Bioreactor-Based Tumor Tissue Engineering

Abstract

This review focuses on modeling of cancer tumors using tissue engineering technology. Tumor tissue engineering (TTE) is a new method of three-dimensional (3D) simulation of malignant neoplasms. Design and development of complex tissue engineering constructs (TECs) that include cancer cells, cell-bearing scaffolds acting as the extracellular matrix, and other components of the tumor microenvironment is at the core of this approach. Although TECs can be transplanted into laboratory animals, the specific aim of TTE is the most realistic reproduction and long-term maintenance of the simulated tumor properties in vitro for cancer biology research and for the development of new methods of diagnosis and treatment of malignant neoplasms. Successful implementation of this challenging idea depends on bioreactor technology, which will enable optimization of culture conditions and control of tumor TECs development. In this review, we analyze the most popular bioreactor types in TTE and the emerging applications.

Synthetic microparticles conjugated with VEGF165 improve the survival of endothelial progenitor cells via microRNA-17 inhibition

“…Although some biological aspects of immobilized VEGF have been explored, no study targeted the use of immobilized VEGF to improve cell survival both in vitro and in vivo 13 , 14 . Previous studies have shown that VEGF immobilized on different substrates including titanium, fibrin, and collagen is superior to sVEGF in promoting EC proliferation 36 , 37 and EC branching 22 . In addition, a previous study has shown that VEGF physically immobilized to cell culture substrates could mediate EC survival after exposure to tumstatin, a proapoptotic agent 38 .…”

Trends in Bone Metastasis Modeling

“…In dynamic bioreactor systems, the application of a fluid dynamics to the cellular microenvironment prevents the accumulation of secreted biomolecules and shear stress that can induce different forms of differentiation. Spinner-flasks and stirred-tank bioreactors are dynamic culture vessels employed to grow co-cultures of tumor cells [116], tumor cells in 3D as spheroids [117], or scaffold-based cultures [118].…”

Computational fluid dynamic analysis of bioprinted self‐supporting perfused tissue models

“…Tissue vasculature is further integrated with the cardiovascular system, which drives nutrient perfusion. Generating biofabricated tissues exhibiting similar distributed vasculature‐like structures integrated with a system capable of driving perfusion, however, has proved to be an engineering, biological, and practical challenge (Ball, Nguyen, Placone, & Fisher, ; Egger et al, ; Goldstein, Juarez, Helmke, Gustin, & Mikos, ; Stiehler et al, ; Wang, Wu, Wang, Lin, & Sun, ; Warren et al, ; Wu & Ringeisen, ), which must be overcome to generate in vivo‐like tissue structures (Carrier et al, ; Dennis, Smith, Philp, Donnelly, & Baar, ; Guller, Grebenyuk, Shekhter, Zvyagin, & Deyev, ; Maidhof et al, ).…”

“…Tissue vasculature is further integrated with the cardiovascular system, which drives nutrient perfusion. Generating biofabricated tissues exhibiting similar distributed vasculature-like structures integrated with a system capable of driving perfusion, however, has proved to be an engineering, biological, and practical challenge (Ball, Nguyen, Placone, & Fisher, 2016;Egger et al, 2017;Goldstein, Juarez, Helmke, Gustin, & Mikos, 2001;Stiehler et al, 2009;Wang, Wu, Wang, Lin, & Sun, 2009;Warren et al, 2009;Wu & Ringeisen, 2010), which must be overcome to generate in vivo-like tissue structures (Carrier et al, 2002;Dennis, Smith, Philp, Donnelly, & Baar, 2009;Guller, Grebenyuk, Shekhter, Zvyagin, & Deyev, 2016;Maidhof et al, 2012). Impeller-driven spinner flask bioreactors provide perfusion by flowing media over the tissue construct, which results in poorly controlled and poorly defined perfusion to tissues within the bulk of tissues or result in no perfusion within the bulk of solid tissues (Radisic et al, 2004;Sikavitsas, Bancroft, & Mikos, 2002).…”