Bioremediation of acid mine water using facultatively methylotrophic metal-tolerant sulfate-reducing bacteria

Hard, Barbara C.; Friedrich, Stuart; Babel, Wolfgang

doi:10.1016/s0944-5013(97)80025-0

Cited by 53 publications

(29 citation statements)

References 18 publications

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“…A common choice for bioremediation of heavy metals that readily precipitate as sulfides has been the use of SRB to generate hydrogen sulfide from sulfate and to precipitate metals as insoluble metal sulfides (10,12,24).…”

Section: Discussionmentioning

confidence: 99%

Engineering Hydrogen Sulfide Production and Cadmium Removal by Expression of the Thiosulfate Reductase Gene ( phsABC ) from Salmonella enterica Serovar Typhimurium in Escherichia coli

Bang

Clark

Keasling

2000

Appl Environ Microbiol

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The thiosulfate reductase gene (phsABC) from Salmonella enterica serovar Typhimurium was expressed in Escherichia coli to overproduce hydrogen sulfide from thiosulfate for heavy metal removal (or precipitation). A 5.1-kb DNA fragment containing phsABC was inserted into the pMB1-based, high-copy, isopropyl-␤-D-thiogalactopyranoside-inducible expression vector pTrc99A and the RK2-based, medium-copy, m-toluate-inducible expression vector pJB866, resulting in plasmids pSB74 and pSB77. A 3.7-kb DNA fragment, excluding putative promoter and regulatory regions, was inserted into the same vectors, making plasmids pSB103 and pSB107. E. coli DH5␣ strains harboring the phsABC constructs showed higher thiosulfate reductase activity and produced significantly more sulfide than the control strains under both aerobic and anaerobic conditions. Among the four phsABC constructs, E. coli DH5␣ (pSB74) produced thiosulfate reductase at the highest level and removed the most cadmium from solution under anaerobic conditions: 98% of all concentrations up to 150 M and 91% of 200 M. In contrast, a negative control did not produce any measurable sulfide and removed very little cadmium from solution. Energy-dispersive X-ray spectroscopy revealed that the metal removed from solution precipitated as a complex of cadmium and sulfur, most likely cadmium sulfide.Heavy metals are commonly found at many hazardous waste sites in industrialized countries. Many soluble metals can form insoluble complexes with hydroxides, carbonates, phosphates, and sulfides (21). One of the best-known natural metal precipitation mechanisms is due to sulfide production from sulfate by sulfate-reducing bacteria (SRB) found in anoxic sediments containing high concentrations of lead and mercury (9). A recent bioremediation technology utilizes hydrogen sulfide generated by SRB in anaerobic bioreactors to precipitate soluble metal species in aqueous waste streams as insoluble metal sulfides (25). The primary focus of this study was to develop a genetically engineered bacterium capable of producing sulfide under aerobic, microaerobic, or anaerobic conditions for heavy metal precipitation.Among several bacterial hydrogen sulfide-generating systems, we chose the thiosulfate reductase gene (phsABC; phs represents production of hydrogen sulfide) from Salmonella enterica serovar Typhimurium to overproduce hydrogen sulfide. Thiosulfate reduction is a common but incompletely understood feature among bacteria (17). Thiosulfate reductase catalyzes the dissimilatory reduction of inorganic thiosulfate to hydrogen sulfide and sulfite (6). The enzyme has been purified from Desulfovibrio vulgaris (1), D. gigas (13), and a thermophilic iron-oxidizing bacterium, strain TI-1 (22).Mutant and biochemical tests suggested that thiosulfate reductase activity from S. enterica serovar Typhimurium has an absolute requirement for the F 0 F 1 -ATP synthase (20). Sequence analyses of the chromosomal phsABC region from S. enterica serovar Typhimurium revealed a functional operon with three open readin...

