Bioremediation of chromate: thermodynamic analysis of the effects of Cr(VI) on sulfate-reducing bacteria

Chardin, B.; Dolla, A.; Chaspoul, Florence; Ml, Fardeau; Gallice, Philippe; Bruschi, Mireille

doi:10.1007/s00253-002-1091-8

Cited by 85 publications

(9 citation statements)

References 39 publications

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“…Despite the undesirable features of this metabolic end product, SRB have been exploited in studies of heavy metal bioremediation (Jiang and Fan, 2008; Martins et al, 2009) because of the ability of sulfide to form insoluble complexes with heavy metals (Jalali and Baldwin, 2000). SRB also precipitate heavy metals by directly changing the metal redox state to a less soluble form (Lovley et al, 1993a,b; Lloyd et al, 1999; Chardin et al, 2003). The metabolism of SRB is studied, therefore, to understand how to minimize the detrimental economic effects of these bacteria and to maximize their positive metabolic traits.…”

Section: Introductionmentioning

confidence: 99%

Genetic basis for nitrate resistance in Desulfovibrio strains

Korte

Fels²,

Christensen³

et al. 2014

Front. Microbiol.

202

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Nitrate is an inhibitor of sulfate-reducing bacteria (SRB). In petroleum production sites, amendments of nitrate and nitrite are used to prevent SRB production of sulfide that causes souring of oil wells. A better understanding of nitrate stress responses in the model SRB, Desulfovibrio vulgaris Hildenborough and Desulfovibrio alaskensis G20, will strengthen predictions of environmental outcomes of nitrate application. Nitrate inhibition of SRB has historically been considered to result from the generation of small amounts of nitrite, to which SRB are quite sensitive. Here we explored the possibility that nitrate might inhibit SRB by a mechanism other than through nitrite inhibition. We found that nitrate-stressed D. vulgaris cultures grown in lactate-sulfate conditions eventually grew in the presence of high concentrations of nitrate, and their resistance continued through several subcultures. Nitrate consumption was not detected over the course of the experiment, suggesting adaptation to nitrate. With high-throughput genetic approaches employing TnLE-seq for D. vulgaris and a pooled mutant library of D. alaskensis, we determined the fitness of many transposon mutants of both organisms in nitrate stress conditions. We found that several mutants, including homologs present in both strains, had a greatly increased ability to grow in the presence of nitrate but not nitrite. The mutated genes conferring nitrate resistance included the gene encoding the putative Rex transcriptional regulator (DVU0916/Dde_2702), as well as a cluster of genes (DVU0251-DVU0245/Dde_0597-Dde_0605) that is poorly annotated. Follow-up studies with individual D. vulgaris transposon and deletion mutants confirmed high-throughput results. We conclude that, in D. vulgaris and D. alaskensis, nitrate resistance in wild-type cultures is likely conferred by spontaneous mutations. Furthermore, the mechanisms that confer nitrate resistance may be different from those that confer nitrite resistance.

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Section: Introductionmentioning

confidence: 99%

Genetic basis for nitrate resistance in Desulfovibrio strains

Korte

Fels²,

Christensen³

et al. 2014

Front. Microbiol.

202

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“…However, at higher aeration level the Cr(VI) reduction started to decline. Chromate reduction has been reported by both aerobic (Desai et al 2008a;Poopal and Laxman, 2009) and anaerobic bacteria (Michel et al 2001;Chardin et al 2002). In the presence of oxygen, bacterial Cr 6+ reduction commonly occurs as a two-or three-step process with Cr 6+ initially reduced to the shortlived intermediates Cr 5+ and/or Cr 4+ before further reduction to the thermodynamically stable end product, Cr 3+ (Cheung and Gu, 2007).Cr 3+ (Cheung and Gu, 2007).…”

Section: Fig 5 Effect Of Aeration Level (Shaking Rpm) On the Growth mentioning

confidence: 99%

Isolation and characterization of novel potent Cr(VI) reducing alkaliphilic Amphibacillus sp. KSUCr3 from hypersaline soda lakes

