Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Abstract

Endophytic microorganisms live inside the plants without causing any damage to their hosts. In the agricultural field, these endophytes might be a strategy of biological control for phytopathogens. We aimed to isolate endophytic fungi from yellow passion fruit (Passiflora edulis) leaves, evaluating its biocontrol capacity by in vitro antagonism against phytopathogen Colletotrichum sp. CNPU378. We also carried out greenhouse experiments in bean seedlings. A high colonization frequency was obtained (89%), and the molecular identification based on DNA sequencing attested Colletotrichum as the most frequent genus and minor occurrence of Curvularia endophytes. The endophytes tested showed different types of competitive interactions in in vitro antagonism inhibition rate ranging from 28.8 to 48.8%. There were 10 promising antagonists tested for their antagonist activity of crude extracts of secondary metabolites, in which strain PE-36 (20.8%) stood out among the other strains evaluated. In the greenhouse assay, plants inoculated only with endophyte Colletotrichum sp. PE-36 was symptomless and suggest that the endophyte strengthened the growth promotion in common bean plants, especially in the root length and number of leaves when compared to control plants and other treatments. Despite many fungi of Colletotrichum genus being described as causative agents of anthracnose, in this study, the plant sampled was colonized predominantly by Colletotrichum endophytes living in asymptomatic relationship. By the way, we come across a Colletotrichum sp. endophyte able to antagonize a Colletotrichum sp. pathogen.

The effects of dark septate endophyte (DSE) inoculation on tomato seedlings under Zn and Cd stress

“…Sin embargo, también es importante tener en cuenta que en el caso de los hongos y en particular los endófitos pueden presentar una distribución con pocas especies abundantes y muchas especies raras (Gamboa-Gaitán, 2006;Lodge et al, 1996), similar a lo reportado por Silva (2017) en P. incarnata quien encontró endófitos pertenecientes a 16 géneros y contrario a lo reportado en maracuyá amarillo donde se encontró que la mayoría de endófitos pertenece a solo dos géneros Colletotrichum y Curvularia (Oliveira et al, 2020).…”

Plant growth-promoting activity of wild-type and bromate-resistant mutant of the endophytic fungus Colletotrichum karstii

“…Although the genus Colletotrichum has been commonly described as an agricultural important phytopathogen (Cannon et al, 2012), these fungi have also had their endophyte role successfully reported in different plant species (Bongiorno et al, 2016;Ribeiro et al, 2018;Santos et al, 2019;Golias et al, 2020;Oliveira et al, 2020).…”

“…The authors' findings corroborate results in our study for Colletotrichum mutants, especially the C. karstii SL57 mut BR-UA auxotrophic mutant, which presented an increase in solubilization activity of inorganic phosphate (23%) and the Chlb and Chlt contents in common bean plants. López-González et al (2017) and Oliveira et al (2020) have reported the Colletotrichum fungi playing an endophyte role in plants of Phaseolus sp. genus, similar to reports by Parsa et al (2016), including the C. karstii species analyzed in current study and described as a bean endophyte by the authors.…”

“…Microorganisms that asymptomatically inhabit the interior of plant tissues, called endophytes (Wenzel, Garcia, Filho, Prioli, & Pamphile, 2010;Garcia et al, 2012), may be an excellent replacement alternative for the application of chemical fertilizers without any adverse environmental effects. In fact, they are capable of solubilizing inorganic salts, produce phytohormones and antagonize phytopathogens (Polonio et al, 2015;Bongiorno et al, 2016;Ribeiro et al, 2018;Oliveira et al, 2020).…”

Técnicas De Identificação Química De Metabólitos Produzidos Por Microrganismos Endofíticos

“…Por definição, os microrganismos endofíticos, são fungos e bactérias que passam pelo menos uma parte de seu ciclo de vida colonizando inter ou intracelularmente os tecidos de plantas, não causando sintomas de doença ao seu hospedeiro (ZHAO et al 2011;BERTANI et al 2016;CHAUHAN et al 2019). Estes microrganismos têm ganhado destaque por suas aplicações biotecnológicas (ZHAO et al 2011), especialmente na agricultura (OLIVEIRA et al 2020;EMMER et al 2021;OLIVEIRA et al 2021;RIBEIRO et al 2021), medicina e indústria com a produção de metabólitos bioativos (STROBEL, 2018;GOLIAS et al 2019;POLLI et al 2020;SILVA et al 2020;SILVA et al 2021) e/ou enzimas (DA SILVA RIBEIRO et al 2018;FELBER et al 2019;SANTOS et al 2019), além de atuarem como biorremediadores de locais contaminados com substâncias recalcitrantes e xenobióticas (BULLA et al 2017).…”