Bioprocessing technology of muscle stem cells: implications for cultured meat

Vitamin C enhances the ex vivo proliferation of porcine muscle stem cells for cultured meat production

“…5,6 Cultured meat, also known as lab-grown meat or clean meat, is produced ex vivo by culturing animal cells and constructing muscle tissues on a large scale. 7,8 cultured meat has been considered as a promising supplement to animal-based meat in the future. 9–11…”

“…5,6 Cultured meat, also known as lab-grown meat or clean meat, is produced ex vivo by culturing animal cells and constructing muscle tissues on a large scale. 7,8 cultured meat has been considered as a promising supplement to animalbased meat in the future. [9][10][11] The production process of cultured meat can be briefly divided into four steps: (1) isolation of seed cells, (2) scalable expansion of seed cells, (3) generation of muscle fibers via differentiation, and (4) assembly or food processing.…”

“…[9][10][11] The production process of cultured meat can be briefly divided into four steps: (1) isolation of seed cells, (2) scalable expansion of seed cells, (3) generation of muscle fibers via differentiation, and (4) assembly or food processing. 8,12 Although various seed cell types have been proposed, muscle stem cells (MuSCs) are one of the optimal candidates because of their intrinsic myogenic properties. However, it has been widely reported that the ex vivo proliferative capacity of MuSCs is limited, so after a few days of culture, the cell growth rate and myogenic differentiation potential decrease significantly, resulting in the insufficient number of myoblasts for cultured meat production.…”

IGF-1 Signaling Regulates Mitochondrial Remodeling during Myogenic Differentiation

“…Muscle regeneration can be described as a highly coordinated process that begins with activation and proliferation of myoblasts, and functional expression of myogenic regulatory factors, to the fusion of mononuclear myoblasts, ultimately forming multinuclear and contractive muscle fibers [ 5 , 6 ]. Increasing reports and reviews have emphasized mitochondria to be positively involved in the muscle regeneration process [ 7 , 8 ].…”

Bringing the next biotechnologies to life

“…This format will explore technologies from the perspective of the people bringing them into the world, to complement our long-standing coverage from the perspective of the people developing them in the lab. Our first Industry Highlight, in this issue, showcases the approach that Aleph Farms (https://www.alephfarms.com/) takes to develop cultured meat [4,5]. Other planned features in this series cover the wide breadth of applied biology, from natural product discovery to bioprinting and metabolic engineering.…”