Ibrahim¹,

El-Tayeb²,

Elbadawi³

et al. 2011

Electro Journal of Biotech

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A strain KSUCr3 with extremely high Cr(VI)-reducing ability under alkaline conditions was isolated from hypersaline soda lakes and identified as Amphibacillus sp. on the basis of 16S rRNA gene sequence analysis. The results showed that Amphibacillus sp. strain KSUCr3 was tolerance to very high Cr(VI) concentration (75 mM) in addition to high tolerance to other heavy metals including Ni 2+ (100 mM), Mo 2+ (75 mM), Co 2+ (5 mM), Mn 2+ (100 mM), Zn 2+ (2 mM), Cu 2+ (2 mM) and Pb (75 mM). Strain KSUCr3 was shown to be of a high efficiency in detoxifying chromate, as it could rapidly reduce 5 mM of Cr(VI) to a non detectable level over 24 hrs. In addition, strain KSUCr3 could reduce Cr(VI) efficiently over a wide range of initial Cr(VI) concentrations (1-10 mM) in alkaline medium under aerobic conditions without significant effect on the bacterial growth. Addition of glucose, NaCl and Na 2 CO 3 to the culture medium caused a dramatic increase in Cr(VI)-reduction by Amphibacillus sp. strain KSUCr3. The maximum chromate removal was exhibited in alkaline medium containing 1.5% Na 2 CO 3 , 0.8% glucose, and 1.2% NaCl, at incubation temperature of 40ºC and shaking of 100 rpm. Under optimum Cr(VI) reduction conditions, Cr(VI) reduction rate reached 237 µMh 1 which is one of the highest Cr(VI) reduction rate, under alkaline conditions and high salt concentration, compared to other microorganisms that has been reported so far. Furthermore, the presence of other metals, such as Ni 2+ , Co 2+ , Cu 2+ and Mn 2+ slightly stimulated Cr(VI)-reduction ability by the strain KSUCr3.The isolate, Amphibacillus sp. strain KSUCr3, exhibited an ability to repeatedly reduce hexavalent chromium without any amendment of nutrients, suggesting its potential application in continuous bioremediation of Cr(VI). The results also revealed the possible isolation of potent heavy metals resistant bacteria from extreme environment such as hypersaline soda lakes.

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“…In fact, it is common practice to stimulate growth of existing microorganisms by injecting an excess electron donor into the subsurface, creating an electron acceptor limited environment. Previous studies have reported that substrate limitation can increase Cr(VI) susceptibility (Chardin et al 2002 ), and injections of electron donor without a corresponding increase in electron acceptor creates unbalanced electron donor to acceptor ratios. However, little is known about the physiological responses of metal-reducing populations in the context of unbalanced ratios that occur as a consequence of stimulated conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Desulfovibrio species are model sulfate-reducing bacteria (SRB) and have been shown to reduce metals, metalloids, and radionuclides (Heidelberg et al 2004 ; Klonowska et al 2008 ; Zhou et al 2014 ). Previous work with purified enzymes and whole cells has shown that Desulfovibrio vulgaris is capable of reducing Cr(VI) via hydrogenases and cytochrome c3, but cells are unable to use Cr(VI) as a terminal electron acceptor linked to growth (Lovley and Phillips 1994 ; Chardin et al 2002 ; Elias et al 2004 ). One promising approach for treatment of contaminated environments is in situ biostimulation, the process of promoting indigenous microbial activity via the addition of carbon, nitrogen, phosphorus, and/or energy sources (Tyagi et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Cr(VI) reduction and physiological toxicity are impacted by resource ratio in Desulfovibrio vulgaris

Franco

Steinbeisser

Zane

et al. 2018

Appl Microbiol Biotechnol

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Desulfovibrio spp. are capable of heavy metal reduction and are well-studied systems for understanding metal fate and transport in anaerobic environments. Desulfovibrio vulgaris Hildenborough was grown under environmentally relevant conditions (i.e., temperature, nutrient limitation) to elucidate the impacts on Cr(VI) reduction on cellular physiology. Growth at 20 °C was slower than 30 °C and the presence of 50 μM Cr(VI) caused extended lag times for all conditions, but once growth resumed the growth rate was similar to that without Cr(VI). Cr(VI) reduction rates were greatly diminished at 20 °C for both 50 and 100 μM Cr(VI), particularly for the electron acceptor limited (EAL) condition in which Cr(VI) reduction was much slower, the growth lag much longer (200 h), and viability decreased compared to balanced (BAL) and electron donor limited (EDL) conditions. When sulfate levels were increased in the presence of Cr(VI), cellular responses improved via a shorter lag time to growth. Similar results were observed between the different resource (donor/acceptor) ratio conditions when the sulfate levels were normalized (10 mM), and these results indicated that resource ratio (donor/acceptor) impacted D. vulgaris response to Cr(VI) and not merely sulfate limitation. The results suggest that temperature and resource ratios greatly impacted the extent of Cr(VI) toxicity, Cr(VI) reduction, and the subsequent cellular health via Cr(VI) influx and overall metabolic rate. The results also emphasized the need to perform experiments at lower temperatures with nutrient limitation to make accurate predictions of heavy metal reduction rates as well as physiological states in the environment.Electronic supplementary materialThe online version of this article (10.1007/s00253-017-8724-4) contains supplementary material, which is available to authorized users.

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Bioremediation of chromate: thermodynamic analysis of the effects of Cr(VI) on sulfate-reducing bacteria

Cited by 85 publications

References 39 publications

Genetic basis for nitrate resistance in Desulfovibrio strains

Genetic basis for nitrate resistance in Desulfovibrio strains

Isolation and characterization of novel potent Cr(VI) reducing alkaliphilic Amphibacillus sp. KSUCr3 from hypersaline soda lakes

Cr(VI) reduction and physiological toxicity are impacted by resource ratio in Desulfovibrio vulgaris

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