Biopuces pour le traitement de cellules vivantes : micromanipulation des cellules par voie électrique ou microfluidique

Pioufle, Bruno Le; Frénéa, Marie; Tixier, A.

doi:10.1016/j.crhy.2004.02.010

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…Cell cages are used in long-term cell studies to restrict the motion of motile cells, such as neurons. [30][31][32][33][34][35] Until now, researchers have relied on various methods to place cells into such cages, but each of these methods has drawbacks. Randomly seeding cells over the vials 25 is undesirable because of poor accuracy in the position and number of cells loaded.…”

Section: Cell Loadingmentioning

confidence: 99%

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Parasitic trap cancellation using multiple frequency dielectrophoresis, demonstrated by loading cells into cages

Urdaneta

Smela

2008

Lab Chip

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This paper presents a method of using multiple frequencies to counteract electric field distortions that interfere with the dielectrophoretic (DEP) manipulation of particles or cells. To demonstrate the technique, simulations were performed for a scenario in which cells were to be loaded into a cage whose walls created parasitic trapping sites that prevented cells from entering it. By employing negative DEP on one electrode in conjunction with positive DEP on another, these traps could be almost completely cancelled. The model predictions were validated experimentally: multiple frequency DEP was used to load many cells into a cage in a matter of seconds in fluid flows of up to 300 microm s(-1), which could not be done with single frequency DEP. Actively cancelling field distortions permits the presence of features that would otherwise be prohibited near regions of dielectrophoretic manipulation, significantly expanding the environments in which dielectrophoresis can be used.

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Section: Cell Loadingmentioning

confidence: 99%

“…Manual placement 30-32, 34 is impractical for large numbers of cells. Suction loading 35 cannot readily be integrated with CMOS chips densely covered with circuits and sensors. Laser tweezers systems 33 are expensive and only allow a few cells to be loaded at one time.…”

Section: Cell Loadingmentioning

confidence: 99%

Parasitic trap cancellation using multiple frequency dielectrophoresis, demonstrated by loading cells into cages

Urdaneta

Smela

2008

Lab Chip

View full text Add to dashboard Cite

show abstract

“…The biochips as a second approach are suited for detection of molecular interactions at high degrees of parallelization [13][14][15] but are less suited for the application of cell-based tests.…”

Section: Introductionmentioning

confidence: 99%