Bioreduction of hexavalent chromium by Pseudomonas stutzeri L1 and Acinetobacter baumannii L2

Sathishkumar, Kuppusamy; Murugan, Kadarkarai; Benelli, Giovanni; Higuchi, Akon; Rajasekar, Aruliah

doi:10.1007/s13213-016-1240-4

Cited by 63 publications

(12 citation statements)

References 35 publications

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“…M6 had a 45.9% removal at 200 mg/L Cr(VI) concentration, but the Cr(VI) concentration in the control did not change signi cantly(Li et al 2019). It has been reported that Pseudomonas stutzeri L1 and Acinetobacter baumannii L2 strains reduce Cr(VI) by 40-97% and 84-99%, respectively, at concentrations of 100-1000 mg/L(Sathishkumar et al 2017). When we look at the studies on Cr(VI) removal with recombinant strains; as a result of the cloning of the ChrA, ChrB and ChrAB genes associated with Cr(VI) resistance of Serratia sp.…”

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confidence: 86%

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Akkurt¹,

Oğuz²,

Uçkun³

2021

Preprint

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Studies on the removal of toxic metals from water by genetic engineering applications are becoming more and more diverse. Especially by the expression of cysteine and thiol rich proteins such as metallothioneins in bacterial cells, a high rate of removal of metal ions from the environment is ensured. In this study, we evaluated the removal and reduction of hexavalent chromium Cr(VI)from aqueous solutions with the recombinant strains obtained by cloning the human metallothioneinsMT2A and MT3 into Escherichia coli Jm109.E. coli MT2A was the most effective strain in both Cr(VI) removal (89%in 25 mg/L Cr(VI) application) and Cr(VI) reduction (76%in 25 mg/L Cr(VI) application).Cr(VI) adsorbed per dry cell (at 25 mg/L) were 17 mg/g, 22 mg/g and 19 mg/g for Jm109, MT2A and MT3, respectively. Scanning electron microscope (SEM) images showed that the morphological structures of Cr(VI)treated cells were significantly damaged compared to control cells. Scanning transmission electron microscope (STEM) images showed black spots in the cytoplasm of cells treated with Cr(VI).From the shifts in the Fourier transform infrared spectroscopy analysis (FTIR) spectra of the cells treated with Cr(VI) compared to the control cells, it was determined that the groups interacting with Cr were amino, hydroxyl, methyl and sulfhydryl. When all these experimental data were evaluated together, it was concluded that all three species were effective in removing Cr(VI) from aqueous solutions and MT2A strain could be used as the most effective biotechnological tool.

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confidence: 86%

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Akkurt¹,

Oğuz²,

Uçkun³

2021

Preprint

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confidence: 99%

Bioreduction and bioremoval of hexavalent chromium by genetically engineered strains (Escherichia coli MT2A and Escherichia coli MT3)

Akkurt

Oğuz

Uçkun

2022

World J Microbiol Biotechnol

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Studies on the removal of toxic metals from water by genetic engineering applications are becoming more and more diverse. Especially by the expression of cysteine and thiol rich proteins such as metallothioneins in bacterial cells, a high rate of removal of metal ions from the environment is ensured. In this study, we evaluated the removal and reduction of hexavalent chromium Cr(VI)from aqueous solutions with the recombinant strains obtained by cloning the human metallothioneinsMT2A and MT3 into Escherichia coli Jm109.E. coli MT2A was the most effective strain in both Cr(VI) removal (89%in 25 mg/L Cr(VI) application) and Cr(VI) reduction (76%in 25 mg/L Cr(VI) application).Cr(VI) adsorbed per dry cell (at 25 mg/L) were 17 mg/g, 22 mg/g and 19 mg/g for Jm109, MT2A and MT3, respectively. Scanning electron microscope (SEM) images showed that the morphological structures of Cr(VI)treated cells were signi cantly damaged compared to control cells. Scanning transmission electron microscope (STEM) images showed black spots in the cytoplasm of cells treated with Cr(VI).From the shifts in the Fourier transform infrared spectroscopy analysis (FTIR) spectra of the cells treated with Cr(VI) compared to the control cells, it was determined that the groups interacting with Cr were amino, hydroxyl, methyl and sulfhydryl. When all these experimental data were evaluated together, it was concluded that all three species were effective in removing Cr(VI) from aqueous solutions and MT2A strain could be used as the most effective biotechnological tool.

