Bioproduction and Optimization of Rosmarinic Acid Production in Solenostemon Scutellarioides through Media Manipulation and Conservation of High Yielding Clone via Encapsulation

Abstract

The present study describes the role of different exogenous phytohormones, polyamines and sucrose on growth and rosmarinic acid (RA) production in whole plant culture of Solenostemon scutellarioides. It was further aimed to conserve elite clones via synthetic seed technology. S. scutellarioides was treated either singly or in combination with different phytohormones. Cultures incubated with NAA (0.5 mg L-1) yielded the highest RA accumulation (g-1FW), but negatively affected the growth. So, overall RA content was insignificant. Cultures incubated with IBA, BAP and GA 3 at low concentration significantly improved growth and RA bioaccumulation. In the combinatorial study, IBA+BAP+GA 3 (0.5 mg L-1 each) was found optimum for plant biomass and RA production (65.2% improvement of total RA). Amongst polyamines, putrescine (1 mg L-1) exhibited 20.4% improvement of total RA content. The intracellular RA accumulation (g-1FW) was significantly higher between 5 and 7% of sucrose concentrations. However, the total increase in RA content was inhibited due to deterioration of the culture with increasing sucrose concentration. Based on the effect of different treatments on growth and RA accumulation, a high yielding and stable plant line was selected for conservation via alginate encapsulation. Uniform shaped alginate coated synthetic seeds conserved up to 6 months exhibited high regeneration potential and RA content.

Recent Advances in the Analysis of Rosmarinic Acid From Herbs in the Lamiaceae Family

“…2,3 Lamiaceae species have been used in traditional medicine for the treatment of depression, headache, asthma, 4 stress, 5 and respiratory 6 and Alzheimer's diseases. 7,8 In addition, these plants have antioxidant, 9 antiallergic, 10 antimicrobial, 11 anticarcinogenic, 12 anti-inflammatory, 13 astringent, 14 and antiHIV-1 effects. 15 These properties are mainly due to phenolic compounds found in the plants, especially rosmarinic acid (RA).…”

The influence of cultivation conditions on the formation of psychoactive salvinorin A, salvinorin B, rosmarinic acid and caffeic acid in Coleus scutellarioides

“…Increasing the biosynthesis of rosmarinic acid in Coleus scutellarioides has been an object of investigations undertaken by numerous research teams. Application of the combination IBA (indolylbutyric acid), BAP (6-benzylaminopurine) and GA 3 (gibberellic acid) (0.5 mg∙ dm –3 ) induced the highest efficiency of the rosmarinic acid production, amounting to 65.2% in in vitro cultures of whole plants 24 . Improvement in the biosynthesis of rosmarinic acid under in vitro plant cultures has been attained in the common sage Salvia officinalis L. via supplementation of the breeding medium with phytohormones: in these experiments IAA (indole-3-acetic acid) combined with BAP (6-benzylaminopurine) was added 25 .…”