Bioprocessing of Endophytes for Production of High-Value Biochemicals

Mohinudeen, Khwajah; Devan, Karthik; Srivastava, Smita

doi:10.1007/978-981-13-5862-3_17

Cited by 2 publications

(3 citation statements)

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“…Abdel-Wareth et al [10] reported that ethyl acetate and acetone extracts from M. anisopliae possessed antioxidant activity when evaluated by the phosphomolybdenum method. The lack of antioxidant activity of our fungal strains may be due to various factors such as culture conditions, type of extraction (biomass or supernatant), solvent used [41], or the absence of genes involved in the production of antioxidant compounds [5]. Strains of the same species may differ in their potential to produce secondary metabolites, which is the reason to evaluate different culture conditions to increase the performance and biological activity of fungal strains [42].…”

Section: Discussionmentioning

confidence: 99%

“…Various classes of metabolites with immunomodulatory, antimicrobial, anticancer, and antioxidant activities have been reported with the potential to be biotechnologically exploited [4]. However, for the production of secondary metabolites from endophytic fungi to be economically viable, it is necessary to increase the biological activity of the fungal strains as well as the yield of bioactive compounds [5].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Culture Conditions of Lophocereus marginatus Endophytic Fungi on Yield and Anticancer and Antioxidant Activities

Ramírez-Villalobos

Gómez-Flores

Velázquez-Flores

et al. 2023

IJERPH

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Culture conditions affect the production of secondary metabolites in endophytic fungi. Therefore, the aim of the present study was to evaluate the yield and anticancer and antioxidant activity of endophytic fungi extracts from the cactus Lophocereus marginatus, under different culture conditions. The strains Penicillium citrinum, Aspergillus versicolor, Metarhizium anisopliae, and Cladosporium sp. were fermented in different culture media (potato dextrose agar, Czapeck broth, and malt broth), types of inoculums (spore or mycelium), and shaking conditions (150 rpm or static) for one week. Methanol extracts were obtained from mycelia, which was followed by determining their yields and evaluating their effect on L5178Y-R murine lymphoma cells growth and human peripheral blood mononuclear cells (PBMCs) viability, using the 3-[4,5dimethylthiazol-2-yl]2,5-diphenyl tetrazolium bromide reduction colorimetric assay. In addition, antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl test. We determined the half-maximal inhibitory concentration (IC50) values of tumor cell growth inhibition, the selectivity index (SI), and the antioxidant activity, as compared with the healthy cells control. The best yields were obtained with the CK medium in all the evaluated strains, reaching values of 50.3%. Of the 48 extracts evaluated, only seven significantly (p < 0.01) inhibited tumor cell growth (IC50 < 250 µg/mL). A. versicolor extract showed the highest anticancer activity, after culturing spores (IC50 = 49.62 µg/mL; SI = 15.8) or mycelium (IC50 = 69.67 µg/mL; SI = 12.2) in malt broth, under static conditions. Extracts did not present significant antioxidant activity. In conclusion, we showed that culture conditions influenced the anticancer activity of L. marginatus endophytic fungi.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Culture Conditions of Lophocereus marginatus Endophytic Fungi on Yield and Anticancer and Antioxidant Activities

Ramírez-Villalobos

Gómez-Flores

Velázquez-Flores

et al. 2023

IJERPH

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“…Recovery of endophytic fungi from healthy plant tissues led to a massive argument whether these entophytes are plant pathogen or not. According to Chen et al (2010) and Mohinudeen et al (2019), endophytic fungi may appear at two phases, non-pathogenic and pathogenic. Many non-pathogenic endophytes are dormant pathogens and may become pathogenic under certain environmental stress conditions or after plant aging.…”

Section: Endophytic Fungi As New Sources For Ache Inhibitorsmentioning

confidence: 99%

Microbial acetylcholinesterase inhibitors for Alzheimer’s therapy: recent trends on extraction, detection, irradiation-assisted production improvement and nano-structured drug delivery

Zaki

El‐Sayed

Elkodous

et al. 2020

Appl Microbiol Biotechnol

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Neurodegenerative disorders especially Alzheimer's disease (AD) are significantly threatening the public health. Acetylcholinesterase (AChE) inhibitors are compounds of great interest which can be used as effective agents for the symptomatic treatment of AD. Although plants are considered the largest source for these types of inhibitors, the microbial production of AChE inhibitors represents an efficient, easily manipulated, eco-friendly, cost-effective, and alternative approach. This review highlights the recent advances on the microbial production of AChE inhibitors and summarizes all the previously reported successful studies on isolation, screening, extraction, and detecting methodologies of AChE inhibitors from the microbial fermentation, from the earliest trials to the most promising anti-AD drug, huperzine A (HupA). In addition, improvement strategies for maximizing the industrial production of AChE inhibitors by microbes will be discussed. Finally, the promising applications of nano-material-based drug delivery systems for natural AChE inhibitor (HupA) will also be summarized. Key Points • AChE inhibitors are potential therapies for Alzheimer's disease. • Microorganisms as alternate sources for prospective production of such inhibitors. • Research advances on extraction, detection, and strategies for production improvement. • Nanotechnology-based approaches for an effective drug delivery for Alzheimer's disease.

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Bioprocessing of Endophytes for Production of High-Value Biochemicals

Cited by 2 publications

References 149 publications

Effect of Culture Conditions of Lophocereus marginatus Endophytic Fungi on Yield and Anticancer and Antioxidant Activities

Effect of Culture Conditions of Lophocereus marginatus Endophytic Fungi on Yield and Anticancer and Antioxidant Activities

Microbial acetylcholinesterase inhibitors for Alzheimer’s therapy: recent trends on extraction, detection, irradiation-assisted production improvement and nano-structured drug delivery

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