Bioprinting of Cartilage with Bioink Based on High-Concentration Collagen and Chondrocytes

Бекетов, Е. Е.; Isaeva, E. V.; Yakovleva, N. D.; Demyashkin, Grigory; Arguchinskaya, Nadezhda V.; Kisel, Anastas A.; Lagoda, Tatiana S.; Malakhov, Egor P.; Kharlov, Valentin I.; Osidak, E. O.; Domogatsky, S.P.; Иванов, С. А.; Shegay, Peter; Каприн, А. Д.

doi:10.3390/ijms222111351

Cited by 27 publications

(18 citation statements)

References 43 publications

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“…In all groups, in one of the two animals euthanized after one week, we observed osteogenesis with the formation of coarse fibrous bone tissue, which occurs mainly in the embryos. We also observed bone formation, in addition to cartilaginous tissue, in our previous study, after subcutaneous implantation of scaffolds containing 4% type I collagen and rat chondrocytes in rats [ 60 ]. This is probably due to the peculiarities of the molecular mechanisms of regulation of osteo- and chondrogenesis in specific animals, since MSC derived from adipose tissue are equally capable of differentiating into cells such as osteoblasts, chondroblasts, and adipocytes [ 61 ].…”

Section: Discussionmentioning

confidence: 67%

Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules

Isaeva

Бекетов

Demyashkin

et al. 2022

IJMS

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The aim of this study was to verify the applicability of high-concentration collagen-based bioink with MSC (ADSC) and decellularized ECM granules for the formation of cartilage tissue de novo after subcutaneous implantation of the scaffolds in rats. The printability of the bioink (4% collagen, 2.5% decellularized ECM granules, derived via 280 μm sieve) was shown. Three collagen-based compositions were studied: (1) with ECM; (2) with MSC; (3) with ECM and MSC. It has been established that decellularized ECM granules are able to stimulate chondrogenesis both in cell-free and MSC-laden scaffolds. Undesirable effects have been identified: bone formation as well as cartilage formation outside of the scaffold area. The key perspectives and limitations of ECM granules (powder) application have been discussed.

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Section: Discussionmentioning

confidence: 67%

Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules

Isaeva

Бекетов

Demyashkin

et al. 2022

IJMS

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“…There are several previous studies using 3D printers. Beketov et al applied bioprinting on de novo cartilage formation [ 21 ]. They printed a bioink mixed with chondrocytes and 4% collagen and conducted a small animal experiment.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Three-Dimensional Bioprinted Human Cartilage Powder Combined with Micronized Subcutaneous Adipose Tissues for the Repair of Osteochondral Defects in Beagle Dogs

Ryu¹,

Brittberg

Nam³

et al. 2022

IJMS

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Cartilage lesions are difficult to repair due to low vascular distribution and may progress into osteoarthritis. Despite numerous attempts in the past, there is no proven method to regenerate hyaline cartilage. The purpose of this study was to investigate the ability to use a 3D printed biomatrix to repair a critical size femoral chondral defect using a canine weight-bearing model. The biomatrix was comprised of human costal-derived cartilage powder, micronized adipose tissue, and fibrin glue. Bilateral femoral condyle defects were treated on 12 mature beagles staged 12 weeks apart. Four groups, one control and three experimental, were used. Animals were euthanized at 32 weeks to collect samples. Significant differences between control and experimental groups were found in both regeneration pattern and tissue composition. In results, we observed that the experimental group with the treatment with cartilage powder and adipose tissue alleviated the inflammatory response. Moreover, it was found that the MOCART score was higher, and cartilage repair was more organized than in the other groups, suggesting that a combination of cartilage powder and adipose tissue has the potential to repair cartilage with a similarity to normal cartilage. Microscopically, there was a well-defined cartilage-like structure in which the mid junction below the surface layer was surrounded by a matrix composed of collagen type I, II, and proteoglycans. MRI examination revealed significant reduction of the inflammation level and progression of a cartilage-like growth in the experimental group. This canine study suggests a promising new surgical treatment for cartilage lesions.

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“…3D printing is a promising technique for the formation of personalized grafts for cartilage and bone regeneration using hydrogels, bone chips, laser-sintered titanium [ 93 , 94 , 95 , 96 ]. Traditionally, 3D bioprinting involves cells cultured in vitro.…”

Section: Methods Of Intraoperative Cell Seeding On Scaffoldsmentioning

confidence: 99%

Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ

et al. 2022

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Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for the translation of biomedical technologies into clinical practice. These intraoperative approaches could be generally characterized as a joint concept of tissue engineering in situ. Our review covers techniques of intraoperative cell isolation and seeding for the creation of tissue-engineered grafts in situ, that is, directly in the Operating Room. Up-to-date, the clinical use of tissue-engineered grafts created in vitro remains a highly inaccessible option. Fortunately, intraoperative tissue engineering in situ is already available for patients who need advanced treatment modalities.

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Bioprinting of Cartilage with Bioink Based on High-Concentration Collagen and Chondrocytes

Cited by 27 publications

References 43 publications

Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules

Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules

Evaluation of Three-Dimensional Bioprinted Human Cartilage Powder Combined with Micronized Subcutaneous Adipose Tissues for the Repair of Osteochondral Defects in Beagle Dogs

Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ

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