Bioremediation of Chlorinated Compounds

Inobeme, Abel; Adetunji, Charles Oluwaseun; Tsado, Mathew John; Ajai, Alexander Ikechukwu; Inobeme, Jonathan; Oyedolapo, Bamigboye

doi:10.1007/978-3-031-24086-7_5

Modern Approaches in Waste Bioremediation 2023

DOI: 10.1007/978-3-031-24086-7_5

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Bioremediation of Chlorinated Compounds

Abel Inobeme

Charles Oluwaseun Adetunji²,

Mathew John Tsado³

et al.

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2023

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Cited by 1 publication

References 26 publications

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Deciphering Enzyme Mechanisms with Engineered Ancestors and Substrate Analogues

Gao,

Damborsky,

Janin

et al. 2023

ChemCatChem

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Environmentally friendly industrial and biotech processes greatly benefit from enzyme‐based technologies. Their use is often possible only when the enzyme‐catalytic mechanism is thoroughly known. Thus, atomic‐level knowledge of a Michaelis enzyme‐substrate complex, revealing molecular details of substrate recognition and catalytic chemistry, is crucial for understanding and then rationally extending or improving enzyme‐catalyzed reactions. However, many known enzymes sample huge protein conformational space, often preventing complete structural characterization by X‐ray crystallography. Moreover, using a cognate substrate is problematic since its conversion into a reaction product in the presence of the enzyme will prevent the capture of the enzyme‐substrate conformation in an activated state. Here, we outlined how to deal with such obstacles, focusing on the recent discovery of a Renilla‐type bioluminescence reaction mechanism facilitated by a combination of engineered ancestral enzyme and the availability of a non‐oxidizable luciferin analogue. The automated ancestral sequence reconstructions using FireProtASR provided a thermostable enzyme suited for structural studies, and a stable luciferin analogue azacoelenterazine provided a structurally cognate chemical incapable of catalyzed oxidation. We suggest that an analogous strategy can be applied to various enzymes with unknown catalytic mechanisms and poor crystallizability.

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Deciphering Enzyme Mechanisms with Engineered Ancestors and Substrate Analogues

Gao,

Damborsky,

Janin

et al. 2023

ChemCatChem

View full text Add to dashboard Cite

show abstract

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Bioremediation of Chlorinated Compounds

Cited by 1 publication

References 26 publications

Deciphering Enzyme Mechanisms with Engineered Ancestors and Substrate Analogues

Deciphering Enzyme Mechanisms with Engineered Ancestors and Substrate Analogues

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