Biopolymer Synthesis on Polypropylene Supports

Matson, Robert S.; Rampal, Jang B.; Coassin, Peter J.

doi:10.1006/abio.1994.1123

Cited by 53 publications

(31 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oligonucleotide array (complement of 111 nt including positions 114-224 in the Cyclin B5 mRNA) was prepared directly onto the surface of aminated polypropylene (Matson et al+, 1994) on an adapted Applied Biosystem's DNA synthesizer (ABI 381 A) using a 42-mm diamond-shaped mask as described previously (Southern et al+, 1994)+ Standard phosphoramidites were used in the synthesis+ The offset between each coupling step was 2 mm and thus the longest oligonucleotides produced were 21-mers+ The arrays were deprotected in 30% ammonia solution at 55 8C for 16 h in a closed chamber (Southern et al+, 1994)+…”

Section: Fabrication Of Arraysmentioning

confidence: 99%

The folding of large RNAs studied by hybridization to arrays of complementary oligonucleotides

Sohail¹,

Akhtar²,

Southern³

1999

RNA

View full text Add to dashboard Cite

Folding pathways of large RNAs are poorly understood. We have addressed this question by hybridizing in vitro transcripts, which varied in size, to an array of antisense oligonucleotides. All transcripts included a common sequence and all but one shared the same start-point; the other had a small deletion of the 59 end. Minimal free energy calculations predicted quite different folds for these transcripts. However, hybridization to the array showed predominant features that were shared by transcripts of all lengths, though some oligonucleotides that hybridized strongly to the short transcripts gave weak interaction with longer transcripts. A full-length RNA fragment that had been denatured by heating and allowed to cool slowly gave the same hybridization result as a shorter transcript. Taken together, these results support theories that RNA folding creates local stable states that are trapped early in the transcription or folding process. As the transcript elongates, interactions are added between regions that are transcribed early and those transcribed late. The method here described helps in identifying regions in the transcripts that take part in long-range interactions.

show abstract

Section: Fabrication Of Arraysmentioning

confidence: 99%

The folding of large RNAs studied by hybridization to arrays of complementary oligonucleotides

Sohail¹,

Akhtar²,

Southern³

1999

RNA

View full text Add to dashboard Cite

show abstract

“…This surface density gives an average distance of ~18 Å between adjacent thiocyanate groups, which is nearly same as the diameter of the DNA helix. 15,[26][27][28] On the other hand, two-dimensional solid supports have the surface density of terminal functional groups of ~0.3 nmol/cm 2 with the distance of ~10 Å between adjacent functional groups.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Oligonucleotide Arrays with High-Density DNA Deposition and High Hybridization Efficiency

2004

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

In DNA microarray produced by DNA-deposition technology, DNA-immobilization and -hybridization yields on a solid support are most important factors for its accuracy and sensitivity. We have developed a dendrimeric support using silylated aldehyde slides and polyamidoamine (PAMAM) dendrimers. An oligonucleotide array was prepared through a crosslinking between the dendrimeric support and an oligonucleotide. Both DNAimmobilization and -hybridization yields on the solid support increased by the modification with the dendrimers. The increase of the immobilization and hybridization efficiency seems to result from a threedimensional arrangement of the attached oligonucleotide. Therefore, our dendrimeric support may provide a simple and efficient solution to the preparation of DNA microarrays with high-density DNA-deposition and high hybridization efficiency.

show abstract

“…[8] However polymeric materials are promising alternatives because of their low density, high elasticity and toughness, easy processability and low cost. Several plastics have been investigated for covalent or non-covalent immobilization of oligonucleotide probes, such as for example polypropylene, [9] polymethylmethacrylate [10,11] and polycarbonate. [12] Poly- Model high density DNA arrays have been realized by direct deposition with Dip-Pen Nanolithography of acrylamido-functionalized oligonucleotides (23-mer) on spin-coated, flat polystyrene surfaces.…”

Section: Introductionmentioning

confidence: 99%

Towards Nanowriting on Plastics: Dip‐Pen Nanolithography of Acrylamido‐Functionalized Oligonucleotides on Polystyrene

Turri

Torlaj

Levi

2010

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Model high density DNA arrays have been realized by direct deposition with Dip-Pen Nanolithography of acrylamido-functionalized oligonucleotides (23-mer) on spin-coated, flat polystyrene surfaces. A highly specific interaction between the acrylamide end functionality and polystyrene was found. The surface morphology of the model array was studied by atomic force microscopy (AFM). Spots are clearly seen both in topography and demodulation modes. The array withstands the hybridization process with label free, complementary oligonucleotides and the following cleaning procedures. The final AFM characterization showed significant changes especially in demodulation images which may be an indication that molecular recognition between complementary oligos has occurred.

show abstract

Biopolymer Synthesis on Polypropylene Supports

Cited by 53 publications

References 0 publications

The folding of large RNAs studied by hybridization to arrays of complementary oligonucleotides

The folding of large RNAs studied by hybridization to arrays of complementary oligonucleotides

Preparation of Oligonucleotide Arrays with High-Density DNA Deposition and High Hybridization Efficiency

Towards Nanowriting on Plastics: Dip‐Pen Nanolithography of Acrylamido‐Functionalized Oligonucleotides on Polystyrene

Contact Info

Product

Resources

About