Bioreactor Technology for Hairy Roots Cultivation

Mehrotra, Shakti; Mishra, Sonal; Srivastava, Vikas

doi:10.1007/978-3-319-32004-5_10-1

Cited by 8 publications

(5 citation statements)

References 90 publications

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“…Molecules 2020, 25, x FOR PEER REVIEW 3 of 22 addition, the approach also offers a relative ease in changing the scale of production, which further increases their value as potential "producers" of desired compounds [13].…”

Section: Ginsenoside Content In Studied Clones Of Hairy Root Cultures Of P Quinquefoliummentioning

confidence: 99%

“…This is especially important considering that some metabolites are synthesized only in specialized organs and usually only appear in the above-ground parts of plants. In addition, the approach also offers a relative ease in changing the scale of production, which further increases their value as potential "producers" of desired compounds [13].…”

Section: Introductionmentioning

confidence: 99%

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Panax quinquefolium L. Ginsenosides from Hairy Root Cultures and Their Clones Exert Cytotoxic, Genotoxic and Pro-Apoptotic Activity towards Human Colon Adenocarcinoma Cell Line Caco-2

et al. 2020

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American ginseng, Panax quinquefolium (L.), is traditionally used in folk medicine. It exhibits a range of anti-inflammatory, hepatoprotective, anti-diabetic, anti-obesity, anti-hyperlipidemic and anti-carcinogenic effects. Its main components are ginsenosides, also known as panaxosides or triterpene saponins. In order to obtain high yields of ginsenosides, different methods of controlled production are involved, i.e., with hairy root cultures. However, they are still employed under in vitro conditions. Our studies revealed that hairy root cultures subjected to an elicitation process can be considered as a potent source of ginsenosides. The present study examines the biological activity of ginseng hairy root cultures against the Caco-2 human adenocarcinoma cell line. Among our six different clones of P. quinquefolium hairy roots, extracts B and Be (treated with elicitor) were the strongest inhibitors of the cellular metabolic activity. While all extracts induced DNA damage, B and Be also generated reactive oxygen species (ROS) in a concentration-dependent manner, which was correlated with the depletion of the mitochondrial membrane potential and induction of apoptosis. These findings indicate that further research concerning P. quinquefolium hairy root cultures should focus on the activity of rare ginsenosides and other biologically active compound profiles (i.e., phenolic compounds).

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Section: Ginsenoside Content In Studied Clones Of Hairy Root Cultures Of P Quinquefoliummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Panax quinquefolium L. Ginsenosides from Hairy Root Cultures and Their Clones Exert Cytotoxic, Genotoxic and Pro-Apoptotic Activity towards Human Colon Adenocarcinoma Cell Line Caco-2

et al. 2020

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“…Many published review articles and a wide range of scientific reports notify that the solution for most of the obstacles related to plant biomass harvesting for secondary metabolites production comes with in vitro bioprocessing of hairy roots [ 6 , 7 , 8 , 9 ]. Due to stable genetic alterations induced by Rhizobium rhizogenes , hairy root features intensified growth in a culture media free of plant growth regulators, with the propensity for lateral branching and enhanced biosynthesis of secondary metabolites specific for root tissue differentiation [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Selective Impact of MTMS-Based Xerogel Morphology on Boosted Proliferation and Enhanced Naphthoquinone Production in Cultures of Rindera graeca Transgenic Roots

Wierzchowski

Nowak

Kawka

et al. 2022

IJMS

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In situ extraction is a method for separating plant secondary metabolites from in vitro systems of plant biomass cultures. The study aimed to investigate the MTMS-based xerogels morphology effect on the growth kinetics and deoxyshikonin productivity in xerogel-supported in vitro culture systems of Rindera graeca hairy root. Cultures were supplemented with three types of xerogel, i.e., mesoporous gel, microporous gel, and agglomerated precipitate, in the disintegrated or monolithic form. Structure, oil sorption capacity, and SEM analyses for xerogel-based additives were performed. Application of monolithic macroporous xerogel resulted in the highest biomass proliferation, i.e., 5.11-fold fresh biomass increase after four weeks of the screening culture. The highest deoxyshikonin production (i.e., 105.03 µg) was noted when hairy roots were maintained with particles of disintegrated mesoporous xerogel. The detailed kinetics investigations (6-week culture) revealed the highest growth of hairy root biomass and secondary metabolite production, equaling 9.46-fold fresh weight biomass and 204.08 µg deoxyshikonin, respectively. MTMS-based xerogels have been recognized as selective biocompatible scaffolds for boosting the proliferation of transgenic roots or for productivity enhancement of naphthoquinones without detrimental effects on biomass growth, and their successful applicability in in situ removal of secondary plant metabolites has been experimentally confirmed.

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“…Many different bioreactors have been created so far, with new designs and improvements still coming out. However, the basic bioreactor types, including those with liquid and gas phase reactors or hybrid reactors, are still frequently used in many studies [21][22][23][24][25]. Apart from hairy roots, micropropagated plants and cell cultures were also used as sources for the production of secondary metabolites in bioreactors.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, since the same explant types displayed different growth potential and the ability to produce secondary metabolites [32][33][34], the specific culture conditions are also necessary to be determined in the proposed bioreactors. Accordingly, some bioreactors were designed for specific purposes [35] and a number of them were adapted for some specific plant species and exploitation purposes [21][22][23][24][25]36].…”

Section: Introductionmentioning

confidence: 99%

Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Vinterhalter

Banjac

Vinterhalter

et al. 2021

Plants

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The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

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Bioreactor Technology for Hairy Roots Cultivation

Cited by 8 publications

References 90 publications

Panax quinquefolium L. Ginsenosides from Hairy Root Cultures and Their Clones Exert Cytotoxic, Genotoxic and Pro-Apoptotic Activity towards Human Colon Adenocarcinoma Cell Line Caco-2

Panax quinquefolium L. Ginsenosides from Hairy Root Cultures and Their Clones Exert Cytotoxic, Genotoxic and Pro-Apoptotic Activity towards Human Colon Adenocarcinoma Cell Line Caco-2

Selective Impact of MTMS-Based Xerogel Morphology on Boosted Proliferation and Enhanced Naphthoquinone Production in Cultures of Rindera graeca Transgenic Roots

Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

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