Bioprospecting marine actinomycetes for antileishmanial drugs: current perspectives and future prospects

Davies-Bolorunduro, Olabisi Flora; Osuolale, Olayinka; Saibu, Salametu; Adeleye, Isaac Adeyemi; Aminah, Nanik Siti

doi:10.1016/j.heliyon.2021.e07710

Cited by 21 publications

(11 citation statements)

References 156 publications

(219 reference statements)

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“…Actinobacteria are also widely distributed throughout marine habitats, which, different from terrestrial habitats, are characterized by highly dynamic pressure, salinity, pH, dissolved oxygen, and light intensity. Marine actinobacteria have been attracting particular attention as new producers of novel antibiotics such as salinosporamide ( Feling et al, 2003 ), ilamycin ( Ma J. et al, 2017 ), and anticancer agents with unusual properties ( Manivasagan et al, 2014a ; Hassan and Shaikh, 2017 ; Davies-Bolorunduro et al, 2021 ). The genus Streptomyces alone accounts for a remarkable 80% of the actinobacterial natural products and, therefore, has unrivaled biosynthetic capacity in the microbial world ( Manivasagan et al, 2014b ; Hu et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Streptomyces marincola sp. nov., a Novel Marine Actinomycete, and Its Biosynthetic Potential of Bioactive Natural Products

et al. 2022

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Marine actinomycetes are an important source of antibiotics, but many of them are yet to be explored in terms of taxonomy, ecology, and functional activity. In this study, two marine actinobacterial strains, designated SCSIO 64649T and SCSIO 03032, were isolated, and the potential for bioactive natural product discovery was evaluated based on genome mining, compound detection, and antimicrobial activity. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain SCSIO 64649T formed a single clade with SCSIO 03032 (similarity 99.5%) and sister clades with the species Streptomyces specialis DSM 41924T (97.1%) and Streptomyces manganisoli MK44T (96.8%). The whole genome size of strain SCSIO 64649T was 6.63 Mbp with a 73.6% G + C content. The average nucleotide identity and digital DNA–DNA hybridization between strain SCSIO 64649T and its closest related species were well below the thresholds recommended for species delineation. Therefore, according to the results of polyphasic taxonomy analysis, the strains SCSIO 64649T and SCSIO 03032 are proposed to represent a novel species named Streptomyces marincola sp. nov. Furthermore, strains SCSIO 64649T and 03032 encode 37 putative biosynthetic gene clusters, and in silico analysis revealed that this new species has a high potential to produce unique natural products, such as a novel polyene polyketide compounds, two mayamycin analogs, and a series of post-translationally modified peptides. In addition, other important bioactive natural products, such as heronamide F, piericidin A1, and spiroindimicin A, were also detected in strain SCSIO 64649T. Finally, this new species’ metabolic crude extract showed a strong antimicrobial activity. Thanks to the integration of all these analyses, this study demonstrates that the novel species Streptomyces marincola has a unique and novel secondary metabolite biosynthetic potential that not only is beneficial to possible marine hosts but that could also be exploited for industrial applications.

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Section: Introductionmentioning

confidence: 99%

Streptomyces marincola sp. nov., a Novel Marine Actinomycete, and Its Biosynthetic Potential of Bioactive Natural Products

et al. 2022

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“…The majority of antibiotics currently used in clinic are derived from microbes [ 9 , 10 ] which are either naturally occurring products or directly derived from such [ 7 ]. Among microbes, actinobacteria in particular Streptomyces , are responsible for suppling two-thirds of all known antibiotics utilized in modern medicine [ 11 , 12 ]. Filamentous actinomycetes produce over 64% of the known NP antibiotic classes [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of endophytic actinobacteria from Citrullus colocynthis (L.) Schrad and their antibacterial properties

