Bioremediation of chromium by the yeast Pichia guilliermondii: toxicity and accumulation of Cr (III) and Cr (VI) and the influence of riboflavin on Cr tolerance

Ksheminska, Helena; Jaglarz, Anita; Fedorovych, Daria V.; Babyak, Lyubov; Yanovych, D.; Kaszycki, Paweł; Kołoczek, Henryk

doi:10.1078/0944-5013-00178

Cited by 50 publications

(26 citation statements)

References 40 publications

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“…It is likely that A. parasiticus may have taken up so much Cr(VI) that it could not convert to Cr(III) since intracellular accumulation of Cr(VI) may also disturb the normal functioning of microbial cells [34]. In studies of chromium toxicity and accumulation, Kshminska et al [10] reported diverse, individual and complex mechanism of chromium bioremediation in the cells of different strains of the yeast Pichia guilliermondii. They observed that all the investigated strains responded to Cr(VI) treatment with substantial changes in protein profile patterns, which included both induction and suppression of certain polypeptides.…”

Section: Resultsmentioning

confidence: 99%

“…For the determination of total chromium uptake by the fungi, the mycelia were acid digested [10,32]. The cells were separated from the culture media by filtration, washed with water and dried.…”

Section: Determination Of Total Chromium Uptake By Fungal Biomassmentioning

confidence: 99%

“…Biosorption of chromium from aqueous solutions by fungal pellets of Aspergillus niger has also been reported [13]; The bioremediation of chromium by the yeast Pichia guilliermondii [10] and Saccharomyces cerevisiae [9,19] has also been studied. Increased tolerance to chromium and enhanced Cr accumulation capabilities were observed among Cr-resistant mutants of Pichia guilliermondii obtained by means of environmental mutagenesis [10].…”

Section: Introductionmentioning

confidence: 99%

“…Many species of bacteria [1,[3][4][5][6][7][8], yeasts [9][10][11] and fungi [12][13][14][15][16] have been investigated from this aspect, and the biosorption and bioaccumulation of chromium for bioremediation purposes have been demonstrated. Park et al [13] had demonstrated that Pseudomonas putida culture was able to carry out enzymatic reduction of chromates.…”

Section: Introductionmentioning

confidence: 99%

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Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Shugaba¹,

Buba²,

Bg³

et al. 2012

J Pet Environ Biotechnol

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The uptake and reduction of Cr(VI) by Aspergillus niger and A. parasiticus was studied in this journal. After 96 hours of growth, the culture solutions spiked with an initial dichromate concentration of 20 mg/l, were completely decolorized and had residual Cr(VI) concentrations of only 0.74 ± 0.55 and 1.69 ± 0.29 mg/l in A. niger and A. parasiticus cultures representing Cr(VI) removal of 96.3% and 91.6%, respectively. In the A. niger culture, significantly (P < 0.01) lower Cr(VI) concentrations were observed within 72 hours of growth compared to those of A. parasiticus, but in both cultures complete removal was almost achieved by 144 hours of growth. The rate of Cr(VI) removal was 0.21 ± 0.09 mgl -1 hr -1 and 0.20 ± 0.07 mgl -1 hr -1 for A. niger and A. parasiticus, respectively. Cellular concentrations of Cr(VI) in the two fungi increased significantly (P < 0.05 -0.001) with increasing concentrations of the dichromate treatments. Although tannic acid as sole source of carbon and energy gave significantly lower Cr(VI) removal than glucose (P < 0.001) and acetate (P < 0.01), it supported the removal of about 85.0% and 68.8% of the metal ion by A. niger and A. parasiticus, respectively. The active mycelia of both fungi showed significantly (P < 0.001) higher Cr(VI) removal than inactivated mycelia after incubation at 30°C for 72 hours. Incubation of cell -free extracts of both fungi with NADH at 30°C for 2 hours showed Cr(VI) reduction of 68.0% and 55.5% for A. niger and A. parasiticus, respectively. These findings suggest that uptake and metabolic reduction may be the process by which the two fungi are able to tolerate the toxic effects of hexavalent chromium. However, Cr removal via uptake by the two fungal biomass was observed to be in the range of 0.5 -1.78% only, for all the concentrations applied, which is insignificant when compared with the initial Cr concentration in the culture medium. The results obtained through this investigation indicate the possibility of treating waste effluents containing hexavalent chromium using Aspergillus niger and A. parasiticus.

