Bioremediation and biomass production from the cultivation of probiotic Saccharomyces boulardii in parboiled rice effluent

Gaboardi, Giana; Santos, Diego Gil de los; Mendes, Lantier; Centeno, Larissa; Meireles, Taiane Pontes; Vargas, Samantha; Griep, Emili; Silva, Arthur de Castro Jorge; Moreira, Ângela Nunes; Conceição, Fabrício Rochedo

doi:10.1016/j.jenvman.2018.08.027

Cited by 7 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same author had already demonstrated the potential of P. pastoris X-33 as a bioremediator microorganism, finding that the yeast grown in parboiled rice effluent supplemented with 15 g.L −1 of biodiesel glycerol promoted reductions in COD - chemical oxygen demand (55%), phosphorus (52%), and nitrogen (45%) 23 . Recently, the cultivation of the probiotic Saccharomyces boulardii in this effluent reduced COD, nitrogen and phosphorus concentrations, reinforcing the possibility of producing probiotic yeasts in effluent and simultaneously reducing the environmental parameters 65 . These combined results bring a new approach to P. pastoris , which has been used for decades as a heterologous system for protein expression 16 .…”

Section: Discussionmentioning

confidence: 99%

Influence of Pichia pastoris X-33 produced in industrial residues on productive performance, egg quality, immunity, and intestinal morphometry in quails

Gaboardi

Alves

Santos

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

This study was conducted in quails to evaluate the probiotic potential of Pichia pastoris X-33, cultivated in parboiled rice effluent supplemented with biodiesel glycerol or in standard medium Yeast Extract–Peptone–Dextrose (YPD). Forty-days-old female quails were divided into three treatments: T1 (Control) received a basal diet without P. pastoris; T2 (Pichia Effluent) received a basal diet supplemented with P. pastoris grown in parboiled rice effluent and biodiesel glycerol, and T3 (Pichia YPD) received a basal diet supplemented with P. pastoris produced in YPD. The birds were vaccinated against Newcastle Disease (NDV), Avian Infectious Bronchitis (IBV), and Gumboro Disease on days 1 and 28. The following parameters were analyzed: performance, egg quality, humoral immune response to the vaccines, organ weight, and intestinal morphometry. P. pastoris grown in YPD increased egg weight (p < 0.05). The lowest liver weight on day 14 was obtained in Pichia Effluent, whereas both P. pastoris supplemented groups had the lowest duodenum weights on day 14. Besides that, livers and duodenums presented no morphological changes in any of the three treatments. Supplementation of P. pastoris modulated the immune system of the birds, increasing anti-IBV, anti-NDV, and anti-Gumboro antibodies levels compared to the Control (p < 0.05). In conclusion, quail’s immune response was improved by Pichia pastoris X-33, either it was grown in YPD or industrial residues, and the egg weight increased with Pichia pastoris X-33 grown in YPD, thereby demonstrating to be a promising probiotic for poultry.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of Pichia pastoris X-33 produced in industrial residues on productive performance, egg quality, immunity, and intestinal morphometry in quails

Gaboardi

Alves

Santos

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Xu et al (2019) compared the use of wheat gluten and soybean protein enzymatic hydrolysates for the growth of Saccharomyces pastorianus, achieving cell growth of 9.23 and 9.85 g/L, respectively. Gaboardi et al (2018) studied the use of parboiled rice effluent from maceration tanks for the growth of probiotic Saccharomyces boulardii, producing 3.8 g/L of biomass.…”

Section: Single-cell Proteinmentioning

confidence: 99%

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

et al. 2020

View full text Add to dashboard Cite

The integrated approach in biorefinery mainly involves the utilization of various agroindustrial byproducts such as raw materials for the production of several biobased products like biofuels, bioenergy, and other high-value chemicals. Biofuels are the backbone of biorefineries, however, production of value-added biomolecules such as biopigments, biopolymers, biosurfactants, and nutritional yeast has been attracting great attention. The production of these biomolecules using traditional approaches has been extensively studied in the last few years owing to their promising application in different industries such as chemical, food/feed, and pharmaceuticals for the development of novel products for mankind. Moreover, the production of such biomolecules using lignocellulosic, starchy, and some other agroindustrial byproducts is still not fully explored. Hence, there is a huge scope in the development of sustainable biorefining approaches to make the technology cost-effective. The lignocellulosic biomasses usually used in biorefineries are mainly composed of cellulose, hemicellulose, and lignin, whereas starchy materials, besides starch, usually contain, protein, lipids, and some micronutrients. The processing of these biomasses through successive steps like pretreatments, enzymatic hydrolysis, and fermentation is essentially required to obtained final biobased products. Considering certain bottlenecks of above-mentioned conventional biorefineries approaches, new technologies have been proposed for the improved pretreatment of biomass and efficient enzymatic hydrolysis in order to minimize the concentration of toxic inhibitors in resulting hydrolysate. In this review, we highlighted the different agroindustrial byproducts and their applications for the production of valuable biorefinery products.

show abstract

Engineering Saccharomyces boulardii for Probiotic Supplementation of l‐Ergothioneine

Tang,

Zhang,

Wang

et al. 2024

Biotechnology Journal

View full text Add to dashboard Cite

Saccharomyces boulardii, as a probiotic yeast, has shown great potential in regulating gut health and treating gastrointestinal diseases. Due to its unique antimicrobial and immune‐regulating functions, it has become a significant subject of research in the field of probiotics. In this study, we aim to enhance the antioxidant properties of S. boulardii by producing l‐ergothioneine (EGT). We first constructed a double knockout of ura3 and trp1 gene in S. boulardii to facilitate plasmid‐based expressions. To further enable effective genome editing of S. boulardii, we implemented the PiggyBac system to transpose the heterologous gene expression cassettes into the chromosomes of S. boulardii. By using enhanced green fluorescent protein (EGFP) as the reporter gene, we achieved random chromosomal integration of EGFP expression cassette. By using PiggyBac transposon system, a great variety of EGT‐producing strains was obtained, which is not possible for the conventional single target genome editing, and one best isolated top producer reached 17.50 mg/L EGT after 120 h cultivation. In summary, we have applied the PiggyBac transposon system to S. boulardii for the first time for genetic engineering. The engineered probiotic yeast S. boulardii has been endowed with new antioxidant properties and produces EGT. It has potential applications in developing novel therapeutics and dietary supplements for the prevention and treatment of gastrointestinal disorders.

show abstract

Bioremediation and biomass production from the cultivation of probiotic Saccharomyces boulardii in parboiled rice effluent

Cited by 7 publications

References 22 publications

Influence of Pichia pastoris X-33 produced in industrial residues on productive performance, egg quality, immunity, and intestinal morphometry in quails

Influence of Pichia pastoris X-33 produced in industrial residues on productive performance, egg quality, immunity, and intestinal morphometry in quails

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

Engineering Saccharomyces boulardii for Probiotic Supplementation of l‐Ergothioneine

Contact Info

Product

Resources

About