Biopolymer networks packed with microgels combine strain stiffening and shape programmability

Subramaniam, Vignesh; Shetty, Abhishek M.; Chisolm, Steven J.; Lansberry, Taylor R.; Balachandar, Anjana; Morley, Cameron D.; Angelini, Thomas E.

doi:10.1016/j.giant.2024.100297

Giant

2024

DOI: 10.1016/j.giant.2024.100297

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Biopolymer networks packed with microgels combine strain stiffening and shape programmability

Vignesh Subramaniam,

Abhishek M. Shetty,

Steven J. Chisolm

et al.

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Determining Rates of Molecular Secretion from Supernatant Concentration Measurements in a 3D-Bioprinted Human Liver Tissue Model

Anjum,

Subramaniam,

Higgins

et al. 2024

ACS Biomater. Sci. Eng.

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The secretion rate of albumin is a key indicator of function in liver tissue models used for hepatotoxicity and pharmacokinetic testing. However, it is not generally clear how to determine molecular secretion rates from measurements of the molecular concentration in supernatant media. Here, we develop computational and analytical models of molecular transport in an experimental system that enable determination of albumin secretion rates based on measurements of albumin concentration in supernatant media. The experimental system is a 3D-bioprinted human liver tissue construct embedded in a 3D culture environment made from packed microgel particles swollen in liquid growth media. The mathematical models reveal that the range of albumin synthesis rates necessary to match experimentally measured albumin concentrations corresponds to reaction-limited conditions, where a steady state of albumin spatial distribution is rapidly reached between media exchanges. Our results show that temporally resolved synthesis rates can be inferred from serial concentration measurements of collected supernatant media. This link is critical to confidently assessing in vitro tissue performance in applications where critical quality attributes must be quantified, like in drug development and screening.

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Determining Rates of Molecular Secretion from Supernatant Concentration Measurements in a 3D-Bioprinted Human Liver Tissue Model

Anjum,

Subramaniam,

Higgins

et al. 2024

ACS Biomater. Sci. Eng.

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show abstract

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Biopolymer networks packed with microgels combine strain stiffening and shape programmability

Cited by 1 publication

References 50 publications

Determining Rates of Molecular Secretion from Supernatant Concentration Measurements in a 3D-Bioprinted Human Liver Tissue Model

Determining Rates of Molecular Secretion from Supernatant Concentration Measurements in a 3D-Bioprinted Human Liver Tissue Model

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