Bioremediation of 60Co from simulated spent decontamination solutions

Rashmi, K.; Sowjanya, T. Naga; Mohan, P. Maruthi; Balaji, V.; Venkateswaran, G.

doi:10.1016/j.scitotenv.2004.02.009

Cited by 16 publications

(8 citation statements)

References 15 publications

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“…The presently observed removal capacities are much higher than that observed in our previous studies using bioengineered E. coli with NiCoT genes of RP and NA (Raghu et al 2008;Duprey et al 2014) and mutant variety of simple bacteria and fungi (Rashmi et al 2007(Rashmi et al , 2004. In conclusion, this study reports for the first time the efficient *Co removal by recombinant DR-RP and DR-NA (12.0 μg of cobalt) when compared to fungi Neurospora crassa, N. crassa CSM-9, and E. coli DH5α (0.02, 0.04, and 1.0 μg, respectively).…”

Section: Discussioncontrasting

confidence: 65%

“…5b). The studies on trace Co removal from simulated effluents containing a large excess (>10 5 ) of heterogeneous other metal ions (Fe, Cr, and Ni) using bioengineered radiation-resistant bacteria reported in this work are indeed very significant advancements over their previous efforts using several species of bacteria, fungi (Rashmi et al 2007(Rashmi et al , 2004, and bioengineered E. coli (ARY023) with NiCoT gene of R. palustris and N. aromaticivorans (Raghu et al 2008). The problem is that bioengineered E. coli hardly survives up to 20 Gy during the Co removal, because of γ-radiation toxicity, and the survival values for recombinant E. coli and DR at 20 Gy are≈1.125×10 7 and≈2.95×10 7 CFU ml −1 , respectively (Raghu et al 2008).…”

Section: *Co Removal Efficiency By Transformed E Coli and Deinococcumentioning

confidence: 71%

“…These studies demonstrated significant *Co removal ranging from 20 to 40 ng/g biomass by fungi and 1 μg/g biomass bacteria from simulated spent decontamination effluents containing 34 nM (2 ppb) of total cobalt (Rashmi et al 2004(Rashmi et al , 2007. Genetically bioengineered E. coli, with Ni/Co transporter (NiCoT) genes from Rhodopseudomonas palustris CGA009 (RP) and Novosphingobium aromaticivorans F-199 (NA), were successfully used for removal of trace cobalt (*Co) from aqueous solutions (Raghu et al 2008;Duprey et al 2014).…”

Section: Introductionmentioning

confidence: 87%

See 2 more Smart Citations

Engineered Deinococcus radiodurans R1 with NiCoT genes for bioremoval of trace cobalt from spent decontamination solutions of nuclear power reactors

Gogada

Singh

Lunavat

et al. 2015

Appl Microbiol Biotechnol

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The aim of the present work was to engineer bacteria for the removal of Co in contaminated effluents. Radioactive cobalt ((60)Co) is known as a major contributor for person-sievert budgetary because of its long half-life and high γ-energy values. Some bacterial Ni/Co transporter (NiCoT) genes were described to have preferential uptake for cobalt. In this study, the NiCoT genes nxiA and nvoA from Rhodopseudomonas palustris CGA009 (RP) and Novosphingobium aromaticivorans F-199 (NA), respectively, were cloned under the control of the groESL promoter. These genes were expressed in Deinococcus radiodurans in reason of its high resistance to radiation as compared to other bacterial strains. Using qualitative real time-PCR, we showed that the expression of NiCoT-RP and NiCoT-NA is induced by cobalt and nickel. The functional expression of these genes in bioengineered D. radiodurans R1 strains resulted in >60 % removal of (60)Co (≥5.1 nM) within 90 min from simulated spent decontamination solution containing 8.5 nM of Co, even in the presence of >10 mM of Fe, Cr, and Ni. D. radiodurans R1 (DR-RP and DR-NA) showed superior survival to recombinant E. coli (ARY023) expressing NiCoT-RP and NA and efficiency in Co remediation up to 6.4 kGy. Thus, the present study reports a remarkable reduction in biomass requirements (2 kg) compared to previous studies using wild-type bacteria (50 kg) or ion-exchanger resins (8000 kg) for treatment of ~10(5)-l spent decontamination solutions (SDS).

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Section: Discussioncontrasting

confidence: 65%

Section: *Co Removal Efficiency By Transformed E Coli and Deinococcumentioning

confidence: 71%

Section: Introductionmentioning

confidence: 87%

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Engineered Deinococcus radiodurans R1 with NiCoT genes for bioremoval of trace cobalt from spent decontamination solutions of nuclear power reactors

Gogada

Singh

Lunavat

et al. 2015

Appl Microbiol Biotechnol

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“…However, Co 2+ removal efficiencies varied if other cations were also present ( Figure 4) [28]. Rashmi [29] reported that a mutant fungus grown in iron-bearing medium before exposure to a simulated decontamination solution containing 0.033 mmol/L Co 2+ -EDTA exhibited increased or only marginally reduced(<10%) cobalt bioaccumulation capacity despite the presence of a high concentration of iron in the growth medium (initial[Fe/Co] = 2.5 £ 10 3 ). The present data suggest that R. palustris may have evolved a specific mechanism to tolerate and accumulate high levels of cobalt in hostile environments.…”

