Bioproduction of D-psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

Abstract

D-psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of D-psicose from D-fructose. In this paper, another bacterium which could convert D-fructose to D-psicose was isolated and identified as Rhodobacter sphaeroides. The process parameters of D-psicose production using permeabilized cells of Rhodobacter sphaeroides SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10 min, and reaction conditions: cell concentration, 30 g dry cell wt/L; concentration of substrate, 50 g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5 g/L D-psicose with a Dpsicose productivity of 0.82 g$L -1 $h -1 . This is the first report of bioproduction of D-psicose using permeabilized cells of Rhodobacter sphaeroides.

D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii

“…The permeabilized cells of wild-type R . sphaeroides treated with 0.1% (w/v) CTAB produced 6.5 g/L d -allulose from 50 g/L d -fructose after 8 h, a specific productivity of 0.03 g/g/h, and a volumetric productivity of 0.81 g/L/h [ 18 ]. The permeabilized cells of wild-type Sinorhizobium sp.…”

“…[ 16 ], and Treponema primitia [ 17 ]; whole cells of R . sphaeroides [ 18 ] and Sinorhizobium sp. [ 19 ]; and whole recombinant cells of Escherichia coli expressing DAE ases from C .…”

“…Permeabilized cells treated with detergents such as cetyl trimethylammonium bromide (CTAB), Tween 20, Tween 80, and Triton X-100 [ 18 , 25 ]; solvents such as acetone, chloroform, ethanol, methanol, and toluene [ 19 , 26 , 27 ]; salts such as NaCl and MgCl 2 [ 28 ]; and chemicals such as dimercaptosuccinic acid, EDTA, and polyethylenimine [ 28 ] have been used in whole-cell bioprocesses because they increase the production of metabolites by enhancing the transfer of reaction substrates and products across the cell membrane [ 26 , 29 ]. Although penicillin has been used in glutamic acid fermentation to permeabilize C .…”

Review on D-Allulose: In vivo Metabolism, Catalytic Mechanism, Engineering Strain Construction, Bio-Production Technology

“…(Zhang et al, 2013b). More DTEases sources and more efficient DTEases activity need to be further explored (Oh et al, 2007;Zhang et al, 2009a;Mu et al, 2011;Jia et al, 2014;Park et al, 2016a,b).…”

“…Both anion exchange resin matrix and synchronous moving bed chromatography are used to immobilize DAEase for D-allulose production from D-fructose. The cells of R. sphaeroides SK011 produces 6.5 g L −1 D-allulose with a productivity of 0.82 g −1 h −1 from initial 50 g L −1 D-fructose by using the dialysis method based on ion exchange resin (Zhang et al, 2009a). The toluene-treated Sinorhizobium sp.…”