Biopolymer deposition for freeform fabrication of hydrogel tissue constructs

Khalil, Saif; Sun, Wei

doi:10.1016/j.msec.2006.05.023

Cited by 217 publications

(133 citation statements)

References 25 publications

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“…in which various stimuli are used to induce rapid solidification of deposited build materials in situ, such as temperature change [14,15], ultraviolet (UV) radiation [16,17], and ionic cross-linking [10,18]. However, this fabrication methodology follows the traditional "solidification-while-printing" procedure and has some constraints including the requirement of rapid solidification of liquid build materials in order to retain the printed shape and nozzle clogging.…”

mentioning

confidence: 99%

“…As the internal scaffold material during extrusion, Laponite XLG is added to prepare the NIPAAm-Laponite nanocomposite hydrogel precursor to investigate the improvement of the bioink extrudability. For comparison, high-concentration NaAlg, a natural polysaccharide widely used for biorelated applications [6,7,9,10,18], is selected as a benchmark bioink material herein. In summary, four biocompatible materials (NIPAAm, Laponite, NIPAAm-Laponite, and NaAlg) are extruded through a transparent glass nozzle to study their extrudability, and the velocity field distribution is measured to evaluate the effects of nanoclay on the extrudability.…”

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confidence: 99%

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Study of extrudability and standoff distance effect during nanoclay-enabled direct printing

2018

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Nanoclay-enabled self-supporting printing has been emerging as a promising filament-based extrusion fabrication approach for different biomedical and engineering applications including tissue engineering. With the addition of nanoclay powders, liquid build materials may exhibit solid-like behavior upon extrusion and can be directly printed in air into complex three-dimensional structures. The objective of this study is to investigate the effect of nanoclay on the extrudability of N -isopropylacrylamide (NIPAAm) and the effect of standoff distance on the print quality during nanoclay-enabled direct printing. It is found that the addition of nanoclay can significantly improve the NIPAAm extrudability and effectively eliminate die swelling in material extrusion. In addition, with the increase of standoff distance, deposited filaments change from over-deposited to well-defined to stretched to broken, the filament width decreases, and the print fidelity deteriorates. A mathematical model is further proposed to determine the optimal standoff distance to achieve better print fidelity during nanoclay-enabled direct printing. Based on the extrudability and standoff distance knowledge from this study, NIPAAm-Laponite nanoclay and NIPAAm-Laponite nanoclay-graphene oxide nanocomposite hydrogel precursors are successfully printed into a three-layered one-dimensional responsive pattern, demonstrating the good extrudability and print quality during nanoclay-enabled printing under optimal printing conditions.

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confidence: 99%

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confidence: 99%

Study of extrudability and standoff distance effect during nanoclay-enabled direct printing

2018

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“…These bioprinted structures retained structural integrity for over 2 months in culture, with a cell survival rate of over 95%. Sun and colleagues printed rat heart endothelial cells in an alginate hydrogel that were able to proliferate sixfold to infill the hydrogel scaffold [45]. Perhaps the most exciting application of extrusion bioprinting is the fabrication of branching vascular networks.…”

Section: Cd31 Pkh26 40xmentioning

confidence: 99%

Bioprinting: Uncovering the Utility Layer-By-Layer

Carter¹,

Burke²,

Perriman³

2017

J. 3D Print. Med.

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ReviewBioprinting is becoming a must have capability in tissue engineering research. Key to the growth of the field is the inherent flexibility, which can be used to answer basic scientific questions that can only be addressed under 3D culture conditions, or organon-chip systems that could quickly replace underperforming animal models. Almost certainly the most challenging application of bioprinting will be for bottom-up tissue construction, which faces many of the same challenges as scaffold-based tissue engineering. In this review, the current state-of-the-art approaches to 3D bioprinting are discussed in terms of performance and suitability. This is complemented by an overview of hydrogel-based bioinks, with a special emphasis on composite biomaterial systems.

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“…Two main dispensing systems that are used to extrude biomaterials are mechanical and pneumatic [35] (Figure 3). The bioink flow is better managed in mechanical dispensing rather than pneumatic dispensing method [36,37] . The compressed gas volume in the pneumatic system can delay the ink flow.…”

Section: Microextrusionmentioning

confidence: 99%

3D bioprinting technology for regenerative medicine applications

Sundaramurthi¹,

Rauf²,

Hauser³

2016

IJB

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Alternative strategies that overcome existing organ transplantation methods are of increasing importance because of ongoing demands and lack of adequate organ donors. Recent improvements in tissue engineering techniques offer improved solutions to this problem and will influence engineering and medicinal applications. Tissue engineering employs the synergy of cells, growth factors and scaffolds besides others with the aim to mimic the native extracellular matrix for tissue regeneration. Three-dimensional (3D) bioprinting has been explored to create organs for transplantation, medical implants, prosthetics, in vitro models and 3D tissue models for drug testing. In addition, it is emerging as a powerful technology to provide patients with severe disease conditions with personalized treatments. Challenges in tissue engineering include the development of 3D scaffolds that closely resemble native tissues. In this review, existing printing methods such as extrusion-based, robotic dispensing, cellular inkjet, laser-assisted printing and integrated tissue organ printing (ITOP) are examined. Also, natural and synthetic polymers and their blends as well as peptides that are exploited as bioinks are discussed with emphasis on regenerative medicine applications. Furthermore, applications of 3D bioprinting in regenerative medicine, evolving strategies and future perspectives are summarized.

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Biopolymer deposition for freeform fabrication of hydrogel tissue constructs

Cited by 217 publications

References 25 publications

Study of extrudability and standoff distance effect during nanoclay-enabled direct printing

Study of extrudability and standoff distance effect during nanoclay-enabled direct printing

Bioprinting: Uncovering the Utility Layer-By-Layer

3D bioprinting technology for regenerative medicine applications

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