Biophysics of Membrane Stiffening by Cholesterol and Phosphatidylinositol 4,5-bisphosphate (PIP2)

Doole, Fathima T.; Gupta, Sudipta; Kumarage, Teshani; Ashkar, Rana; Brown, Michael F.

doi:10.1007/978-3-031-21547-6_2

Cited by 5 publications

(2 citation statements)

References 122 publications

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“…Taking into account previous lipidomic analyses [ 6 , 7 , 9 ] as well, the simplest explanation for these data is indeed an increase in the (local) concentration of cholesterol and/or saturated lipids (including, e.g., sphingolipids). Of interest, this interpretation is compatible with our results regarding the possible increase of negatively charged lipids: on one hand, an increase in cholesterol can be accompanied by higher levels of the anionic lipid PIP2 in cellular membranes (reviewed in [ 84 ]); on the other hand, a recent report linked the presence of PS to enhanced interleaflet coupling in model membranes and, as a consequence, an increase in membrane stiffness [ 85 ]. Furthermore, an increase in PM cholesterol concentration might at least partially explain the enhanced M2 clustering (see Figure S2 , and compared to transfected cells [ 46 ]) which is, in fact, modulated by cholesterol [ 35 , 39 , 40 ].…”

Section: Discussionsupporting

confidence: 90%

Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane

Petrich,

Chiantia

2023

Viruses

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The pathogenesis of influenza A viruses (IAVs) is influenced by several factors, including IAV strain origin and reassortment, tissue tropism and host type. While such factors were mostly investigated in the context of virus entry, fusion and replication, little is known about the viral-induced changes to the host lipid membranes which might be relevant in the context of virion assembly. In this work, we applied several biophysical fluorescence microscope techniques (i.e., Förster energy resonance transfer, generalized polarization imaging and scanning fluorescence correlation spectroscopy) to quantify the effect of infection by two IAV strains of different origin on the plasma membrane (PM) of avian and human cell lines. We found that IAV infection affects the membrane charge of the inner leaflet of the PM. Moreover, we showed that IAV infection impacts lipid–lipid interactions by decreasing membrane fluidity and increasing lipid packing. Because of such alterations, diffusive dynamics of membrane-associated proteins are hindered. Taken together, our results indicate that the infection of avian and human cell lines with IAV strains of different origins had similar effects on the biophysical properties of the PM.

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Section: Discussionsupporting

confidence: 90%

Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane

Petrich,

Chiantia

2023

Viruses

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“…These findings indicate the need to reassess the role of cholesterol in governing membrane bending stiffness, particularly at intermediate length and time scales relevant to essential biological functions such as virus budding and lipid-protein interactions. In 2023, Doole et al utilized solid-state deuterium nuclear magnetic resonance ( 2 H NMR) and NSE spectroscopy to reveal a synergistic modulation of lipid packing by cholesterol and PIP2, resulting in alterations in the bending stiffness of lipid membranes [101].…”

Section: Lipid Membranesmentioning

confidence: 99%

Applications of neutron spin echo in soft matter

Luo,

Cui,

Chu

2023

Front. Phys.

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Soft matter systems exhibit diversity and intricacy in their structures and properties, with their dynamic behaviors and structural changes spanning wide time and length scales. Gaining insight into the internal structures and dynamics behaviors of soft matter systems, as well as the interactions among molecules and particles, contributes to a deeper comprehension of the microscopic behaviors of matter. Moreover, this endeavor has significant biomedical and materials engineering implications. This review focuses on the applications of spin-echo small-angle neutron scattering (SESANS) and high-resolution neutron spin echo (NSE) spectroscopy in soft matter science, particularly complex fluids and biomolecular systems. NSE spectroscopy has remarkable temporal resolution and sensitivity towards molecular-scale dynamic behaviors. Therefore, it provides comprehensive insights into microscale dynamic phenomena to soft matter systems, such as the rheological behaviors, stability, and aggregation dynamics of colloids; the domain dynamics and conformational changes of proteins; the collective dynamics of lipid membranes and interactions with other molecules, as well as the dynamic behaviors and interactions of surfactants within microemulsions. NSE technique helps reveal the complex nature of these systems, providing valuable insights into advances in materials science, biomedicine, and chemistry.

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Solid-state NMR spectroscopy

Aliev

2024

Nuclear Magnetic Resonance

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This solid-state NMR report summarises the subject and results of selected articles and original papers published in 2023. In addition to the published reviews, the two major sections in this report consider various methodological developments and a broad range of applications. The presented classification of papers into different sections and subsections is intended to ease the overview of the results, as well as to evaluate the directions in which solid-state NMR techniques and applications are likely to develop in the near future. The overall survey of published papers indicates that the number of electrolyte and battery materials studies by solid-state NMR has increased considerably in 2023 compared to previous years.

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Biophysics of Membrane Stiffening by Cholesterol and Phosphatidylinositol 4,5-bisphosphate (PIP2)

Cited by 5 publications

References 122 publications

Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane

Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane

Applications of neutron spin echo in soft matter

Solid-state NMR spectroscopy

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