Biorefining via solid-state fermentation of rice and sunflower by-products employing novel monosporic strains from Pleurotus sapidus

Biotransformation of rice and sunflower side-streams by dikaryotic and monokaryotic strains of Pleurotus sapidus: Impact on phenolic profiles and bioactive properties

“…The dikaryotic strain Pleurotus sapidus Dk3174 (Institut für Lebensmittelchemie (LCI) fungi collection No. 343, Leibniz Universität Hannover, Germany) obtained by crossing two compatible monokaryons, Mk31 and Mk74 (Omarini et al, 2014) and the monokaryon P6 (Mk) derived from a germinated spore of the Dk (Dk3174) were employed (Postemsky et al, 2019). Strains were cultured in potato/ dextrose/agar medium (PDA, Carl Roth, Karlsruhe, Germany) for seven days at 25°C and stored at 4°C.…”

“…2.4. Solid-state fermentation of rice and sunflower side-streams SSF of rice straw (RS), rice husks (RH) and sunflower seed hulls (SH) by the monokaryon P6 (MkP6) and the dikaryon Dk3174 (Dk) at pilotscale conditions was performed as previously described (Postemsky et al, 2019). Dried RS (10-14% of humidity) was chopped until a particle size of 5-10 cm, while RH and SH (9-12% of humidity), were used directly.…”

“…The lignocellulosic material represents a valuable resource of bioactive compounds such as polysaccharides, hydrocarbons, polyphenols, organic acids, vitamins, fatty acids, proteins among others. Furthermore, it can be used as raw material to produce edible mushrooms, enzymes, nanocomposites or protein rich material (Bilal & Iqbal, 2019;Omarini et al, 2019;Postemsky et al, 2019;Sadh et al, 2018;Torres-León et al, 2018). Additionally, antinutritional factors present in the plant material can be minimized through biotechnological processes for use as a food additive or in the formulation of balanced food for human or as animal fodder (Ghorai et al, 2009;Torres-León et al, 2018).…”

“…In particular, P. sapidus is easy to cultivate in solid and liquid fermentation, it has a short life cycle and it has demonstrated its potential for metabolite production and compound bioconversion. Additionally, it was determined that this fungus can generates monokaryotic strains which offer an interesting genotypic and phenotypic traits diversity and this fact opens new possibilities for their application as non-GMO biocatalysts in different processes (Omarini et al, 2019;Omarini, Plagemann, Schimanski, Krings, & Berger, 2014;Postemsky et al, 2019).…”

Effects of mixed agro-residues (corn crop waste) on lignin-degrading enzyme activities, growth, and quality ofLentinula edodes

“…1 Macrofungi are very important species that use mycelia to undergo solid-state fermentation (SSF) and produce characteristic fruiting bodies. 2 Indeed, macrofungi grow on every type of organic matter and recycle substrate nutrients. Lentinula edodes, commonly known as shiitake mushrooms, are a highly sought aer, edible, and valuable mushroom that are widely cultivated in China (10.43 million tons per year), Japan, and South Korea.…”