Biopolymer-based intra-articular delivery of DKK1 into the contracted rabbit knee

Jo, Suenghwan; Lewallen, Eric A.; Morrey, Luke B.; Riester, Scott M.; Trousdale, William H.; Salib, Christopher G.; Reina, Nicolas; Yaszemski, Michael J.; Sánchez-Sotelo, Joaquín; Morrey, Mark E.; Wijnen, André J. van; Abdel, Matthew P.

doi:10.1016/j.genrep.2017.05.006

Cited by 1 publication

(1 citation statement)

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“…Finally, a 1.6‐mm K‐wire was placed through the tibia and hooked around the femur to immobilize the knee in 160° of flexion. We have previously published the ability of this technique to induce a severe joint contracture for 24 weeks …”

Section: Methodsmentioning

confidence: 99%

Validation of a dynamic joint contracture measuring device in a live rabbit model of arthrofibrosis

Reina

Trousdale

Salib

et al. 2018

Journal Orthopaedic Research

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The current method of measuring arthrofibrosis in live rabbits is critically limited. Specifically, this method involves radioactive fluoroscopy, error-prone goniometric measurements, and static joint angle outcomes that fail to approximate the compliance of tissues surrounding the joint. This study aims to validate a novel method of capturing the compliance of contracted tissues surrounding the joint without the use of fluoroscopy or animal sacrifice. Surgically induced contractures of one-hundred and eight rabbits were measured using the current standard of contracture measurement (a pulley system) as well as a newly designed dynamic load cell (DLC) device. The DLC device was highly reliable when compared to the pulley system (r = 0.907, p < 0.001). Finally, the DLC device produced joint stiffness hysteresis curves capable of approximating the compliance of stiff joint tissues, ultimately calculating a mean joint stiffness of 1.57 ± 1.31 N · m · rad (range, 0.33-6.37 N · m · rad ). In conclusion, the DLC device represents a valid method for measuring joint contractures. Further, the DLC device notably improves current techniques by introducing the capacity to approximate the compliance of contracted tissues in living rabbits. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.

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Section: Methodsmentioning

confidence: 99%