Bioremediation of Nitroexplosive Waste Waters

Kanekar, Pradnya Pralhad; Sarnaik, Seema; Dautpure, Premlata Sukhdev; Patil, Vrushali Prashant; Kanekar, Sagar Pralhad

doi:10.1007/978-3-319-01083-0_4

Cited by 10 publications

(6 citation statements)

References 84 publications

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“…Znanych jest kilka konwencjonalnych metod neutralizacji omawianej klasy substancji: (foto-) utlenianie, hydroliza, parowanie, spalanie, adsorpcja itd. [48]. Metody fizykochemiczne posiadają jednak liczne wady i ograniczenia.…”

Section: Biodegradacja Nitrozwiązków Aromatycznychunclassified

“…Ogólna strategia wykorzystywana przez drobnoustroje w celu degradacji nitrozwiązków aromatycznych jest analo giczna do tej stosowanej w oksydacyjnej ścieżce rozkładu aminokwasów i węglowodorów aromatycznych. Polega ona na konwersji substratu do fenoli, chinonów i katecholi, które w dalszej kolejności rozkładane są do intermediatów cyklu kwasów trójkarboksylowych (cykl Krebsa) [48].…”

Section: Degradacja Tlenowa (Aerobowa)unclassified

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Nitroaromatic compounds – characteristics and methods of biodegradation

Wysocka

Olszyna

Komorowska

et al. 2017

Postępy Mikrobiologii - Advancements of Microbiology

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Section: Biodegradacja Nitrozwiązków Aromatycznychunclassified

Section: Degradacja Tlenowa (Aerobowa)unclassified

Nitroaromatic compounds – characteristics and methods of biodegradation

Wysocka

Olszyna

Komorowska

et al. 2017

Postępy Mikrobiologii - Advancements of Microbiology

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“…Especially OYEs´ ability to degrade xenobiotics opens new ways for applications in medicine and biotechnology. For instance, their ene-reductase activity allows an effective, asymmetric hydrogenation of α, β-unsaturated double bonds with a high selectivity and reaction yield [ 11 ], their nitro-reductase activity is useful for bioremediation applications [ 12 ]. Involvement of OYEs in xenobiotics degradation suggests they can be promising drug targets.…”

Section: Introductionmentioning

confidence: 99%

The crystal structure of XdpB, the bacterial old yellow enzyme, in an FMN-free form

et al. 2018

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Old Yellow Enzymes (OYEs) are NAD(P)H dehydrogenases of not fully resolved physiological roles that are widespread among bacteria, plants, and fungi and have a great potential for biotechnological applications. We determined the apo form crystal structure of a member of the OYE class, glycerol trinitrate reductase XdpB, from Agrobacterium bohemicum R89-1 at 2.1 Å resolution. In agreement with the structures of the related bacterial OYEs, the structure revealed the TIM barrel fold with an N-terminal β-hairpin lid, but surprisingly, the structure did not contain its cofactor FMN. Its putative binding site was occupied by a pentapeptide TTSDN from the C-terminus of a symmetry related molecule. Biochemical experiments confirmed a specific concentration-dependent oligomerization and a low FMN content. The blocking of the FMN binding site can exist in vivo and regulates enzyme activity. Our bioinformatic analysis indicated that a similar self-inhibition could be expected in more OYEs which we designated as subgroup OYE C1. This subgroup is widespread among G-bacteria and can be recognized by the conserved sequence GxxDYP in proximity of the C termini. In proteobacteria, the C1 subgroup OYEs are typically coded in one operon with short-chain dehydrogenase. This operon is controlled by the tetR-like transcriptional regulator. OYEs coded in these operons are unlikely to be involved in the oxidative stress response as the other known members of the OYE family because no upregulation of XdpB was observed after exposing A. bohemicum R89-1 to oxidative stress.

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“…Due to its superior physicochemical properties, 2,4,6-trinitrotoluene (TNT) is the most widely used NAC in the industry and hence has the largest contribution to environmental contamination [ 8 , 9 ]. The requirement for clean-up of TNT-polluted regions has motivated development and examination of several remediation processes including composting [ 10 ], incineration [ 11 ], and chemical oxidation [ 12 ]. These methods, however, suffer from one or more drawbacks such as high cost, incomplete degradation, generation of potentially more toxic by-products, and health and safety risks to workers [ 2 , 6 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Systems Biology Approach to Bioremediation of Nitroaromatics: Constraint-Based Analysis of 2,4,6-Trinitrotoluene Biotransformation by Escherichia coli

et al. 2017

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Microbial remediation of nitroaromatic compounds (NACs) is a promising environmentally friendly and cost-effective approach to the removal of these life-threating agents. Escherichia coli (E. coli) has shown remarkable capability for the biotransformation of 2,4,6-trinitro-toluene (TNT). Efforts to develop E. coli as an efficient TNT degrading biocatalyst will benefit from holistic flux-level description of interactions between multiple TNT transforming pathways operating in the strain. To gain such an insight, we extended the genome-scale constraint-based model of E. coli to account for a curated version of major TNT transformation pathways known or evidently hypothesized to be active in E. coli in present of TNT. Using constraint-based analysis (CBA) methods, we then performed several series of in silico experiments to elucidate the contribution of these pathways individually or in combination to the E. coli TNT transformation capacity. Results of our analyses were validated by replicating several experimentally observed TNT degradation phenotypes in E. coli cultures. We further used the extended model to explore the influence of process parameters, including aeration regime, TNT concentration, cell density, and carbon source on TNT degradation efficiency. We also conducted an in silico metabolic engineering study to design a series of E. coli mutants capable of degrading TNT at higher yield compared with the wild-type strain. Our study, therefore, extends the application of CBA to bioremediation of nitroaromatics and demonstrates the usefulness of this approach to inform bioremediation research.

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Bioremediation of Nitroexplosive Waste Waters

Cited by 10 publications

References 84 publications

Nitroaromatic compounds – characteristics and methods of biodegradation

Nitroaromatic compounds – characteristics and methods of biodegradation

The crystal structure of XdpB, the bacterial old yellow enzyme, in an FMN-free form

Systems Biology Approach to Bioremediation of Nitroaromatics: Constraint-Based Analysis of 2,4,6-Trinitrotoluene Biotransformation by Escherichia coli

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