Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria

Odisi, Estácio Jussie; Silvestrin, Marcela Bruschi; Takahashi, Rodrigo Yoji Uwamori; Silva, Marcus Adonai Castro da; Lima, André Oliveira de Souza

doi:10.2225/vol15-issue5-fulltext-17

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…On the other hand, Muñoz [27] isolated 17 strains with autotrophic capabilities, which implies the possibility of metabolizing inorganic molecules by synthesis or hydrolysis. Likewise, Jussie et al [28] isolated 161 strains from the South Atlantic Ocean, of which 58 had cellulolytic and/or proteolytic properties. There are reports of microbial concentrations reaching up to 3.63 × 10 4 microbial cells per milliliter in the Colombian marine muddy snows [29].…”

Section: Isolation Of Microbial Groups From Algaenzims ®mentioning

confidence: 99%

Effect of Marine Microorganisms on Limestone as an Approach for Calcareous Soil

Sánchez¹,

Jiménez²,

Escobedo‐Bocardo³

et al. 2018

Sustainability

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Calcareous soils generally have low levels of organic matter and nitrogen; they require modification to promote their support for agriculture production. Calcareous soils are commonly found in important agricultural areas throughout the world, mainly around the Mediterranean, America and Australia. In this study, we the isolated and identified different groups of microorganisms, from a product made from seaweed, in relation to their soil improvement properties. The objective was to use these microorganisms for the solubilization of specific soil elements and reduce their accumulation as a result of overfertilization. The isolated microorganisms were grown in specific culture media and were applied on limestone to determine their effect on mobility of Ca, Mg and K. Also, changes in soil properties such as pH, texture and density were evaluated. This study demonstrated that the treatments applied were able to modify the solubility of Ca, Mg and K, increasing it, in some cases, up to 3500%. In addition, an increase of organic matter close to 200% was observed. Both the group of molds and yeasts, and the group of nitrogen-fixing microorganisms, modified the proportion of sand, silt and clay in the treated limestone. These results open possibilities for the widespread use of marine microorganisms on a large scale in the agricultural sector, since they improve the nutrient availability present in the soil.

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Section: Isolation Of Microbial Groups From Algaenzims ®mentioning

confidence: 99%

Effect of Marine Microorganisms on Limestone as an Approach for Calcareous Soil

Sánchez¹,

Jiménez²,

Escobedo‐Bocardo³

et al. 2018

Sustainability

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show abstract

“…Entre los grupos fenotípicos más representados para ambos niveles de los sitios mesofóticos se destacan los bacilos G+ esporulados; donde se incluye el género Bacillus, reconocido como productores de diferentes exoenzimas (Odisi et al 2012;Lailaja & Chandrasekaran, 2013;Gawas et al 2019;Jin et al 2019).…”

Section: Discussionunclassified

“…La literatura especializada refiere que las especies de este género son productores de sustancias biológicamente activas, entre las que se encuentran antimicrobianos, tensioactivos, enzimas hidrolíticas y degradadoras de los hidrocarburos del petróleo (Sardessai & Bhosle¸ 2002;Odisi et al 2012).…”

Section: Discussionunclassified

Caracterización de bacterias cultivables aisladas de sitios de arrecifes mesofóticos de Cuba

et al. 2021

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Las comunidades bacterianas de sitios de arrecifes mesofóticos constituyen información genética de interés por las condiciones particulares de temperatura, presión, salinidad y disponibilidad de materia orgánica y nutrientes en la que se desarrollan. El presente trabajo tuvo como objetivo evaluar la diversidad bacteriana en aguas aledañas a los arrecifes mesofóticos de Cuba en mayo-junio de 2017; así como algunas de sus potencialidades biotecnológicas. Los cultivos fueron aislados en medio agar marino, en dos niveles de la columna de agua, en 10 sitios de muestreos. Posteriormente se evaluaron las capacidades metabólicas de los aislados, relacionadas con la asimilación de diferentes fuentes de carbono y la producción de metabolitos bioactivos. Los resultados permitieron la caracterización de 30 aislados bacterianos de aguas del nivel subsuperficial y 32 de aguas profundas. El mayor porcentaje corresponde a bacilos esporulados Gram positivos y bacilos Gram negativos. Asimismo, se demostró que el 85.5% degradan carbohidratos de diferente naturaleza y el 96.8% producen enzimas hidrolíticas con actividad caseinasa, gelatinasa, lipasa o amilasa. La capacidad de degradar petróleo y compuestos fenólicos indistintamente fue detectada en el 61.3% de los aislados. Entre las potencialidades de interés biomédico e industrial se detectó actividad tensioactiva y asparaginasa en el 53% y 32% de los cultivos, respectivamente. Por su parte, la caracterización taxonómica de 40 cultivos seleccionados por sus potencialidades metabólicas demostró que el género Bacillus presentó mayor actividad biológica. Estos resultados constituyen el primer acercamiento a la caracterización de la diversidad bacteriana en sitios de arrecifes mesofóticos de Cuba.

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“…occur by an enzymatic mechanism that involves synergistic actions by endoglucanase (EC 3.2.1.4), exoglucanase or cellobiohydrolase (EC 3.2.1.91), and βglucosidase (EC 3.2.1.21) [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Saccharification of Sugarcane Bagasse Using Ash - Supplemented Hydrogen Peroxide as Pre-Treatment

Odisi¹,

Lima²,

Colonetti³

et al. 2022

IJAERS

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Purpose: Enzymatic saccharification of sugarcane bagasse with cellulases was investigated after hydrogen peroxide pretreatment. Methods: Two pretreatments, alkaline hydrogen peroxide and hydrogen peroxide supplemented with ash were compared in their performance in the improvement of the susceptibility of bagasse to enzymatic action. The reaction yield was evaluated by the reducing sugar content released from the pretreated bagasse after 48 hours of enzymatic hydrolysis, and the best condition was found for both treatments. Results: The yield, expressed in reducing sugars for the alkaline hydrogen peroxide pretreatment was 217.6 mg g-1 bagasse, and 179.9 mg g-1 bagasse for hydrogen peroxide ash pretreatment; the untreated bagasse provided 74.3 mg g-1 bagasse yield, showing the effectiveness of the two pretreatments. Conclusion: The pretreatment with hydrogen peroxide supplemented with ashes appears more feasible for implementation since alkali addition in the pretreatment delivers many caustic residues that need expensive washing process and generate aggressive effluents into the environment; besides alkali addition promotes partial degradation of hemicellulose.

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Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria

Cited by 8 publications

References 26 publications

Effect of Marine Microorganisms on Limestone as an Approach for Calcareous Soil

Effect of Marine Microorganisms on Limestone as an Approach for Calcareous Soil

Caracterización de bacterias cultivables aisladas de sitios de arrecifes mesofóticos de Cuba

Enzymatic Saccharification of Sugarcane Bagasse Using Ash - Supplemented Hydrogen Peroxide as Pre-Treatment

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