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“…Therefore, during cleanup of soil from toxic Cr(VI) compounds, it is optimal to convert it into insoluble and non-toxic hydroxide directly in soils, because moisture and microbial exometabolites are unable to mobilize it. For example, Pseudomonas stutzeri was able to reduce Cr(VI) compounds (Sathishkumar et al, 2017). Bacillus brevis was resistant to elevated Cr(VI) levels and may potentially reduce it in short time from in the environment where other metal ions are also present in addition to chromium ions (Verma et al, 2009).…”

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confidence: 99%

Bioremoval of hazardous cobalt, nickel, chromium, copper and cadmium compounds from contaminated soil by Nicotiana tabacum plants and associated microbiome

et al. 2021

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Contamination of soils with heavy metals leads to reduction of soil fertility, destruction of natural ecosystems and detrimental effects on the health of society by increasing content of metals in the food chains from microorganisms to plants, animals and humans. Bioremediation is one of the most promising and cost-effective methods of cleaning soils polluted with toxic metals. According to current researchers, microorganisms and plants have the genetic potential to remove toxic metals from contaminated sites. The method of thermodynamic prediction was used to theoretically substantiate the mechanisms of interaction of soil microorganisms and plants with heavy metals. According to the our prediction, exometabolite chelators of anaerobic microorganisms may increase the mobility of metals and thereby contribute to the active transport of metals and their accumulation in plants. Plants of Nicotiana tabacum L. of Djubek cultivar were used as plant material for the current investigation. The examined toxicants were heavy metals, namely cobalt (II), nickel (II), chromium (VI), copper (II) and cadmium (II). The aqueous solutions of metal salts were added to the boxes after two months of plants growing to the final super-high concentration – 500 mg/kg of absolutely dry weight of soil. Quantitative assessments of copper and chromium-resistant microorganisms were made by cultivation on agar nutrient medium NA with a gradient of Cu(II) and Cr(VI). The concentration of metals in soil and plant material (leaves, stems and roots) was determined by atomic absorption method. The study revealed that heavy metals inhibited the growth of the examined tobacco plants. This was expressed by the necrosis of plant tissues and, ultimately, their complete death. Despite this, all investigated heavy metals were accumulated in plant tissues during 3–7 days before death of plants. The uptake of metals was observed in all parts of plants – leaves, stems and roots. The highest concentrations of Co(II), Ni(II), Cd(II), Cr(VI) were found in the leaves, Cu(II) – in the roots. The results show that the bioremoval efficiency of the investigated metals ranged 0.60–3.65%. Given the super-high initial concentration of each of the metals (500 mg/kg), the determined removal efficiency was also high. Cadmium was the most toxic to plants. Thus, the basic points of the thermodynamic prognosis of the possibility of accumulation of heavy metals by phytomicrobial consortium were experimentally confirmed on the example of N. tabacum plants and metal-resistant microorganisms. The study demonstrated that despite the high initial metals concentration, rate of damage and death of plants, metals are accumulated inplant tissues in extremely hight concentrations. Soil microorganisms were observed to have high adaptation potencial to Cu(II) and Cr(VI). In anaerobic conditions, microorganisms presumably mobilize heavy metals, which later are absorbed by plants. The obtained results are the basis for the development of environmental biotechnologies for cleaning contaminated soils from heavy metal compounds.

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Bioreduction of hexavalent chromium by Pseudomonas stutzeri L1 and Acinetobacter baumannii L2

Cited by 63 publications

References 35 publications

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Bioreduction and bioremoval of hexavalent chromium by genetically engineered strains (Escherichia coli MT2A and Escherichia coli MT3)

Bioremoval of hazardous cobalt, nickel, chromium, copper and cadmium compounds from contaminated soil by Nicotiana tabacum plants and associated microbiome

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