Bioproduction of p-hydroxybenzoate from renewable feedstock by solvent-tolerant Pseudomonas putida S12

Verhoef, Suzanne; Ruijssenaars, Harald J.; Bont, J.A.M. de; Wery, Jan

doi:10.1016/j.jbiotec.2007.08.031

Cited by 88 publications

(89 citation statements)

References 27 publications

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“…An alternative to this approach is to identify alternative production hosts that are naturally tolerant to higher concentrations of the chemicals of interest. P. putida is one such strain that is often utilized for bioremediation and production of more toxic chemicals (Loeschcke & Thies, 2015; Nikel, Martínez‐García, & de Lorenzo, 2014; Poblete‐Castro, Becker, Dohnt, dos Santos, & Wittmann, 2012; Verhoef, Ruijssenaars, de Bont, & Wery, 2007; Verhoef, Wierckx, Westerhof, De Winde, & Ruijssenaars, 2009). …”

Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Calero

Jensen

Bojanovič

et al. 2017

Biotech & Bioengineering

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The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P. putida KT2440 toward eleven toxic chemical compounds was investigated. P. putida was found to be significantly more tolerant toward three of the eleven compounds when compared to Escherichia coli. Increased tolerance was for example found toward p‐coumaric acid, an interesting precursor for polymerization with a significant industrial relevance. The tolerance mechanism was therefore investigated using the genome‐wide approach, Tn‐seq. Libraries containing a large number of miniTn5‐Km transposon insertion mutants were grown in the presence and absence of p‐coumaric acid, and the enrichment or depletion of mutants was quantified by high‐throughput sequencing. Several genes, including the ABC transporter Ttg2ABC and the cytochrome c maturation system (ccm), were identified to play an important role in the tolerance toward p‐coumaric acid of this bacterium. Most of the identified genes were involved in membrane stability, suggesting that tolerance toward p‐coumaric acid is related to transport and membrane integrity.

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Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Calero

Jensen

Bojanovič

et al. 2017

Biotech & Bioengineering

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“…However, since the pentose sugar fraction may account for up to 25% of lignocellulosic biomass (12), it is essential that this fraction is utilized efficiently to obtain costeffective biochemical production. In a previous study, the solvent-tolerant bacterium Pseudomonas putida S12, known for its use as a platform host for the production of aromatic compounds (15,16,19,22), was engineered to use D-xylose as a sole carbon source. This was achieved by introducing genes encoding the phosphorylative D-xylose metabolic pathway of Escherichia coli, followed by laboratory evolution (14).…”

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Establishment of Oxidative d -Xylose Metabolism in Pseudomonas putida S12

Meijnen

Winde

Ruijssenaars

2009

Appl Environ Microbiol

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The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose ؊1 ) and a maximum growth rate of 0.21 h ؊1 . Remarkably, most of the genes of the xylXABCD operon appeared to be dispensable for growth on D-xylose. Only the xylD gene, encoding D-xylonate dehydratase, proved to be essential for establishing an oxidative D-xylose catabolic pathway in P. putida S12. The growth performance on D-xylose was, however, greatly improved by coexpression of xylXA, encoding 2-keto-3-deoxy-Dxylonate dehydratase and ␣-ketoglutaric semialdehyde dehydrogenase, respectively. The endogenous periplasmic glucose dehydrogenase (Gcd) of P. putida S12 was found to play a key role in efficient oxidative D-xylose utilization. Gcd activity not only contributes to D-xylose oxidation but also prevents the intracellular accumulation of toxic catabolic intermediates which delays or even eliminates growth on D-xylose.

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“…Solvent-tolerant bacteria are especially useful for the biosynthesis of compounds that are toxic to other microorganisms (7,39,48). They have been used to produce different aromatic compounds, such as cinnamic acid (24), p-hydroxybenzoic acid (33,43), 3-methylcatechol (35, 50), and phenol (51).…”

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“…This strain has since been converted into an efficient producer of other, value-added aromatics (43). Efficient phenol production was achieved by introduction of the tpl gene from Pantoea agglomerans, encoding the enzyme tyrosine-phenol lyase (TPL), followed by a combined approach of targeted genetic engineering, random mutagenesis, antimetabolite selection, and high-throughput screening.…”

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Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production

et al. 2008

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The unknown genetic basis for improved phenol production by a recombinant Pseudomonas putida S12 derivative bearing the tpl (tyrosine-phenol lyase) gene was investigated via comparative transcriptomics, nucleotide sequence analysis, and targeted gene disruption. We show upregulation of tyrosine biosynthetic genes and possibly decreased biosynthesis of tryptophan caused by a mutation in the trpE gene as the genetic basis for the enhanced phenol production. In addition, several genes in degradation routes connected to the tyrosine biosynthetic pathway were upregulated. This either may be a side effect that negatively affects phenol production or may point to intracellular accumulation of tyrosine or its intermediates. A number of genes identified by the transcriptome analysis were selected for targeted disruption in P. putida S12TPL3. Physiological and biochemical examination of P. putida S12TPL3 and these mutants led to the conclusion that the metabolic flux toward tyrosine in P. putida S12TPL3 was improved to such an extent that the heterologous tyrosine-phenol lyase enzyme had become the rate-limiting step in phenol biosynthesis.Pseudomonas putida is a ubiquitous soil bacterium that has become increasingly important for a wide range of biotechnological applications (46). Its broad biocatalytic potential makes it highly suitable for applications such as bioremediation (8) and biocatalysis (7,40). Of special interest are solvent-tolerant P. putida strains. These organisms have evolved several mechanisms to deal with toxic compounds, including modifications of the inner and outer membranes and active extrusion of a broad range of compounds through membrane-associated efflux pumps (18,31,32). Solvent-tolerant bacteria are especially useful for the biosynthesis of compounds that are toxic to other microorganisms (7,39,48). They have been used to produce different aromatic compounds, such as cinnamic acid (24), p-hydroxybenzoic acid (33, 43), 3-methylcatechol (35, 50), and phenol (51).Previously, we reported the construction of a P. putida S12 strain which efficiently produces phenol from glucose as a demonstration case for the "green" production of a toxic, hydroxylated aromatic compound (51). This strain has since been converted into an efficient producer of other, value-added aromatics (43). Efficient phenol production was achieved by introduction of the tpl gene from Pantoea agglomerans, encoding the enzyme tyrosine-phenol lyase (TPL), followed by a combined approach of targeted genetic engineering, random mutagenesis, antimetabolite selection, and high-throughput screening. This approach resulted in strain P. putida S12TPL3, which was capable of converting glucose into phenol with a yield of 7% (mol/mol) (51). The optimization of phenol production was achieved mostly by random approaches. As a result, little is known about the genetic basis of the enhanced phenol production.Transcriptome analysis has been used successfully in the past to gain comprehensive insight into complex metabolic networks (12,27,29) and o...

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Bioproduction of p-hydroxybenzoate from renewable feedstock by solvent-tolerant Pseudomonas putida S12

Cited by 88 publications

References 27 publications

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Establishment of Oxidative d -Xylose Metabolism in Pseudomonas putida S12

Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production

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