Bioprospecting for native microalgae as an alternative source of sugars for the production of bioethanol

Rizza, Lara Sánchez; Smachetti, María Eugenia Sanz; Nascimento, Mauro Do; Salerno, Graciela L.; Curatti, Leonardo

doi:10.1016/j.algal.2016.12.021

Cited by 122 publications

(44 citation statements)

References 41 publications

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“…The observed total carbohydrates to ethanol conversion was very close to the theoretical maximum conversion yield of 0.51 g ethanol per g of glucose and a biomass to ethanol conversion efficiency of 0.25 g ethanol per g biomass. The corresponding amount of CO 2 release and the production of low amounts of yeast biomass were confirmed, as reported before (Sanchez Rizza et al, 2017). We showed a large improvement of ethanol yields from Desmodesmus sp.…”

Section: Saccharification Of Microalgal Biomass and Ethanol Productionsupporting

confidence: 89%

“…strain FG biomass compared with previous work in microalgal biomass transformation into ethanol (Sanchez Rizza et al, 2017). Here, we further improved sugars and ethanol concentration by about 2-fold by increasing the biomass load during diluted acid saccharification from 10 to 20 % (w/v), with no signs of inhibition of fermentation yet.…”

Section: Saccharification Of Microalgal Biomass and Ethanol Productionmentioning

confidence: 60%

“…For crude fat determinations, dry and milled samples were extracted with petroleum ether in an Ankom XT10 equipment. Water soluble carbohydrates were extracted in a boiling aqueous solution, filtered, and determined by the anthrone reagent as described above.Ethanol was determined from the S. cerevisiae fermentation spent-medium by an enzymatic assay as reported previously(Sanchez Rizza et al, 2017). Briefly, the standard ethanol assays contained 50 mM Tris-HCl, pH 8.4; 2.5 mM NAD + and 3 µg protein preparations enriched in alcohol dehydrogenase activity.…”

mentioning

confidence: 99%

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A semi-closed loop microalgal biomass production-platform for ethanol from renewable sources of nitrogen and phosphorous

Rizza

Coronel

Smachetti

et al. 2019

Journal of Cleaner Production

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Section: Saccharification Of Microalgal Biomass and Ethanol Productionsupporting

confidence: 89%

Section: Saccharification Of Microalgal Biomass and Ethanol Productionmentioning

confidence: 60%

mentioning

confidence: 99%

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A semi-closed loop microalgal biomass production-platform for ethanol from renewable sources of nitrogen and phosphorous

Rizza

Coronel

Smachetti

et al. 2019

Journal of Cleaner Production

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“…While acid hydrolysis, mostly using diluted sulfuric acid, requires high temperatures and produces large volume of contaminating residues, enzymatic hydrolysis can be completed under milder temperatures, although it still requires some physical or chemical pretreatments, and it is considerably more expensive (Hernández et al, 2015). Nevertheless, both strategies lead to biomass saccharification and conversion into ethanol by microbial fermentation with an efficiency very close to the upper theoretical value, as recently compiled by Sanchez Rizza and co-workers (Sanchez Rizza et al, 2017;Sanz Smachetti et al, 2018).…”

Section: Introductionmentioning

confidence: 92%

Ethanol and protein production from minimally processed biomass of a genetically-modified cyanobacterium over-accumulating sucrose

Smachetti

Cenci

Salerno

et al. 2019

Bioresource Technology Reports

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One of the main bottlenecks of a microalgal or cyanobacterial biomass biorefinery is the separation of different useful fractions using simple, low energy-consuming, cost-effective, and scalable separation processes. Although the carbohydrates-rich biomass of these microorganisms presents clear advantages over conventional terrestrial crops as feedstocks for ethanol, it still requires acid and/or enzymatic hydrolysis for efficient fermentation. Here, we show the genetic modification of carbohydrates partitioning in a filamentous cyanobacterium towards the accumulation of sucrose up to 10% (w/w) as a readily fermentable feedstock. We optimized two methods for the preparation of concentrated sucrose syrups, which were efficiently converted into ethanol by yeasts, without the need of additional pretreatments. Biomass drying and milling, followed by aqueous extraction of sugars and proteins, and the recovery of proteins by short pulses of heat, kept the value of sugars as a feedstock for ethanol and protein for feed supplements within a cost-effective biomass biorefinery.

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“…To achieve a positive economic balance, several matters should be taken into account such as CO 2 capture [6,7], water quality improvement [8,9], procurement of commodities [10,11], and high-added value products [12,13].…”

Section: Introductionmentioning

confidence: 99%

Steam Explosion and Vibrating Membrane Filtration to Improve the Processing Cost of Microalgae Cell Disruption and Fractionation

Lorente¹,

Haponska²,

Clavero³

et al. 2018

Processes

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The aim of this study is to explore an innovative downstream route for microalgae processing to reduce cost production. Experiments have been carried out on cell disruption and fractionation stages to recover lipids, sugars, and proteins. Steam explosion and dynamic membrane filtration were used as unit operations. The species tested were Nannochloropsis gaditana, Chlorella sorokiniana, and Dunaliella tertiolecta with different cell wall characteristics. Acid-catalysed steam explosion permitted cell disruption, as well as the hydrolysis of carbohydrates and partial hydrolysis of proteins. This permitted a better access to non-polar solvents for lipid extraction. Dynamic filtration was used to moderate the impact of fouling. Filtration enabled two streams: A permeate containing water and monosaccharides and a low-volume retentate containing the lipids and proteins. The necessary volume of solvent to extract the lipids is thus much lower. An estimation of operational costs of both steam explosion and membrane filtration was performed. The results show that the steam explosion operation cost varies between 0.005 $/kg and 0.014 $/kg of microalgae dry sample, depending on the cost of fuel. Membrane filtration cost in fractionation was estimated at 0.12 $/kg of microalgae dry sample.

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Bioprospecting for native microalgae as an alternative source of sugars for the production of bioethanol

Cited by 122 publications

References 41 publications

A semi-closed loop microalgal biomass production-platform for ethanol from renewable sources of nitrogen and phosphorous

A semi-closed loop microalgal biomass production-platform for ethanol from renewable sources of nitrogen and phosphorous

Ethanol and protein production from minimally processed biomass of a genetically-modified cyanobacterium over-accumulating sucrose

Steam Explosion and Vibrating Membrane Filtration to Improve the Processing Cost of Microalgae Cell Disruption and Fractionation

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