Biorecognition and detection of antigens from Mycobacterium tuberculosis using a sandwich ELISA associated with magnetic nanoparticles

Self-assembly of amino-terminated monolayers depending on the chemical structure

“…4,5 Amino-terminated SAMs prepared from commercially available silanes such as 3-aminopropyl-triethoxysilane (APTES) and 3-aminopropyl-trimethoxysilane (APTMS) are mostly used to promote the biofunctionalization of a solid support in bioanalytical platforms, 6 to study protein or cell adhesion, 7 and to functionalize (nano)particles. [8][9][10] The formation of an aminosilane layer is sensitive to the reaction conditions, and the quality of the APTES or APTMS films depends on different parameters such as concentration, 11 the nature of the solvent, 12 time, 13 temperature, 14 humidity level, 15 and post-deposition treatment (the annealing step). 16,17 Many experimental conditions were described to deposit aminosilane films by wet chemistry, giving rise to monolayer 18,19 or multilayer 20 films.…”

Evolution of tuberculosis diagnostics: From molecular strategies to nanodiagnostics

“…The functionalized MNP@Si@ab was tested in a colorimetric sandwich enzyme-linked immunosorbent assay (sELISA-MNP@Si@ab) to identify specific antigens in sputum samples. This method is rapid as compared to the conventional ELISA and also exhibits higher sensitivity in the clinical samples [ 183 ]. Engstrom and his co-workers developed streptavidin-tagged magnetic nanobead labelled with biotin to detect mutation in the rpoB gene of Mtb responsible for antibiotic resistance.…”