Bioprospecção de genes biossintéticos de policetídeos em DNA metagenômico de solo de Mata Atlântica.

Massini, Karen Cristina

doi:10.11606/t.42.2009.tde-29012010-111747

Cited by 2 publications

(2 citation statements)

References 104 publications

(63 reference statements)

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“…, kit Ultra Clean Mega Soil DNA from Mo Bio) and even diversified non-commercial extraction methods (Liles et al , 2008) that, in part, allow for the circumvention of these difficulties; however, the physical-chemical nature of the environment directly influences their performance, requiring adjustments for each condition studied (Massini, 2009; Schuch, 2007). …”

Section: Metagenomics As a Tool For Discovery Of Novel Polyketidesmentioning

confidence: 99%

Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomics

Gomes¹,

Schuch²,

Lemos³

2013

Braz. J. Microbiol.

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The discovery of secondary metabolites produced by microorganisms (e.g., penicillin in 1928) and the beginning of their industrial application (1940) opened new doors to what has been the main medication source for the treatment of infectious diseases and tumors. In fact, approximately 80 years after the discovery of the first antibiotic compound, and despite all of the warnings about the failure of the “goose that laid the golden egg,” the potential of this wealth is still inexorable: simply adjust the focus from “micro” to “nano”, that means changing the look from microorganisms to nanograms of DNA. Then, the search for new drugs, driven by genetic engineering combined with metagenomic strategies, shows us a way to bypass the barriers imposed by methodologies limited to isolation and culturing. However, we are far from solving the problem of supplying new molecules that are effective against the plasticity of multi- or pan-drug-resistant pathogens. Although the first advances in genetic engineering date back to 1990, there is still a lack of high-throughput methods to speed up the screening of new genes and design new molecules by recombination of pathways. In addition, it is necessary an increase in the variety of heterologous hosts and improvements throughout the full drug discovery pipeline. Among numerous studies focused on this subject, those on polyketide antibiotics stand out for the large technical-scientific efforts that established novel solutions for the transfer/engineering of major metabolic pathways using transposons and other episomes, overcoming one of the main methodological constraints for the heterologous expression of major pathways. In silico prediction analysis of three-dimensional enzymatic structures and advances in sequencing technologies have expanded access to the metabolic potential of microorganisms.

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Section: Metagenomics As a Tool For Discovery Of Novel Polyketidesmentioning

confidence: 99%

Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomics

Gomes¹,

Schuch²,

Lemos³

2013

Braz. J. Microbiol.

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“…O metabolismo secundário é interessante devido à produção de uma variedade de compostos que são de grande utilidade como: antibióticos, antifúngicos, agentes imunossupressores, antitumoral, inibidores de enzimas, toxinas e pigmentos. A maioria são moléculas orgânicas complexas produzidas por uma cadeia de reações enzimáticas (DEMAIN, 1992;KLEINKAUF;VON DÖHREN, 1996;MASSINI, 2009 In prokaryotes, iterative type II PKS systems are much more common, and also restricted to these organisms. In these systems, a minimal set of iteratively used enzymes, each expressed from a distinct gene, is required for polyketide ate.…”

Section: Metabólitos Secundáriosunclassified

Caracterização dos mecanismos de resistência ao antibiótico antitumoral cosmomicina D.

Arteaga¹

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Bioprospecção de genes biossintéticos de policetídeos em DNA metagenômico de solo de Mata Atlântica.

Cited by 2 publications

References 104 publications

Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomics

Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomics

Caracterização dos mecanismos de resistência ao antibiótico antitumoral cosmomicina D.

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