Bioprospecting of clot buster enzyme producing <i>Staphylococcus</i> sp from bovine milk sample

Mohanasrinivasan, V.; Rawat, Gurav; Keziah, Merlin; Anbalagan, Nivetha

doi:10.5958/0974-360x.2018.00335.9

Cited by 3 publications

(3 citation statements)

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“…The comparative results between Phenotypic and genotypic characteristics of staphylokinase were compatible in terms of the presence and absence and also the intensity (strong positive) and the very sharp band had referred the copy number of this gene. Remarkably, figure 3 illustrate that samples (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)18) showed clear obvious thick band while others were less thought they harbored the gene. Jasim and his partners [26] declared in their work that bacteriophages which invade many bacterial species contribute to control the expression of virulence factors and evolutions of pathogenicity which might explain the current results.…”

Section: Resultsmentioning

confidence: 98%

“…The kinase enzyme also has proteolytic acitivity which helps to degrade the fibrin clot, a major constituent of thrombus. The blockage of blood vessels can cause myocardial disorders due to blood clot eventually leading to death [5,6]. Staphylokinase; the single chain protein with 15.5 kDa is considered as a bolus thrombolytic that aids in chasing or partial digesting of the plasminogen to an inactive pro-enzyme; plasmin with high fibrin specificity thus acting as a clot dissolver [7, 8 , 9 ,10].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Sak Gene and Expression of Staphylokinase in Different Clinical Isolates of Staphyloccocus spp

Abed¹,

AL-Azzawie²

2018

Tikrit J. Pure Sci.

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This study had investigated the presence of Staphylokinase (SAK) phenotypically and genetically. A total of 100 Staphylococcus spp. samples were isolated from different clinical cases. Morphological, biochemical tests and for further conformation; Viteck2 technique were adopted to isolate and diagnose the staphylococcal samples. Phenotypic assay tests including hydrolysis of casein and plasma agar were performed to check for the production of Staphylokinase from producing Staphylococcus spp. Out of 100, only 32 isolates had exhibited (SAK) activity whereby 29 of them represented the species S. aureus while 3 belonged to others. All isolates were subjected to Polymerase Chain Reaction (PCR) to determine the possessed sak gene. This investigation certainly had shown that not all isolates of S. aureus has the sak gene, only the lysogenic one has it and that it is not species committed but also other staphylococcus species could have it.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Detection of Sak Gene and Expression of Staphylokinase in Different Clinical Isolates of Staphyloccocus spp

Abed¹,

AL-Azzawie²

2018

Tikrit J. Pure Sci.

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“…Human plasma was separated and heated at 56°C in a water bath for 20min then mixed with melted nutrient agar in ratio 3:1 nutrient agar to plasma (Mohana Srinivasan et al, 2013). A volume of 20μl of the concentrated enzyme was applied into wells cut in the heated plasma agar plates, incubated at 37°C for 24h and checked for a halo zone around wells.…”

Section: Screening Of Staphylokinase Activitymentioning

confidence: 99%

Antibacterial potential of some seaweeds species to combat biofilm-producing multi-drug resistant Staphylococcus aureus of Nile tilapia

et al. 2019

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T HERE is an increasing worldwide demand for seafood due to the awareness of fish as an important protein source for a growing population. However, the diseases caused by multi-drug resistant (MDR) bacterial infections and the low survival rate of the fish represent significant challenges to fish farmers. The virulence and pathogenicity of bacteria are often enhanced when growing as a biofilm. Therefore, a variety of new antimicrobial drugs has attracted wide attention in treating fish pathogen bacteria. Herein, the efficiency of macroalgal extracts as an antibacterial agent against MDR Staphylococcus aureus bacteria isolated from Nile tilapia (Oreochromis niloticus) was studied. Out of 200 bacterial isolates, 50 strains were identified as S. aureus. Of these strains, 37 were MDR and showed a potential role in the production of virulence factors, including staphylokinase (70.2%), lecithinase (81%), protease (56.7%) and lipase (59.4%). Significant production of biofilm virulence factor by MDR S. aureus strains was also observed from the quantitative and qualitative analysis. Four algal species namely Jania rubens, Ulva lactuca, Sargassum vulgare, and Sargassum fusiforme were tested for their antibacterial activity against MDR S. aureus strains. Of those, S. vulgare diethyl ether extract showed the highest antibacterial. In addition, GC-MS analysis revealed 20 identified components in S. vulgare diethyl ether extract, in which Longifolene was dominant (16.5%). This study thus established the possibility of developing an antibacterial agent to combat developing MDR S. aureus and biofilm-related infections in Nile tilapia.

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Molecular Detection of some Staphylococcal species with the newly developed folp gene sequences

El‐Sokkary

2022

RJPT

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16SrRNA gene sequencing, universally accepted for bacterial identification could not effectively discriminate some closely related Staphylococcal species. For this reason, for bacterial species identification, other DNA sequencing genes are required. In this study, folp gene, encoding DHPS, was tested as a possible and alternative phylogenetic marker for more closely related Staphylococcal species. In this new method, 813 bp were implemented for classification instead of 1447 of 16S rRNA mostly used. Phylogenetic analysis was performed based on DNA sequences obtained from the GenBank including most important Staphylococcal species. As a result, the 16S rRNA-based tree clearly delineated 7 distinct clusters with high bootstrap values (1000) at distance adjusted to 5. Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus haemolyticus and Staphylococcus saprophyticus were identified in separate clusters. However, 2 mixed clusters were resolved including Staphylococcus epidermidis and Staphylococcus warneri in one cluster, while Staphylococcus pasteuri and Staphylococcus warneri in the other one. Comparatively, the folp gene-based tree yielded 9 clusters with at distance adjusted to 5 and 1000 bootstrap value. Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus haemolyticus, Staphylococcus ludunensis, Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus saprophyticus, Staphylococcus pasteuri were resolved in separate clusters, however, Staphylococcus warneri and Staphylococcus epidermidis were identified in one mixed cluster. In addition, DNA-DNA relatedness studies indicated high sequence divergence of folp gene exhibiting 61.58-96.48 % interspecies homology compared to 16S rRNA with sequences similarities of 97.09-99.32 %. At the intraspecies level, the nucleotide substitution rates were ranged between 0-25%, mostly at low level for most Staphylococcal species compared to 98.65-99.93 identified in 16S rRNA gene. Moreover, in blind tests, this method was able for correct identification of 13 bacteria isolates of different Staphylococcal species. As a conclusion, folp gene sequences provide better resolution compared to the 16S rRNA gene sequences for both interspecies and interspecies DNA analysis of Staphylococci.

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Bioprospecting of clot buster enzyme producing Staphylococcus sp from bovine milk sample

Cited by 3 publications

References 0 publications

Detection of Sak Gene and Expression of Staphylokinase in Different Clinical Isolates of Staphyloccocus spp

Detection of Sak Gene and Expression of Staphylokinase in Different Clinical Isolates of Staphyloccocus spp

Antibacterial potential of some seaweeds species to combat biofilm-producing multi-drug resistant Staphylococcus aureus of Nile tilapia

Molecular Detection of some Staphylococcal species with the newly developed folp gene sequences

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