Bioproduction strategies for rare hexose sugars

Izumori, Ken

doi:10.1007/s00114-002-0297-z

Cited by 193 publications

(114 citation statements)

References 24 publications

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“…3A). D-Psicose is considered an ultralow-energy "rare sugar," found in nature only in small quantities in agricultural products and commercially prepared carbohydrate complexes (11). Prior to this communication, B. subtilis had not been reported to utilize D-psicose.…”

Section: Resultsmentioning

confidence: 99%

Uncovering New Metabolic Capabilities of Bacillus subtilis Using Phenotype Profiling of Rifampin-Resistant rpoB Mutants

Perkins

Nicholson

2008

J Bacteriol

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A central goal of the genomics-bioinformatics era is to understand at a global level how cells coordinate the conversion of their genomic information into phenotype. From this perspective, macromolecular machines such as the replication, transcription, and translation complexes can be viewed as central control devices that optimize the flow of genetic information to the various cellular tasks needed for sensing and responding to environmental cues. It has been well established for the gram-positive spore-forming bacterium Bacillus subtilis that growth and metabolism, as well as several developmental events (e.g., extracellular enzyme synthesis, motility, chemotaxis, competence, sporulation, spore resistance properties, germination, and outgrowth), are controlled in large part at the transcriptional level (26,27,28). Because the RNA polymerase transcription complex contacts every promoter in the B. subtilis genome, single amino acid substitutions in critical portions of the enzyme can lead to global changes in gene transcription and hence in physiology and metabolism.One such critical target resides on the ␤ subunit of RNA polymerase in the binding site for the antibiotic rifampin (Rif). Rifampin resistance (Rif r ) results from mutations in the rpoB gene leading to specific amino acid alterations in ␤ (12; reviewed in reference 37). Evidence connecting RNA polymerase, Rif r , and global regulation of growth and development in B. subtilis has been accumulating. (i) Rothstein et al. (31) first isolated a Rif r B. subtilis mutant exhibiting a temperature-sensitive sporulation defect; the mutation responsible, rfm2103, was later identified as causing the amino acid change H482Y in cluster I of ␤ (7).(ii) Rif r mutations mapping to cluster I (Q469K, Q469R, and H482Y) affected the efficiency of rho-dependent transcription termination (10). (iii) Rif r mutations in cluster I, especially S487L, were reported to enhance postexponential production of the extracellular enzymes amylase and protease (S. T. Jørgensen, unpublished data; see also reference 9a). (iv) We observed that four Rif r mutations in cluster I (Q469R, H482R, H482Y, and S487L) caused both locus-specific and allele-specific alterations in the expression of global transcriptional regulons controlling growth, competence, sporulation, and germination (19). Collectively these observations suggest that alteration of single amino acids in the Rif-binding pocket of ␤ results in fundamental alterations in the global interactions of RNA polymerase with promoters and transcriptional regulators, leading to profound changes in global phenotypes.Using B. subtilis as a model organism, we have been exploring the connections linking environmental selective pressure, mutagenesis, altered phenotype, and fitness during evolution (18). We found that during long-term laboratory evolution of B. subtilis the rate of spontaneous mutation to Rif r increased in evolving cultures by 1 to 2 orders of magnitude over the ancestral mutation rate (18), and we have noted that differing cel...

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Section: Resultsmentioning

confidence: 99%

Uncovering New Metabolic Capabilities of Bacillus subtilis Using Phenotype Profiling of Rifampin-Resistant rpoB Mutants

Perkins

Nicholson

2008

J Bacteriol

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“…Both d-allose and d-glucose are monosaccharides, and d-allose is a C-3 epimer of d-glucose with 80% of the sweetness of sucrose. d-allose is one of many rare sugars that exist in very small quantities in nature (Izumori 2002), and our group has reported its unique properties such as anti-inflammatory (Gao et al 2013), anti-oxidative (Ishihara et al 2011), and inhibitory effects on osteoclast differentiation (Yamada et al 2012). d-allose inhibits the growth of many types of cancer cells, such as human hepatocellular carcinoma (HuH-7), HepG2, human gastric cancer (OVCAR3), and HeLa cells (Sui et al 2005a, b) and reduces the tumor volume in in vivo experiments (Hoshikawa et al 2010).…”

Section: Introductionmentioning

confidence: 99%

D-Allose Inhibits Cancer Cell Growth by Reducing GLUT1 Expression

Noguchi

Kamitori

Hossain

et al. 2016

Tohoku J. Exp. Med.

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Glucose is a major energy source for mammalian cells and is transported into cells via cell-specific expression of various glucose transporters (GLUTs). Especially, cancer cells require massive amounts of glucose as an energy source for their dysregulated growth and thus over-express GLUTs. d-allose, a C-3 epimer of d-glucose, is one of rare sugars that exist in small quantities in nature. We have shown that d-allose induces the tumor suppressor gene coding for thioredoxin interacting protein (TXNIP) and inhibits cancer cell growth by G1 cell cycle arrest. It has also been reported that GLUTs including GLUT1 are overexpressed in many cancer cell lines, which may contribute to larger glucose utilization. Since d-allose suppresses the growth of cancer cells through the upregulation of TXNIP expression, our present study focused on whether d-allose down-regulates GLUT1 expression via TXNIP expression and thus suppresses cancer cell growth. Western blot and real-time PCR analyses revealed that d-allose significantly induced TXNIP expression and inhibited GLUT1 expression in a dose-dependent manner in three human cancer cell lines: hepatocellular carcinoma (HuH-7), Caucasian breast adenocarcinoma (MDA-MB-231), and neuroblastoma (SH-SY5Y). In these cell lines, d-allose treatment inhibited cell growth. Importantly, d-allose treatment decreased glucose uptake, as measured by the uptake of 2-deoxy d-glucose. Moreover, the reporter assays showed that d-allose decreased the expression of luciferase through the hypoxia response element present in the tested promoter region. These results suggest that d-allose may cause the inhibition of cancer growth by reducing both GLUT1 expression and glucose uptake.

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“…Fax: +81-87-891-3021; E-mail: s99x603@stmail.ag.kagawa-u.ac.jp the name Izumoring. 8,9) According to this ring, the production of all hexoses from inexpensive monosaccharides such as Glc and D-fructose (Fru) has been studied using microbial and enzymatic reactions. A method of production of D-altrose (Alt) and D-allose (All) from D-psicose (Psi) using immobilized L-rhamnose isomerase has been established, 10) and also one for mass production of Psi from Fru using an immobilized D-tagatose (Tag) 3-epimerase bioreactor.…”

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confidence: 99%