Bioplasmonic Paper as a Platform for Detection of Kidney Cancer Biomarkers

Tian, Limei; Morrissey, Jeremiah J.; Kattumenu, Ramesh; Gandra, Naveen; Kharasch, Evan D.; Singamaneni, Srikanth

doi:10.1021/ac302332g

Cited by 90 publications

(74 citation statements)

References 47 publications

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“…As described earlier, conjugation of AuNRs with the antibodies follows two steps. 50 The IgG molecules were initially modified with a bifunctional polyethylene glycol chain (COOH-PEG-SH) using a carbodiimide coupling chemistry. IgG-PEG-SH was then covalently attached onto the AuNRs surface through a gold-thiol linkage.…”

Section: Resultsmentioning

confidence: 99%

Ultrarobust Biochips with Metal–Organic Framework Coating for Point-of-Care Diagnosis

Wang

Tadepalli

et al. 2018

ACS Sens.

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Most biosensors relying on antibodies as recognition elements fail in harsh environment conditions such as elevated temperatures, organic solvents or proteases because of antibody denaturation, and require strict storage conditions with defined shelf-life, thus limiting their applications in point-of-care and resource-limited settings. Here, a metal-organic framework (MOF) encapsulation is utilized to preserve the biofunctionality of antibodies conjugated to nanotransducers. This study investigates several parameters of MOF coating (including growth time, surface morphology, thickness and precursor concentrations) that determine the preservation efficacy against different protein denaturing conditions in both dry and wet environments. A plasmonic biosensor based on gold nanorods as the nanotransducers is employed as a model biodiagnostic platform. The preservation efficacy attained through MOF encapsulation is compared to two other commonly employed materials (sucrose and silk fibroin). The results show that MOF coating outperforms sucrose and silk fibroin coatings under several harsh conditions including high temperature (80 °C), dimethylformamide and protease solution, owing to complete encapsulation, stability in wet environment and ease of removal at point-of-use by the MOF. We believe this study will broaden the applicability of this universal approach for preserving different types of on-chip biodiagnostic reagents and biosensors/bioassays, thus extending the benefits of advanced diagnostic technologies in resource-limited settings.

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Section: Resultsmentioning

confidence: 99%

Ultrarobust Biochips with Metal–Organic Framework Coating for Point-of-Care Diagnosis

Wang

Tadepalli

et al. 2018

ACS Sens.

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“…Additionally, fluids can wick through (hydrophilic) cellulose through capillary actions, thereby eliminating the necessity for using external fluid pumps. As a result, lPADs have emerged as a promising platform for point-of-care analytical applications in clinical diagnostics, [1][2][3] food safety control, 4,5 and environmental testing. 6,7 Various techniques have been used to fabricate lPADs, including photolithography, 8,9 wax printing, [10][11][12] inkjet printing, [13][14][15] laser etching, 16,17 plasma treatment, 18 and use of metal/paper masks.…”

Section: Introductionmentioning

confidence: 99%

Defining microchannels and valves on a hydrophobic paper by low-cost inkjet printing of aqueous or weak organic solutions

Cai

Zhong

et al. 2015

Biomicrofluidics

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We describe a simple and cost-effective strategy for rapid fabrication of microfluidic paper-based analytical devices and valves by inkjet printing. NaOH aqueous solution was printed onto a hydrophobic filter paper, which was previously obtained by soaking in a trimethoxyoctadecylsilane-heptane solution, allowing selective wet etching of hydrophobic cellulose to create hydrophilic-hydrophobic contrast with a relatively good resolution. Hexadecyltrimethylammonium bromide (CTMAB)-ethanol solution was printed onto hydrophobic paper to fabricate temperaturecontrolled valves. At low temperature, CTMAB deposited on the paper is insoluble in aqueous fluid, thus the paper remains hydrophobic. At high temperature, CTMAB becomes soluble so the CTMAB-deposited channel becomes hydrophilic, allowing the wicking of aqueous solution through the valve. We believe that this strategy will be very attractive for the development of simple micro analytical devices for point-of-care applications, including diagnostic testing, food safety control, and environmental monitoring. V C 2015 AIP Publishing LLC.

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“…Owing to numerous advantages such as high specific surface area, excellent wicking properties, compatibility with conventional printing approaches, significant cost reduction and easy disposability, paper substrates are gaining increased attention in biodiagnostics, food quality testing, environmental monitoring, flexible energy and electronic devices (Chen et al, 2008; Cheng et al, 2010; Huang et al, 2013; Lee et al, 2010, 2011; Li et al, 2010, 2012; Martinez et al, 2007, 2009; Nergiz et al, 2013; Parolo and Merkoci, 2013; Tian et al, 2012c). Recent surge in the activity related to paper-based diagnostic devices is primarily focused on realizing microfluidic paper-based analytical devices (µPADs) for point-of-care assays and inexpensive diagnostic tools for resource-limited environments (Lewis et al, 2012; Martinez et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…We have recently demonstrated plasmonic paper comprised of biofunctionalized gold nanorods (AuNRs) uniformly adsorbed on paper substrates (Tian et al, 2012c). The bioplasmonic paper enabled the detection of aquaporin-1, a kidney cancer biomarker in artificial urine down to a concentration of 10 ng/ml (Morrissey et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Bioplasmonic calligraphy for multiplexed label-free biodetection

Tian

Tadepalli

Park

et al. 2014

Biosensors and Bioelectronics

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Printable multi-marker biochips that enable simultaneous quantitative detection of multiple target biomarkers in point-of-care and resource-limited settings are a holy grail in the field of biodiagnostics. However, preserving the functionality of biomolecules, which are routinely employed as recognition elements, during conventional printing approaches remains challenging. In this article, we introduce a simple yet powerful approach, namely plasmonic calligraphy, for realizing multiplexed label-free bioassays. Plasmonic calligraphy involves a regular ballpoint pen filled with biofunctionalized gold nanorods as plasmonic ink for creating isolated test domains on paper substrates. Biofriendly plasmonic calligraphy approach serves as a facile method to miniaturize the test domain size to few mm2, which significantly improves the sensitivity of the plasmonic biosensor compared to bioplasmonic paper fabricated using immersion approach. Furthermore, plasmonic calligraphy also serves as a simple and efficient means to isolate multiple test domains on a single test strip, which facilitates multiplexed biodetection and multi-marker biochips. Plasmonic calligraphy, which can be potentially automated by implementing with a robotic arm, serves as an alternate path forward to overcome the limitations of conventional ink-jet printing.

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Bioplasmonic Paper as a Platform for Detection of Kidney Cancer Biomarkers

Cited by 90 publications

References 47 publications

Ultrarobust Biochips with Metal–Organic Framework Coating for Point-of-Care Diagnosis

Ultrarobust Biochips with Metal–Organic Framework Coating for Point-of-Care Diagnosis

Defining microchannels and valves on a hydrophobic paper by low-cost inkjet printing of aqueous or weak organic solutions

Bioplasmonic calligraphy for multiplexed label-free biodetection

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