Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

Boodhoo, Kamelia; Flickinger, Michael C.; Woodley, John M.; Emanuelsson, Emma Anna Carolina

doi:10.1016/j.cep.2022.108793

Cited by 62 publications

(25 citation statements)

References 250 publications

(338 reference statements)

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“…The increase of the overall productivity of the prototype of the integrated biocatalytic procedure is essential for reaching a viable manufacturing process. The intensification of biocatalytic processes needs to take into account upstream, biocatalytic reaction and downstream phases (Boodhoo et al, 2022). The use of simple process metrics such as reaction yield, space-time yield, product concentration (Figure 6A) is thereby not only valuable for characterizing and comparing process performance in the course of intensifying, scaling and implementing biocatalytic processes, but also for setting targets for protein engineering and process engineering (Woodley 2017).…”

Section: Intensification Of Biocatalytic Processesmentioning

confidence: 99%

Complexity reduction and opportunities in the design, integration and intensification of biocatalytic processes for metabolite synthesis

Wohlgemuth

Littlechild²

2022

Front. Bioeng. Biotechnol.

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The biosynthesis of metabolites from available starting materials is becoming an ever important area due to the increasing demands within the life science research area. Access to metabolites is making essential contributions to analytical, diagnostic, therapeutic and different industrial applications. These molecules can be synthesized by the enzymes of biological systems under sustainable process conditions. The facile synthetic access to the metabolite and metabolite-like molecular space is of fundamental importance. The increasing knowledge within molecular biology, enzyme discovery and production together with their biochemical and structural properties offers excellent opportunities for using modular cell-free biocatalytic systems. This reduces the complexity of synthesizing metabolites using biological whole-cell approaches or by classical chemical synthesis. A systems biocatalysis approach can provide a wealth of optimized enzymes for the biosynthesis of already identified and new metabolite molecules.

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Section: Intensification Of Biocatalytic Processesmentioning

confidence: 99%

Complexity reduction and opportunities in the design, integration and intensification of biocatalytic processes for metabolite synthesis

Wohlgemuth

Littlechild²

2022

Front. Bioeng. Biotechnol.

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“…Current protein yields using plant or microbial fermentation synthesis are still insufficient to produce a fully viable synthetic protein source for poultry. However, the efficiency of these processes is rapidly improving, and new developments in bioprocesses are emerging [ 62 ], as are innovative procedures, such as cell-free protein synthesis [ 63 ]. The use of these novel protein synthesis methods could contribute to making this procedure a reality in the near future.…”

Section: Case Study 2: De Novo Protein Design An E...mentioning

confidence: 99%

Biomarkers and De Novo Protein Design Can Improve Precise Amino Acid Nutrition in Broilers

Cambra‐López

Marín-García

Lledó

et al. 2022

Animals

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Precision nutrition in broilers requires tools capable of identifying amino acid imbalances individually or in groups, as well as knowledge on how more digestible proteins can be designed for innovative feeding programs adjusted to animals’ dynamic requirements. This work proposes two potential tools, combining traditional nutrition with biotechnological, metabolomic, computational and protein engineering knowledge, which can contribute to improving the precise amino acid nutrition of broilers in the future: (i) the use of serum uric nitrogen content as a rapid biomarker of amino acid imbalances, and (ii) the design and modeling of de novo proteins that are fully digestible and fit exactly to the animal’s requirements. Each application is illustrated with a case study. Case study 1 demonstrates that serum uric nitrogen can be a useful rapid indicator of individual or group amino acid deficiencies or imbalances when reducing dietary protein and adjusting the valine and arginine to lysine ratios in broilers. Case study 2 describes a stepwise approach to design an ideal protein, resulting in a potential amino acid sequence and structure prototype that is ideally adjusted to the requirements of the targeted animal, and is theoretically completely digestible. Both tools can open up new opportunities to form an integrated framework for precise amino acid nutrition in broilers, helping us to achieve more efficient, resilient, and sustainable production. This information can help to determine the exact ratio of amino acids that will improve the efficiency of the use of nitrogen by poultry.

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“…In consequence, PI is always an interdisciplinary job where team members from the different disciplines need to cooperate from the beginning onwards. Finally, productivity of bioprocesses is often far from ideal to meet the large-scale need for food, drugs, biofuels and bio-based chemicals (Boodhoo et al, 2022). Due to the drastic improvements in process performances by applying PI, these limitations could be solved in the future.…”

Section: Introductionmentioning

confidence: 99%

“…This is based on the fact that in one study of 547 biocatalysis based patents filed between 2000 and 2015 more than two third were based on oxidoreductases (Buller et al, 2018). In addition to the PI approaches discussed herein, there are a wide number of additional approaches, see e.g., (Lye and Woodley, 1999;Bolivar et al, 2011;Wohlgemuth et al, 2015;Lorente-Arevalo et al, 2021;van der Wielen et al, 2021;Žnidaršič-Plazl, 2021;Boodhoo et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Process Intensification as Game Changer in Enzyme Catalysis

et al. 2022

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Enzyme catalysis, made tremendous progress over the last years in identification of new enzymes and new enzymatic reactivity’s as well as optimization of existing enzymes. However, the performance of the resulting processes is often still limited, e.g., in regard of productivity, realized product concentrations and the stability of the enzymes. Different topics (like limited specific activity, unfavourable kinetics or limited enzyme stability) can be addressed via enzyme engineering. On the other hand, there is also a long list of topics that are not addressable by enzyme engineering. Here typical examples are unfavourable reaction thermodynamics, selectivity in multistep reactions or low water solubility. These challenges can only be addressed through an adaption of the reaction system. The procedures of process intensification (PI) represent a good approach to reach most suitable systems. The general objective of PI is to achieve significant benefits in terms of capital and operating costs as well as product quality, waste, and process safety by applying innovative principles. The aim of the review is to show the current capabilities and future potentials of PI in enzyme catalysis focused on enzymes of the class of oxidoreductases. The focus of the paper is on alternative methods of energy input, innovative reactor concepts and reaction media with improved properties.

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Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

Cited by 62 publications

References 250 publications

Complexity reduction and opportunities in the design, integration and intensification of biocatalytic processes for metabolite synthesis

Complexity reduction and opportunities in the design, integration and intensification of biocatalytic processes for metabolite synthesis

Biomarkers and De Novo Protein Design Can Improve Precise Amino Acid Nutrition in Broilers

Process Intensification as Game Changer in Enzyme Catalysis

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