Bioprospecting Micromonospora from Kaziranga National Park of India and their anti-infective potential

Abstract

Large number of strains was isolated from soils of Kaziranga National Park of North-East India using selective isolation procedure. They were assigned to the genus Micromonospora on the basis of their typical colonial and pigmentation features. The taxonomic identities of the isolates were confirmed on the basis of their molecular characters (16SrDNA). A total of one hundred Micromonospora strains were isolated during the present investigation. The diagnostic cell wall sugar and amino acids were determined from these Micromonospora strains. After preliminary screening most of the isolates exhibited excellent anti-infective activity against human bacterial pathogens Staphylococcus aureas, Bacillus subtilis, Proteus vulgaris, Echerichia coli, Pseudomonas aeroginosa and fungal pathogens Aspergillus niger, Fusarium oxysporum and Candida albicans. Among these isolates one strain designated as HK-10 showed promising activity against human pathogens S. aureas, B. subtilis, P. vulgaris and P. aeroginosa.

Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment

“…Investigation of antimicrobial secondary metabolites producing actinobacteria from various ecosystems of Northeast India is reported by a number of researchers (Talukdar et al, 2012; Sharma et al, 2014, 2016; Passari et al, 2015; Zothanpuia et al, 2015). After literature review, Tamdil, a fresh water lake of Mizoram, North east India, which is one of the 115 wetlands in India has been found unexplored ecosystem for actinobacterial research.…”

Structure elucidation and biological activity of antibacterial compound from Micromonospora auratinigra , a soil Actinomycetes

“…The strain HK‐10 was identified using morphological, biochemical and 16SrDNA analysis (Talukdar et al . ).…”

“…The strain M. auratinigra, designated as HK-10 (GenBank accession no. JN381554), was selected in our studies for its promising antibacterial activity against several bacterial test pathogens (Talukdar et al 2012). The strain HK-10 was identified using morphological, biochemical and 16SrDNA analysis (Talukdar et al 2012).…”

“…In our previous investigation (Talukdar et al . ), we had isolated hundreds of Micromonospora strains and most of them possessed antimicrobial potential. Among them, the strain designated as HK‐10 was chosen for further studies for its promising activities.…”

“…The present investigation was, therefore, carried out with an objective to find out an effective antimicrobial molecule from Micromonospora auratinigra, HK-10, collected from the soil of Kaziranga National park of Assam. In our previous investigation (Talukdar et al 2012), we had isolated hundreds of Micromonospora strains and most of them possessed antimicrobial potential. Among them, the strain designated as HK-10 was chosen for further studies for its promising activities.…”