Biopolymers recovery: dynamics and characterization of alginate-like exopolymers in an aerobic granular sludge system treating municipal wastewater without sludge inoculum

Schambeck, Cássio Moraes; Magnus, Bruna Scandolara; Souza, Liliete Canes; Leite, Wanderli Rogério Moreira; Derlon, Nicolas; Guimarães, Lorena; Costa, Rejane Helena Ribeiro da

doi:10.1016/j.jenvman.2020.110394

Cited by 66 publications

(13 citation statements)

References 57 publications

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“…The fraction of industrial wastewater in the influent (here given as the COD fraction) (Figure 2D) was also shown as an important and statistically significant factor influencing the abundance of all the species, based on Kruskal-Wallis statistical analysis. All species were found mainly in municipal plants with low or no influx of industrial wastewater (<30%) (Figure 2D) in agreement with other studies (Yang et al, 2017;Chi et al, 2018;Luo et al, 2020;Schambeck et al, 2020).…”

Section: Abundance and Distribution Of Saprospiraceae In Wwtps Worldwidesupporting

confidence: 92%

Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

et al. 2022

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The Saprospiraceae family within the phylum Bacteroidota is commonly present and highly abundant in wastewater treatment plants (WWTPs) worldwide, but little is known about its role. In this study, we used MiDAS 4 global survey with samples from 30 countries to analyze the abundance and distribution of members of Saprospiraceae. Phylogenomics were used to delineate five new genera from a set of 31 high-quality metagenome-assembled genomes from Danish WWTPs. Newly designed probes for fluorescence in situ hybridization (FISH) revealed rod-shaped morphologies for all genera analyzed, including OLB8, present mostly inside the activated sludge flocs. The genomes revealed potential metabolic capabilities for the degradation of polysaccharides, proteins, and other complex molecules; partial denitrification; and storage of intracellular polymers (glycogen, polyphosphate, and polyhydroxyalkanoates). FISH in combination with Raman microspectroscopy confirmed the presence of intracellular glycogen in Candidatus Brachybacter, Candidatus Parvibacillus calidus (both from the former genus OLB8), and Candidatus Opimibacter, and the presence of polyhydroxyalkanoates in Candidatus Defluviibacterium haderslevense and Candidatus Vicinibacter. These results provide the first overview of the most abundant novel Saprospiraceae genera present in WWTPs across the world and their potential involvement in nutrient removal and the degradation of macromolecules.

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Section: Abundance and Distribution Of Saprospiraceae In Wwtps Worldwidesupporting

confidence: 92%

Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

et al. 2022

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“…As to polysaccharides, two crucial constituents i.e., alginate-like exopolysaccharide (ALE) (Lin et al, 2010;Schambeck et al, 2020) and granulan (Seviour et al, 2010) have been identified as the functional gel-forming constituents in AGS. Despite granulan and ALE display structural hydrogels properties, they are completely distinct.…”

Section: High Diversity Of Gs-derived Eps Contents: Emerging Classifi...mentioning

confidence: 99%

Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge

Lotti

Carretti

Berti

et al. 2019

Journal of Environmental Management

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“…The coexistence of multiple granule morphologies is common in granular sludge bioreactors. This was reported for phototrophic algal–bacterial biomass under batch or continuous cultivation with synthetic medium (Brehm et al, 2003) or real wastewater (Arcila & Buitrón, 2017) for anammox sequencing batch reactors treating municipal wastewater (Pijuan et al, 2020) or sequencing batch reactors with aerobic granular sludge treating synthetic or real wastewater (Layer et al, 2019; Pronk et al, 2015; Schambeck et al, 2020). In our photobioreactor, we observed the coexistence of a range of morphologies, from filament‐covered photogranules to nearly spherical photogranules (Figure 1).…”

Section: Discussionmentioning

confidence: 84%

Mapping the biological activities of filamentous oxygenic photogranules

Ouazaite

Milferstedt

Hamelin

et al. 2020

Biotech & Bioengineering

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Oxygenic photogranules have been suggested as alternatives to activated sludge in wastewater treatment. Challenging for modeling photogranule‐based processes is the heterogeneity of photogranule morphologies, resulting in different activities by photogranule type. The measurement of microscale‐activities of filamentous photogranules is particularly difficult because of their labile interfaces. We present here an experimental and modeling approach to quantify phototrophic O2 production, heterotrophic O2 consumption, and O2 diffusion in filamentous photogranules. We used planar optodes for the acquisition of spatio‐temporal oxygen distributions combined with two‐dimensional mathematical modeling. Light penetration into the photogranule was the factor controlling photogranule activities. The spatial distribution of heterotrophs and phototrophs had less impact. The photosynthetic response of filaments to light was detectable within seconds, emphasizing the need to analyze dynamics of light exposure of individual photogranules in photobioreactors. Studying other recurring photogranule morphologies will eventually enable the description of photogranule‐based processes as the interplay of interacting photogranule populations.

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Biopolymers recovery: dynamics and characterization of alginate-like exopolymers in an aerobic granular sludge system treating municipal wastewater without sludge inoculum

Cited by 66 publications

References 57 publications

Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge

Mapping the biological activities of filamentous oxygenic photogranules

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