2021
DOI: 10.21203/rs.3.rs-268282/v1
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Bioprocess optimisation for high cell density endoinulinase production from recombinant Aspergillus niger

Abstract: Endoinulinases gene was expressed in recombinant Aspergillus niger for selective and high-level expression using an exponential fed-batch fermentation. The effects of the growth rate (µ), glucose feed concentration, nitrogen concentration and fungal morphology, on enzyme production were evaluated. A recombinant endoinulinases with a molecular weight of 66 KDa was secreted. Endoinulinases production was growth associated at µ> 0.04 h -1 , which is characteristic of the constitutive gpd promoter used for … Show more

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