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Section: Discussionmentioning

confidence: 99%

Engineering Hydrogen Sulfide Production and Cadmium Removal by Expression of the Thiosulfate Reductase Gene ( phsABC ) from Salmonella enterica Serovar Typhimurium in Escherichia coli

Bang

Clark

Keasling

2000

Appl Environ Microbiol

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“…was present (Fortin et al 1994). Different SRB strains were tested and some showed resistance to concentrations of 50 mM of Al, 30 mM of Cr and/or 10 mM of Pb (Hard et al 1997 (Hao 2000). In comparison to methanogens, SRB appear to be resistant to higher concentrations of metals.…”

Section: Effects Of Heavy Metals On Srbmentioning

confidence: 99%

Methanogens, sulphate and heavy metals: a complex system

Paulo

Stams

Sousa

2015

Rev Environ Sci Biotechnol

125

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Anaerobic digestion (AD) is a well-established technology used for the treatment of wastes and wastewaters with high organic content. During AD organic matter is converted stepwise to methanecontaining biogas-a renewable energy carrier. Methane production occurs in the last AD step and relies on methanogens, which are rather sensitive to some contaminants commonly found in wastewaters (e.g. heavy metals), or easily outcompeted by other groups of microorganisms (e.g. sulphate reducing bacteria, SRB). This review gives an overview of previous research and pilot-scale studies that shed some light on the effects of sulphate and heavy metals on methanogenesis. Despite the numerous studies on this subject, comparison is not always possible due to differences in the experimental conditions used and parameters explained. An overview of the possible benefits of methanogens and SRB co-habitation is also covered. Small amounts of sulphide produced by SRB can precipitate with metals, neutralising the negative effects of sulphide accumulation and free heavy metals on methanogenesis. Knowledge on how to untangle and balance sulphate reduction and methanogenesis is crucial to take advantage of the potential for the utilisation of biogenic sulphide as a metal detoxification agent with minimal loss in methane production in anaerobic digesters.

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“…Demgegenüber existiert eine Reihe von verschiedenen biochemischen Prozessen, die auf Basis der anaeroben Sulfatreduktion [1 -4] Es wurden bisher verschiedene Substrate wie Buttersäure [2], Lactat [5,6], Methanol [7], Ethanol [3,8,9], Pepton, Glucose und Melasse [10,11], Wasserstoff [12,13], Acetat [14], Kompost [15] Während des gesamten Prozesses lag der pH-Wert stets bei oder über 7. Bei einem pHWert von pH ≥7 bildet sich nach [22] Thiosulfat gemäß der Gleichung…”

Section: Introductionunclassified

Kinetics of Anaerobic Biodegradation of Glycerol by Sulfate‐Reducing Bacteria

Dinkel

Frechen

Kljavlin³

2010

Chemie Ingenieur Technik

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Kinetics of Anaerobic Biodegradation of Glycerol by Sulfate-Reducing BacteriaThe efficiency of the production of Biosulfid for the elimination of heavy metals or sulphate from industrial wastewater is crucial on the used substrate and is directly related to the recoverability of the carbon source by microorganisms. Easily accessible and easy to use is glycerol as substrate for the sulfate-reducing bacteria (SRB). Subject of the presented work was the investigation of the anaerobic degradation of glycerol in the batch fermentation. A mixed culture of SRB and a kinetic model approach was determined.

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Bioremediation of acid mine water using facultatively methylotrophic metal-tolerant sulfate-reducing bacteria

Cited by 53 publications

References 18 publications

Engineering Hydrogen Sulfide Production and Cadmium Removal by Expression of the Thiosulfate Reductase Gene ( phsABC ) from Salmonella enterica Serovar Typhimurium in Escherichia coli

Engineering Hydrogen Sulfide Production and Cadmium Removal by Expression of the Thiosulfate Reductase Gene ( phsABC ) from Salmonella enterica Serovar Typhimurium in Escherichia coli

Methanogens, sulphate and heavy metals: a complex system

Kinetics of Anaerobic Biodegradation of Glycerol by Sulfate‐Reducing Bacteria

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