Biophysical Mechanism of Parasympathetic Excitation of Urinary Bladder Smooth Muscle Cells: a Simulation Study

A biophysically constrained computational model of the action potential of mouse urinary bladder smooth muscle

“…A computational model for detrusor smooth muscle spikes has previously been reported [ 37 ]. In contrast to our model, which is biophysically explicit, the previous model is an implicit one.…”

“…To the best of our knowledge, active voltage-gated Na + and Ca 2+ dependent Cl - channels have not been documented for DSM cells of any species, yet the prior model has incorporated these channels in order to simulate the action potential. Conversely, while experimental studies provide substantial evidence for the presence in DSM cells of intermediate conductance Ca 2+ -activated K + channels, ATP-dependent K + channels and inwardly-rectifying channels, these channels are not included in the model of Korogod et al, 2014 [ 37 ]. In endeavoring to build a physiologically more realistic model, constrained by available data, we have incorporated the latter channels but not the former.…”

“…By contrast, computational models for DSM cells are at a relatively nascent stage. Although an electrophysiological model of DSM cell based on the Hodgkin-Huxley formalism was presented recently [ 37 ], detailed descriptions of the biophysical characteristics of each of the DSM ionic currents are still lacking. One of the key conflicts between the action potential of the recently published model and the known DSM electrophysiology is that the former is based on an active sodium conductance, whereas most experimental studies do not indicate the presence of voltage-gated sodium ion channels in DSM [ 23 , 38 ]; other salient conflicts also exist (see Discussion ).…”