Biophysical Study of the Perturbation of Model Membrane Structure Caused by Seminal Plasma Protein PDC-109

Gasset, Marı́a; Magdaleno, Leticia; Calvete, Juan J.

doi:10.1006/abbi.1999.1593

Cited by 49 publications

(65 citation statements)

References 25 publications

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“…In another study, interaction of PDC-109 protein with artificial phosphorylcholine vesicles provoked permeability changes and partial disruption of the vesicles (Gasset et al, 2000;Damai et al, 2010). Studies revealed similar cholesterol efflux mediated by BSP proteins (120 g/mL) or 0.25-0.5% of whole seminal plasma (containing approximately the same amount of BSP proteins, Therien et al, 1998).…”

Section: Cholesterol Efflux From Sperm Membranementioning

confidence: 93%

Bovine seminal PDC-109 protein: An overview of biochemical and functional properties

Srivastava

Adnot

Srivastava

et al. 2013

Animal Reproduction Science

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Section: Cholesterol Efflux From Sperm Membranementioning

confidence: 93%

Bovine seminal PDC-109 protein: An overview of biochemical and functional properties

Srivastava

Adnot

Srivastava

et al. 2013

Animal Reproduction Science

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“…Careful control experiments are needed to compensate for bulk effects such as the heat of dilution of the ligand and the receptor, and the heat of mixing [102,103]. ITC has been routinely used to study many types of binding reactions [101,103,104,105,106] including, protein-protein [107], protein-membrane [108,109,110,111], and drug-receptor [106,112] interactions.…”

Section: Calorimetrymentioning

confidence: 99%

Label-free screening of bio-molecular interactions

Cooper

2003

Analytical and Bioanalytical Chemistry

416

297

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The majority of techniques currently employed to interrogate a biomolecular interaction require some type of radio- or enzymatic- or fluorescent-labelling to report the binding event. However, there is an increasing awareness of novel techniques that do not require labelling of the ligand or the receptor, and that allow virtually any complex to be screened with minimal assay development. This review focuses on three major label-free screening platforms: surface plasmon resonance biosensors, acoustic biosensors, and calorimetric biosensors. Scientists in both academia and industry are using biosensors in areas that encompass almost all areas drug discovery, diagnostics, and the life sciences. The capabilities and advantages of each technique are compared and key applications involving small molecules, proteins, oligonucleotides, bacteriophage, viruses, bacteria, and cells are reviewed. The role of the interface between the biosensor surface (in the case of SPR and acoustic biosensors) and the chemical or biological systems to be studied is also covered with attention to the covalent and non-covalent coupling chemistries commonly employed.

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“…Ca 2+ influx into sperm [38] and subsequent sperm capacitation [12] in the bull are induced by heparin bound to glycoproteins on the surface of the plasma membrane of the ejaculated sperm. The results of the present study indicate that there are heparin-binding glycoproteins on the plasma membrane of the entire heads and mid-pieces of canine ejaculated sperm.…”

Section: Discussionmentioning

confidence: 99%

Effects of Hyaluronic Acid on Ca<sup>2+</sup> Influx, Lactate Dehydrogenase Activity, and Cyclic AMP Synthesis in Canine Ejaculated Sperm during <i>In Vitro</i> Capacitation

Kawakami

Nishizono

Miyata

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. The effects of hyaluronic acid, which comprises the cumulus intercellular matrix, on Ca 2+ influx, lactate dehydrogenase (LDH) activity, and cyclic AMP synthesis in canine sperm during capacitation was investigated. Ejaculated sperm were collected from 10 Beagle dogs and the sperm were incubated for 4 hr in Eagle's MEM containing 10 µg/ml of hyaluronic acid. The percentages of actively motile sperm, hyperactivated sperm (HA-sperm), acrosome-reacted sperm (AR-sperm), and sperm labeled with fluoresceinated Ca 2+ indicator (Ca 2+ -labeled sperm) were evaluated to assess Ca 2+ influx into the sperm. LDH activity and cAMP concentration were measured in homogenized sperm. The mean percentages of motile sperm, HA-sperm, and Ca 2+ -labeled sperm in the MEM containing hyaluronic acid were higher than in the control medium (P<0.05, 0.05, and 0.01, respectively), but there was no difference between the percentages of AR-sperm. Mean LDH activity and mean cAMP concentration were also significantly higher than the control values (P<0.05). Sperm capacitation consists of changes in movement in the form of hyperactivation (HA) and in morphology in the form of acrosome reaction (AR), and HA and AR are necessary for canine sperm to be able to penetrate oocytes [28,29]. The oviductal fluids of the estrous cow [2,13,25,39] and bitch [19] have been reported to contain a large amount of glycosaminoglycans (GAGs). GAGs are mucopolysaccharides, which includes hyaluronic acid and heparin, that induce sperm capacitation in the oviducts [27,31]. The cumulus intercellular matrix of oocytes is comprised of hyaluronic acid in many species [3,33,43] It is well known that sperm capacitation is induced by Ca 2+ influx into the cytoplasm of sperm [11,41]. Energy metabolism of sperm is closely related to the activity of lactate dehydrogenase (LDH) enzyme in the sperm [6,7,17,24,35,41], and high LDH activity is maintained during sperm capacitation [35]. The flagellar movement of the canine sperm tail has been reported to be induced by an increase in cyclic adenosine monophosphate (cAMP) concentration in the sperm [47]. In other species [1,15,16], energy for sperm movement is produced by an increase in the cAMP concentration in the sperm as well as an increase in LDH activity. It is thought that cAMP production is stimulated by an increase of Ca 2+ concentration in the sperm cytoplasm [14,41] and that sperm capacitation is subsequently induced [40].In the present study, canine ejaculated sperm were incubated in culture medium supplemented with hyaluronic acid to assess the effect of hyaluronic acid on Ca 2+ influx, LDH activity, and cAMP concentration in the sperm. The binding site of GAGs containing hyaluronic acid on the ejaculated sperm surface was investigated using fluorescein isothiocyanate (FITC)-labeled heparin.

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Biophysical Study of the Perturbation of Model Membrane Structure Caused by Seminal Plasma Protein PDC-109

Cited by 49 publications

References 25 publications

Bovine seminal PDC-109 protein: An overview of biochemical and functional properties

Bovine seminal PDC-109 protein: An overview of biochemical and functional properties

Label-free screening of bio-molecular interactions

Effects of Hyaluronic Acid on Ca<sup>2+</sup> Influx, Lactate Dehydrogenase Activity, and Cyclic AMP Synthesis in Canine Ejaculated Sperm during <i>In Vitro</i> Capacitation

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