Bioprinting with bioactive alginate dialdehyde-gelatin (ADA-GEL) composite bioinks: Time-dependent in-situ crosslinking via addition of calcium-silicate particles tunes in vitro stability of 3D bioprinted constructs

Heid, Susanne; Becker, Kevin G.; Byun, Jun Soo; Biermann, Isabell; Neščaková, Zuzana; Zhu, Hongqin; Groll, Jürgen; Boccaccını, Aldo R.

doi:10.1016/j.bprint.2022.e00200

Cited by 28 publications

(13 citation statements)

References 91 publications

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“…Besides, significant differences were found between day one and day 28 in all scaffold groups. The biocompatible polymer combination of ADA-GEL is capable of inducing cell proliferation as proven previously [60]. Additionally, the swelling capacity of hydrogels demonstrated in figure 5(B) could also support cell proliferation.…”

Section: Ada-gel/fs Hydrogels Allow Cell Proliferation With High Cell...supporting

confidence: 69%

Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering

et al. 2023

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The development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for bioprinting technology in tissue engineering applications. The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels. However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications. To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications. We fabricate 3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells. We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels. Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increases with increasing FS particle content. Addition of FS also provides a tool to tune hydrogel stiffness. FS particles were homogeneously incorporated into the hydrogels. Particle-matrix integration was confirmed via scanning electron microscopy. FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells. Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications. Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.

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Section: Ada-gel/fs Hydrogels Allow Cell Proliferation With High Cell...supporting

confidence: 69%

Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering

et al. 2023

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“…Alginate dialdehyde (ADA) was also synthesized following Heid et al [ 44 ]. Alginate was dissolved in ethanol followed by the addition of dropwise sodium metaperiodate which was previously dissolved by adding 1.337 g in 50 mL MilliQ ® water.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of Hydrogel-Based Composite Fibers and Computer Simulation of the Filler Dynamics in the Composite Flow

et al. 2023

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Fibrous structures with anisotropic fillers as composites have found increasing interest in the field of biofabrication since they can mimic the extracellular matrix of anisotropic tissues such as skeletal muscle or nerve tissue. In the present work, the inclusion of anisotropic fillers in hydrogel-based filaments with an interpenetrating polymeric network (IPN) was evaluated and the dynamics of such fillers in the composite flow were analyzed using computational simulations. In the experimental part, microfabricated rods (200 and 400 μm length, 50 μm width) were used as anisotropic fillers in extrusion of composite filaments using two techniques of wet spinning and 3D printing. Hydrogels such as oxidized alginate (ADA) and methacrylated gelatin (GelMA) were used as matrices. In the computational simulation, a combination of computational fluid dynamics and coarse-grained molecular dynamics was used to study the dynamics of rod-like fillers in the flow field of a syringe. It showed that, during the extrusion process, microrods are far from being well aligned. Instead, many of them tumble on their way through the needle leading to a random orientation in the fiber which was confirmed experimentally.

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“…This causes increased ECM formation, allowing regenerated tissue to replace the decomposed scaffold [ 172 ]. In this context, structures can be obtained by several techniques, including the freeze-drying method [ 173 ], electrospinning [ 174 ], foaming [ 175 ], sol-gel [ 176 ], 3D printing [ 177 ], or by the use of pyrogenic agents such as PMMA [ 178 ]. More recently, 3D/4D printed scaffolds have become more widespread in the scientific environment due to the greater precision provided by the technique for controlling material properties, such as porosity and pore size, degradation profile, drug release and others, thus being able to create structures similar to living tissue [ 165 , 179 , 180 , 181 ].…”

Section: Composites Of Gelatin/bg For Tissue Engineeringmentioning

confidence: 99%

Gelatin and Bioactive Glass Composites for Tissue Engineering: A Review

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et al. 2022

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Nano-/micron-sized bioactive glass (BG) particles are attractive candidates for both soft and hard tissue engineering. They can chemically bond to the host tissues, enhance new tissue formation, activate cell proliferation, stimulate the genetic expression of proteins, and trigger unique anti-bacterial, anti-inflammatory, and anti-cancer functionalities. Recently, composites based on biopolymers and BG particles have been developed with various state-of-the-art techniques for tissue engineering. Gelatin, a semi-synthetic biopolymer, has attracted the attention of researchers because it is derived from the most abundant protein in the body, viz., collagen. It is a polymer that can be dissolved in water and processed to acquire different configurations, such as hydrogels, fibers, films, and scaffolds. Searching “bioactive glass gelatin” in the tile on Scopus renders 80 highly relevant articles published in the last ~10 years, which signifies the importance of such composites. First, this review addresses the basic concepts of soft and hard tissue engineering, including the healing mechanisms and limitations ahead. Then, current knowledge on gelatin/BG composites including composition, processing and properties is summarized and discussed both for soft and hard tissue applications. This review explores physical, chemical and mechanical features and ion-release effects of such composites concerning osteogenic and angiogenic responses in vivo and in vitro. Additionally, recent developments of BG/gelatin composites using 3D/4D printing for tissue engineering are presented. Finally, the perspectives and current challenges in developing desirable composites for the regeneration of different tissues are outlined.

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Bioprinting with bioactive alginate dialdehyde-gelatin (ADA-GEL) composite bioinks: Time-dependent in-situ crosslinking via addition of calcium-silicate particles tunes in vitro stability of 3D bioprinted constructs

Cited by 28 publications

References 91 publications

Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering

Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering

Fabrication of Hydrogel-Based Composite Fibers and Computer Simulation of the Filler Dynamics in the Composite Flow

Gelatin and Bioactive Glass Composites for Tissue Engineering: A Review

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