Section: Effect Of Co-cations On Cobalt Bioaccumulationmentioning

confidence: 99%

Cobalt(II) bioaccumulation and distribution in Rhodopseudomonas palustris

Gao

Wang

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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Bioaccumulation by growing cells is a potential technique for the removal of heavy metals from wastewater. Cobalt bioaccumulation by Rhodopseudomonas palustris via different metabolic pathways was investigated for various pH levels, temperatures, co-cations and initial Co 2+ concentrations. The distribution of cobalt and its chemical nature in R. palustris were examined by cell compartmentalization and X-ray diffraction analysis. Our results indicated that biomass and bioaccumulation capacity were superior under aerobic conditions in the dark at pH 6.5 and 30 C. Cobalt removal efficiency and bioaccumulation capacity were, respectively, 84. 9% and 287.18 . Cobalt was distributed between the cell surface, periplasmic space, membrane and cytoplasm. The periplasmic space and membrane accounted for 57.37% and 27.95% of Co 2+ uptake, respectively. X-ray diffraction analysis showed that cobalt ions were predominantly deposited in the cell as cobalt phosphate octahydrate. These results demonstrate that R. palustris could be used to remove cobalt from industrial wastewater; it would therefore be beneficial to further study the mechanism of cobalt bioaccumulation in this organism.

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“…A number of studies are now available which focus on the bioremediation of toxic metals, which either remove the metals from the waste by bioadsorption [4][5][6] or convert the chemical properties of the metals by reduction [7,8]. These methods, however, may not be sufficient for treating wastes with organometallic compounds where biodegradation of organic matter is essential.…”

mentioning

confidence: 99%

Bioremediation of Organometallic Compounds by Bacterial Degradation

Shah

Dahanukar

2011

Indian J Microbiol

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The use of organometallic compounds in the environment is constantly increasing with increased technology and progress in scientific research. But since these compounds are fairly stable, as metallic bonds are stable, they are difficult to degrade or decompose naturally. The aim of this work was to isolate and characterize heterotrophic bacteria that can degrade organometallic compounds (in this case 'ferrocene' and its derivatives). A Gram-negative coccobacillus was isolated from a rusting iron pipe draining into a freshwater lake, which could utilize ferrocene as a sole source of carbon. Phylogenetic analysis based on 16S rDNA sequence suggested that the isolated organism resembled an environmental isolate of Bordetella. Ferrocene degradation was confirmed by plotting the growth curve of the bacterium in a medium with ferrocene as the sole source of carbon. Further confirmation of degradation of ferrocene and its derivatives was done using Gas Chromatography Mass Spectroscopy. Since the bacterium degraded organometallic compounds and released the metal in liquid medium, it could be suggested that this organism can also be used for extracting metal ions from organo-metal containing wastes.Increasing environmental concerns, federal restrictions and bans on the release of organic pollutants in the environment necessitates development of efficient waste treatment methods. While chemical methods of treating wastes might serve the purpose at some level the problem may still persist regarding how to dispose off the treated waste and chemical by-products. As a result, new approaches, including bioremediation, are coming in focus. Bioremediation utilizes the metabolic versatility of microorganisms to degrade hazardous pollutants [1,2]. The indigenous microorganisms could be simulated or specially developed, to be added to the site to degrade, transform, or attenuate organic and organometallic compounds to low levels and nontoxic products [1][2][3].Heavy metal pollution is becoming a major concern as the use of heavy metals is increasing in industrial applications. A number of studies are now available which focus on the bioremediation of toxic metals, which either remove the metals from the waste by bioadsorption [4-6] or convert the chemical properties of the metals by reduction [7,8]. These methods, however, may not be sufficient for treating wastes with organometallic compounds where biodegradation of organic matter is essential. Unlike many other organic pollutants, organometallic pollutants are highly stable as they have stable metallic bonds [9,10]. Thus, bioremediation of organometallic pollutants may require the capacity of microorganisms to break these strong metallic bonds. Farbiszewska-Kiczma and Farbiszewska [11] used phthalocyanines with different metals to isolate bacteria that can degrade organometallic compounds. However, metallic bonds in phthalocyanines are relatively weaker than many other organometallic bonds. Hence, in this study, one of the most stable organometallic compounds, called fer...

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Bioremediation of 60Co from simulated spent decontamination solutions

Cited by 16 publications

References 15 publications

Engineered Deinococcus radiodurans R1 with NiCoT genes for bioremoval of trace cobalt from spent decontamination solutions of nuclear power reactors

Engineered Deinococcus radiodurans R1 with NiCoT genes for bioremoval of trace cobalt from spent decontamination solutions of nuclear power reactors

Cobalt(II) bioaccumulation and distribution in Rhodopseudomonas palustris

Bioremediation of Organometallic Compounds by Bacterial Degradation

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