Bioprocess development for scalable production of cultivated meat

Hanga, Mariana P.; Ali, J.; Moutsatsou, Panagiota; Raga, Fritz Anthony de la; Hewitt, Christopher J.; Nienow, Alvin W.; Wall, Ivan

doi:10.1002/bit.27469

Cited by 53 publications

(38 citation statements)

References 44 publications

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“…Other cells that have been grown successfully on microcarriers using the same growth and detachment protocols [8,54] are bovine adiposederived stem cells (bASCs) [58] in spinner flasks. This process is the first step in the production of cultivated meat, which mimics animal meat, while being produced in a bioreactor under controlled conditions, rather than through slaughtering animals.…”

Section: Animal Cells On Microcarriers: Stem Cellsmentioning

confidence: 99%

The Impact of Fluid Dynamic Stress in Stirred Bioreactors – The Scale of the Biological Entity: A Personal View

Nienow

2020

Chemie Ingenieur Technik

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From the start of industrial biotechnology, there has been a perception that biological entities are damaged by stirring, so‐called ‘shear damage'. Often, it was the soft option to explain a loss of performance when it was due to other factors, such as bubble ingestion with proteins or on scale‐up, where tip speed increased when it was due to decreased homogeneity, especially in pH. For many years, poor control and the range of analytical tools available made a more in‐depth explanation difficult; and the concepts of ‘high' and ‘low shear' impellers, now largely disproven, increased it. Here, the size of the biological entity is compared to the Kolmogorov microscale of turbulence leading to a reasonably clear picture emerging. The article starts with the author's introduction to the issue approximately 42 year ago with enzymes, conveniently also the smallest entity; and finishes with the largest, filamentous fungi.

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Section: Animal Cells On Microcarriers: Stem Cellsmentioning

confidence: 99%

The Impact of Fluid Dynamic Stress in Stirred Bioreactors – The Scale of the Biological Entity: A Personal View

Nienow

2020

Chemie Ingenieur Technik

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“…Perfusion bioreactors function by providing upstream medium flow that retains the cells inside the bioreactor vessel while simultaneously removing cell waste products and conditioned medium, which aids in maximizing vessel volume and medium recycling. This type of bioreactor may also be mimicked by regularly replacing culture medium in the bioreactor at discrete time points without harvesting cells, which has been shown to be a promising method for cultivated meat production [ 23 ]. For large-scale applications, such as cultivated meat production, fed-batch or continuous medium introduction are generally favored as they better support large volumes, can be more easily automated, and allow for recycling of conditioned medium [ 100 ].…”

Section: Cell Sources For Cultivated Meat Productionmentioning

confidence: 99%

Cell Sources for Cultivated Meat: Applications and Considerations throughout the Production Workflow

Reiss

Robertson

Suzuki

2021

IJMS

113

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Cellular agriculture is an emerging scientific discipline that leverages the existing principles behind stem cell biology, tissue engineering, and animal sciences to create agricultural products from cells in vitro. Cultivated meat, also known as clean meat or cultured meat, is a prominent subfield of cellular agriculture that possesses promising potential to alleviate the negative externalities associated with conventional meat production by producing meat in vitro instead of from slaughter. A core consideration when producing cultivated meat is cell sourcing. Specifically, developing livestock cell sources that possess the necessary proliferative capacity and differentiation potential for cultivated meat production is a key technical component that must be optimized to enable scale-up for commercial production of cultivated meat. There are several possible approaches to develop cell sources for cultivated meat production, each possessing certain advantages and disadvantages. This review will discuss the current cell sources used for cultivated meat production and remaining challenges that need to be overcome to achieve scale-up of cultivated meat for commercial production. We will also discuss cell-focused considerations in other components of the cultivated meat production workflow, namely, culture medium composition, bioreactor expansion, and biomaterial tissue scaffolding.

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“…It is worth mentioning that prior to the use of expensive liter scale bioreactors, the bioprocess development is usually performed in spinner flasks that use impeller-driven agitation and are of considerably smaller volumes (up to 500 mL). Hanga et al used spinner flasks for the cultivation of bovine adipose-derived stem cells (bASCs) on microcarriers [72].…”

Section: Types Of Bioreactorsmentioning

confidence: 99%

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Djisalov

Knežić

Podunavac

et al. 2021

Biology

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Meat cultivation via cellular agriculture holds great promise as a method for future food production. In theory, it is an ideal way of meat production, humane to the animals and sustainable for the environment, while keeping the same taste and nutritional values as traditional meat and having additional benefits such as controlled fat content and absence of antibiotics and hormones used in the traditional meat industry. However, in practice, there is still a number of challenges, such as those associated with the upscale of cultured meat (CM). CM food safety monitoring is a necessary factor when envisioning both the regulatory compliance and consumer acceptance. To achieve this, a multidisciplinary approach is necessary. This includes extensive development of the sensitive and specific analytical devices i.e., sensors to enable reliable food safety monitoring throughout the whole future food supply chain. In addition, advanced monitoring options can help in the further optimization of the meat cultivation which may reduce the currently still high costs of production. This review presents an overview of the sensor monitoring options for the most relevant parameters of importance for meat cultivation. Examples of the various types of sensors that can potentially be used in CM production are provided and the options for their integration into bioreactors, as well as suggestions on further improvements and more advanced integration approaches. In favor of the multidisciplinary approach, we also include an overview of the bioreactor types, scaffolding options as well as imaging techniques relevant for CM research. Furthermore, we briefly present the current status of the CM research and related regulation, societal aspects and challenges to its upscaling and commercialization.

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Bioprocess development for scalable production of cultivated meat

Cited by 53 publications

References 44 publications

The Impact of Fluid Dynamic Stress in Stirred Bioreactors – The Scale of the Biological Entity: A Personal View

The Impact of Fluid Dynamic Stress in Stirred Bioreactors – The Scale of the Biological Entity: A Personal View

Cell Sources for Cultivated Meat: Applications and Considerations throughout the Production Workflow

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

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