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Section: Resultsmentioning

confidence: 99%

Section: Determination Of Total Chromium Uptake By Fungal Biomassmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Shugaba¹,

Buba²,

Bg³

et al. 2012

J Pet Environ Biotechnol

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“…The cell-free extract was submitted at 95°C and tested for chromate reduction, but no changes were observed, which demonstrates that one type of enzyme should be conducting the reduction process. Additional studies conducted on C. maltosa (Ramírez et al 2004), Pichia jadinii (Ksheminska et al 2003) and Aspergillus tubingensis Ed8 (Coreno et al 2009) have demonstrated the high specific activity of chromate reductase in crude extract, but not in the membrane fraction.…”

Section: Direct Reductionmentioning

confidence: 99%

Metallophilic fungi research: an alternative for its use in the bioremediation of hexavalent chromium

García-Hernández

Villarreal-Chiu

Garza-González

2017

Int. J. Environ. Sci. Technol.

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Contamination by hexavalent chromium has had a large impact on modern society and human health. This problem is a consequence of its great industrial applicability to several products and processes. Short-term exposure to hexavalent chromium can cause irritation, ulceration in skin and stomach and in addition to cancer, dermatitis, and damage to liver, renal circulation and nervous tissues, with even death being observed in response to long-term exposures. Many techniques have been used for the remediation of this pollutant, including physical and chemical approaches and, in more recent years, biological methods. Filamentous fungi isolated from contaminated sites exhibit a significant tolerance to heavy metal; hence, they are an important source of microbiota capable of eliminating hexavalent chromium from the environment. However, these microorganisms can do so in different ways, including biosorption, bioreduction, and bioaccumulation, among others. In this review, we explore several of the most documented mechanisms that have been described for fungi/hexavalent chromium interactions and their potential use in bioremediation.

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Removal of Cr(VI) from aqueous solution by fungal biomass

Ahluwalia¹,

Goyal²

2010

Engineering in Life Sciences

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Chromium compounds are released by industrial processes including leather production, mining, petroleum refining, in textile industry and dyeing. They are a significant threat to the environment and public health because of their toxicity. Removal of hexavalent chromium by living biomass of different fungi was effective in the order of Aspergillus terricola>Aspergillus niger>Acremonium strictum>Aureobasidium pullulans>Paecilomyces variotii>Aspergillus foetidus>Cladosporium resinae>Phanerochaete chrysosporium. Non‐living dried fungal biomass showed higher potential for metal removal than living cells. Among all fungi dead biomass of P. chrysosporium, C. resinae and P. variotii had the maximum specific chromium uptake capacity, which was 11.02, 10.69 and 10.35 mg/g of dry biomass respectively at pH 4.0–5.0 in batch sorption. Removal of Cr(VI) by P. chrysosporium from multi‐metallic synthetic solution as well as chrome effluent was significant by bringing down the residual concentration to 0.1 mg/L in the effluent, which falls within the permissible range and its removal was not affected by the presence of other metal ions such as Fe, Zn and Ni. Fourier transform infrared spectral analysis revealed the presence of carboxylate (C=O) and amine (−NH, −NH) functional groups commonly present on the cell surface of all fungi, with possible involvement in chromium binding. The result indicates that non‐living fungal biomass either obtained as a by‐product of fermentation industry or mass produced using inexpensive culture media can be used for bioremediation of Cr(VI) from chrome effluent on large scale.

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Bioremediation of chromium by the yeast Pichia guilliermondii: toxicity and accumulation of Cr (III) and Cr (VI) and the influence of riboflavin on Cr tolerance

Cited by 50 publications

References 40 publications

Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Metallophilic fungi research: an alternative for its use in the bioremediation of hexavalent chromium

Removal of Cr(VI) from aqueous solution by fungal biomass

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