Bioreactors for Cardiac Tissue Engineering

Paez‐Mayorga, Jesus; Hernández-Vargas, Gustavo; Ruiz–Esparza, Guillermo U.; Iqbal, Hafiz M.N.; Wang, Xichi; Zhang, Yu Shrike; Parra‐Saldívar, Roberto; Khademhosseini, Ali

doi:10.1002/adhm.201701504

Cited by 56 publications

(27 citation statements)

References 78 publications

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“…However, as mentioned previously, the canonical 2D culture and differentiation of these cells still has major limitations, most notably their immature phenotype, which lacks to fully represent developed tissue. The development of new biomaterials (Reis et al, 2016;Wissing et al, 2017;Kuraitis et al, 2019) and the emerging of new technologies such as tissue printing (Charbe et al, 2019;Tomov et al, 2019), organ on a chip (Marsano et al, 2016;Ugolini et al, 2018;Wan et al, 2018), and different types of bioreactors that allow mechanical, perfusion, or electrical stimulation (Freed et al, 2006;Lei and Ferdous, 2016;Paez-Mayorga et al, 2019) allow us to generate cardiac tissue that more closely recapitulate the (patho)physiological features of the developed myocardium. Moreover, these models represent an optimal tool not only to test and validate new drugs or to re-create tissue substitute for regenerative medicine application but also to allow a better understanding of the molecular mechanisms behind disease development and progression.…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

When Stiffness Matters: Mechanosensing in Heart Development and Disease

Gaetani

Zizzi

Deriu

et al. 2020

Front. Cell Dev. Biol.

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During embryonic morphogenesis, the heart undergoes a complex series of cellular phenotypic maturations (e.g., transition of myocytes from proliferative to quiescent or maturation of the contractile apparatus), and this involves stiffening of the extracellular matrix (ECM) acting in concert with morphogenetic signals. The maladaptive remodeling of the myocardium, one of the processes involved in determination of heart failure, also involves mechanical cues, with a progressive stiffening of the tissue that produces cellular mechanical damage, inflammation, and ultimately myocardial fibrosis. The assessment of the biomechanical dependence of the molecular machinery (in myocardial and non-myocardial cells) is therefore essential to contextualize the maturation of the cardiac tissue at early stages and understand its pathologic evolution in aging. Because systems to perform multiscale modeling of cellular and tissue mechanics have been developed, it appears particularly novel to design integrated mechano-molecular models of heart development and disease to be tested in ex vivo reconstituted cells/tissue-mimicking conditions. In the present contribution, we will discuss the latest implication of mechanosensing in heart development and pathology, describe the most recent models of cell/tissue mechanics, and delineate novel strategies to target the consequences of heart failure with personalized approaches based on tissue engineering and induced pluripotent stem cell (iPSC) technologies.

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Section: Conclusion and Future Perspectivementioning

confidence: 99%

When Stiffness Matters: Mechanosensing in Heart Development and Disease

Gaetani

Zizzi

Deriu

et al. 2020

Front. Cell Dev. Biol.

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“…The definition of tissue engineering has been very elegantly presented in a recent publication: 10,11 "Tissue engineering is a multidisciplinary field bringing together experts from engineering, life sciences and medicine, utilizing the building blocks of cells, biomaterials and bioreactors for the development of 3-dimensional artificial tissue and organs which can be used to augment, repair and/or replace damaged and/or diseased tissue." This definition truly embodies the key elements of the field and is divided into three main components.…”

Section: Definition Of Tissue Engineeringmentioning

confidence: 99%

3D bioprinting and its potential impact on cardiac failure treatment: An industry perspective

Birla

Williams

2020

APL Bioengineering

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3D printing technologies are emerging as a disruptive innovation for the treatment of patients in cardiac failure. The ability to create custom devices, at the point of care, will affect both the diagnosis and treatment of cardiac diseases. The introduction of bioinks containing cells and biomaterials and the development of new computer assisted design and computer assisted manufacturing systems have ushered in a new technology known as 3D bioprinting. Small scale 3D bioprinting has successfully created cardiac tissue microphysiological systems. 3D bioprinting provides an opportunity to evaluate the assembly of specific parts of the heart and most notably heart valves. With the continuous development of instrumentation and bioinks and a complete understanding of cardiac tissue development, it is proposed that 3D bioprinting may permit the assembly of a heart described as a total biofabricated heart.

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“…Васкуляризация растущей сердечной структуры базируется на добавлении в инкубирующую среду факторов сосудистого роста, и в отдельных случаях получали успешное сокультурирование кардиомиоцитов и сосудистых клеток [42][43][44][45]. Несмотря на некоторые успехи в выращивании кровеносного русла и, соответственно, относительно успешную культивацию 2-3 мм слоев сердечных клеток, для трансплантации подобные мышечные лоскуты оказываются малопригодны, так как сосудистое русло имплантата развивается спонтанно и без всякого соответствия с сосудистым руслом реципиента [46][47][48].…”

Section: культивирование элементов клеточной стенкиunclassified

Cell technologies in the regenerative medicine of the heart: main problems and ways of development

Agladze

2019

Alʹm. klin. med.

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The potential of heart tissues for self-regeneration is not high and supposedly limited to a small number of the niche stem cells. This makes it extremely important to develop regenerative technologies for the myocardium based on modern techniques, for instance, cell re-programming and 3D bioprinting. However, it is often difficult to differentiate the sensational reports regularly appearing in mass media on “breakthrough” technologies from those that really have practical applications. The article sets out a point of view on the popular technologies for the regeneration of cardiac tissues and myocardium as a whole and reviews their drawbacks. The main problems of the bioprinting approach being actively developed include a low differentiation level with printing by stem cells that does not allow for a full-fledged cardiac tissue without foreign inclusions, as well as technological impossibility, when printing with stem cells, to set up their links with other cells during cell delivery in their corresponding matrix locations. Despite some optimistic reports on the good performance on stem or induced pluripotent cells injections into the myocardial injury zone that were first made public about 20 years ago, nowadays this idea seems rather doubtful, because in the recent years there has been virtually no positive effect of this procedure with a serious risk of complications. As far as growing of heart muscle elements is concerned, the main challenge is the development of the “proper” vascularization of the muscle being grown. At the same time, one has to emphasize practical feasibility of growing relatively small myocardial elements, such as sinus node.

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Bioreactors for Cardiac Tissue Engineering

Cited by 56 publications

References 78 publications

When Stiffness Matters: Mechanosensing in Heart Development and Disease

When Stiffness Matters: Mechanosensing in Heart Development and Disease

3D bioprinting and its potential impact on cardiac failure treatment: An industry perspective

Cell technologies in the regenerative medicine of the heart: main problems and ways of development

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