et al. 2022

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Background Antibiotic resistance poses a major threat to human health globally. Consequently, new antibiotics are desperately required to discover and develop from unexplored habitats to treat life-threatening infections. Microbial natural products (NP) are still remained as primary sources for the discovery of new antibiotics. Endophytic actinobacteria (EA) which are well-known producers of bioactive compounds could provide novel antibiotic against pathogenic bacteria. This research aimed to isolate EA from the Citrullus colocynthis plant and explore the antibacterial properties of their metabolites against pathogenic bacteria. Results The healthy samples were collected, dissected and surface-sterilized before cultured on four different selection media at 28 °C. Six endophytic actinobacteria were isolated from Citrullus colocynthis plant. They were taxonomically classified into two family namely Streptomycetaceae and Nocardiopsaceae, based on colony morphological features, scanning electron microscope analysis and molecular identification of isolates. This is the first report on the identification of EA form Citrullus colocynthis and their antibacterial activity. The strains generated a chain of vibrio-comma, cubed or cylindrical shaped spores with indenting or smooth surfaces. Three of those were reported as endophytes for the first time. The strain KUMS-C1 showed 98.55% sequence similarity to its closely related strains which constitutes as a novel species/ strain for which the name Nocardiopsis colocynthis sp. was proposed for the isolated strain. Five isolated strains had antagonist activity against S. aureus, P. aeruginosa, and E. coli. Among those, stain KUMS-C6 showed the broadest spectrum of antibacterial activity against all test bacteria, whereas the strain KUMS-C4 had no antibacterial activity. Conclusions NPs have a long history of safe and efficient use for development of pharmaceutical products. Our study highlights that Citrullus colocynthis is an untapped source for the isolation of EA, generating novel and bioactive metabolites by which might lead to discovery of new antibiotic(s). This study reveals the future of new antibiotic developments looks bright against multi-drug resistance diseases by mining under- or unexplored habitats.

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“…With the abundance of sodium in the ocean, it is an essential prerequisite to add sodium chloride (NaCl) for the growth of most marine organisms. Thus, a well-designed growth medium should have osmotic values similar to those of seawater, which allows the efficient growth of marine actinomycetes [ 61 , 80 ]. This means that a growth medium should contain natural sea water, artificial sea water or deionized/distilled water with the addition of different concentrations of sodium chloride [ 46 ].…”

Section: Cultivation Techniques Of Marine Actinomycetesmentioning

confidence: 99%

Marine Actinomycetes Associated with Stony Corals: A Potential Hotspot for Specialized Metabolites

et al. 2022

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Microbial secondary metabolites are an important source of antibiotics currently available for combating drug-resistant pathogens. These important secondary metabolites are produced by various microorganisms, including Actinobacteria. Actinobacteria have a colossal genome with a wide array of genes that code for several bioactive metabolites and enzymes. Numerous studies have reported the isolation and screening of millions of strains of actinomycetes from various habitats for specialized metabolites worldwide. Looking at the extent of the importance of actinomycetes in various fields, corals are highlighted as a potential hotspot for untapped secondary metabolites and new bioactive metabolites. Unfortunately, knowledge about the diversity, distribution and biochemistry of marine actinomycetes compared to hard corals is limited. In this review, we aim to summarize the recent knowledge on the isolation, diversity, distribution and discovery of natural compounds from marine actinomycetes associated with hard corals. A total of 11 new species of actinomycetes, representing nine different families of actinomycetes, were recovered from hard corals during the period from 2007 to 2022. In addition, this study examined a total of 13 new compounds produced by five genera of actinomycetes reported from 2017 to 2022 with antibacterial, antifungal and cytotoxic activities. Coral-derived actinomycetes have different mechanisms of action against their competitors.

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Bioprospecting marine actinomycetes for antileishmanial drugs: current perspectives and future prospects

Cited by 21 publications

References 156 publications

Streptomyces marincola sp. nov., a Novel Marine Actinomycete, and Its Biosynthetic Potential of Bioactive Natural Products

Streptomyces marincola sp. nov., a Novel Marine Actinomycete, and Its Biosynthetic Potential of Bioactive Natural Products

Isolation and identification of endophytic actinobacteria from Citrullus colocynthis (L.) Schrad and their antibacterial properties

Marine Actinomycetes Associated with Stony Corals: A Potential Hotspot for Specialized Metabolites

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