Bioprospecting fungal diversity from crude oil infiltrate soil of Assam, India’s Northeast

Abstract

Abstract:The Screening of native fungi from crude oil contaminated soil of Assam is conducted. Total crude oil concentrations varied from 39000 mg.Kg -1 to 51500 mg.Kg -1 . The soil fungal population varied from 14.6×10 6 CFU.g -1 to 19.2×10 6 CFU.g -1 soil. Twenty fungal strains were isolated and out of them seven were selected on the basis of their ability to grow in PDA medium where crude oil is used as sole carbon source. The growth rate and tolerance of each strain to crude oil were examined and were found quite satisfactory. The radial extension rate varied from 0.03 cm.d −1 to 0.50 cm.d −1 and exhibited significant differences (P< 0.01) among the selected strains.The degradation percentage of crude oil was in the range of 71% to 89% in mineral salt medium (MSL) after the incubation for 28 days showing outstanding potential of isolates.

The Role of Aspergillus parasiticus NRRL:3386 Strain on Petroleum Biodegradation and Biosorption Processes

“…Maddela et al reported that two fungal isolates degraded 79.9% of petroleum in 30 days (17). About 60% of biodegradation of petroleum was performed by different Aspergillus strains (A. flavus, A. fumigatus, A. versicolor, A. terreus) after 7 days of the incubation period and the biodegradation of petroleum was over 80% after 28 days (5). Although an increase in the incubation period usually led to an increase of petroleum biodegradation in different studies, the increase in incubation period did not lead to a significant increase in the removal of petroleum in this study (Figure 1).…”

“…Many microorganisms can use petroleum hydrocarbons as carbon and energy sources and convert them into CO 2 , H 2 O and nontoxic products (4). While the research in literature is limited to bacterial remediation, the usage of potential fungal strains in remediation processes, is very important in the development of new mycoremediation strategies (5). Fungi are highly capable of degrading complex structures and long chain hydrocarbons and are more resistant to the toxic effects of petroleum hydrocarbons than bacteria (4).…”

“…Remediation of oil spills by chemical and physical remediation methods provide an improvement of 40-50%. However, these methods are quite complex, expensive and time consuming and untreated biodegradable particles are still left in the environment (1,5). In this regard, the most effective approach to the remediation of petroleum contaminated areas is the use of biological methods (5).…”

Optimization of Mycoremediation Potential of A Fungi: Aspergillus Ochraceus Strain

“…In addition, these methods are not effective enough, and their applicability to large areas is very low. In this direction, the most promising approach to treating these areas is biological methods (Deshmukh et al 2016;Islam et al 2017). Microbial Enhanced Oil Recovery (MEOR) is a cost-effective and environmentally friendly technology as well as being biologically based (Zhang et al 2016; Asemoloye et al 2020).…”