune complexes were reacted with an immunoadsorbent, formaldehyde-fixed staphylococci (Staphylococcus aureus protein A producer, strain Cowan I), and collected by centrifugation. Broad cross-reactivity was observed among serotypes of SMSV and vesicular exanthema of swine virus (VESV), but there was no reaction with antisera to six noncaliciviruses. Antibody production in a rabbit inoculated with SMSV-5 polypeptide was monitored by St-RIP assay; reactivity with intact SMSV-4 virion antigen was slightly less than, but closely paralleled, reactivity with SMSV-5 virion antigen. Applicability of the St-RIP test to serologic survey was demonstrated with pinniped, swine, and human (laboratory personnel) sera; numerous positive St-RIP reactions suggested the occurrence of widespread contacts with caliciviruses.

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“…Type 2 SMSV, type A,, VESV, and strain F-9 of FCV were propagated in Vero, porcine kidney (PK15), and Crandall feline kidney (FK) cells, respectively. General methodology was as previously described (11,(13)(14)(15). For intracellular viral RNA, cells in 3-ounce (ca.…”

mentioning

confidence: 99%

RNA synthesized in calicivirus-infected cells is atypical of picornaviruses

Ehresmann¹,

Schaffer²

1977

J Virol

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RNA labeled with [3H]uridine from Vero cells infected with San Miguel sea lion virus in the presence of actinomycin D was analyzed by glycerol density gradient sedimentation and polyacrylamide gel electrophoresis. The predominant single-stranded RNA (36S, 2.6 x 106 molecular weight) was genome size. There was also a prominent 22S, 1.1 x 106-molecular weight, single-stranded component and one or more double-stranded or partially double-stranded classes. Replicative forms, sedimenting at 1&S, contained single-stranded RNA corresponding to the larger-molecular-weight class. All classes of intracellular RNA and virion RNA were polyadenylated. These findings and results with pig kidney cells infected with vesicular exanthema of swine virus and feline cells infected with feline calicivirus indicate that caliciviruses exhibit a strategy of replication different from typical picornaviruses and supports removal of the caliciviruses from the family Picornaviridae.

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Biophysical Comparisons of Calicivirus Serotypes Isolated from Pinnipeds

Cited by 11 publications

References 5 publications

Characterization of a new calicivirus isolated from feces of a dog

Characterization of a new calicivirus isolated from feces of a dog

Assay of antibodies to caliciviruses by radioimmune precipitation using staphylococcal protein A as IgG adsorbent

RNA synthesized in calicivirus-infected cells is atypical of picornaviruses

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