Bioremediation of Phenanthrene by Mycoplana sp. MVMB2 Isolated from Contaminated Soil

Abstract

Bioremediation of Phenanthrene by Mycoplana sp. MVMB2 Isolated from Contaminated SoilThe effect of nutrient and surfactant addition on the biodegradation of phenanthrene was studied in a batch scale soil-slurry system using isolated Mycoplana sp. MVMB2strain. The study was conducted using an artificially phenanthrene spiked and as well as contaminated soil from petrochemical industrial site. Maximum phenanthrene degradation and subsequent high microbial growth were observed at optimum pH (pH 6) and C/N/P ratio (100:20:3). To investigate maximum substrate degradation potential of Mycoplana sp. MVMB2, very high concentrations of phenanthrene (50-200 mg/kg soil) were used. The organism was capable of degrading >60% for a concentration below 20 mg/kg soil and >40% for concentrations up to 200 mg/kg within 8 days. Further the influence of five different surfactants namely Span 80, Tween 20, Triton X-100, cetyl trimethyl ammonium bromide, and sodium dodecyl sulfate were tested at their critical micelle concentration (CMC) levels for phenanthrene degradation in the soil. The addition of surfactant enhanced the biodegradation and a maximum of 84.49% was obtained for Triton X-100. Complete phenanthrene degradation by Mycoplana sp. MVMB2 was observed at 3 CMC concentration of Triton X-100. The optimized parameters obtained were used for the degradation of phenanthrene present in the contaminated soil and 98.6% biodegradation was obtained. Thus, the results obtained in the study suggested that biodegradation of phenanthrene by Mycoplana sp. MVMB2 appeared to be feasible to remediate phenanthrene rich contaminated sites.

Phenanthrene Biodegradation by Pseudomonas xanthomarina Isolated from an Aged Contaminated Soil

“…An indigenous microbial community involved in the degradation of PAHs already exists in contaminated soils and the microorganisms have adapted and acclimatized to PAHs degradation. Several authors reported a wide variety of bacteria capable of metabolizing PAHs including the genera Acidovorax , Acinetobacter , Aeromonas , Alcaligenes , Arthrobacter , Beijerinckia , Brevibacterium , Flavobacterium , Micrococcus , Mycobacterium , Mycoplana , Nocardia , Pseudomonas , Rhodococcus , Sphingomonas , Streptomyces , Vibrio , etc.…”

“…Several authors reported a wide variety of bacteria capable of metabolizing PAHs including the genera Acidovorax , Acinetobacter , Aeromonas , Alcaligenes , Arthrobacter , Beijerinckia , Brevibacterium , Flavobacterium , Micrococcus , Mycobacterium , Mycoplana , Nocardia , Pseudomonas , Rhodococcus , Sphingomonas , Streptomyces , Vibrio , etc. .…”

“…However, a successful bioremediation seems to rely, among other factors, on the selectivity and specialization of the added microorganisms. In this sense, recent studies pointed out the need of using microbes pre‐selected from their own environment to get an effective biological treatment .…”

Succession of microbial populations and nitrogen-fixation associated with the biodegradation of sediment-oil-agglomerates buried in a Florida sandy beach

“…A DNA-based and stable isotope probing (SIP) study with [U- 13 C]anthraquinone from PAH-contaminated soil showed that the genus Phenylobacterium is responsible for anthraquinone degradation 43 . The genus Mycoplana is also known to degrade aromatic hydrocarbons 44,45 . Our observations are corroborated by petroleum hydrocarbon analysis, which revealed that short-chain alkanes (C 15 ) and relatively low-molecular weight PAHs such as naphthalene were rapidly depleted over the first ~100 days when populations of early responders were enriched 32 .

Figure 5Relative abundance of taxa detected at the genus level in SOAs (solid) and uncontaminated control sands (dotted) over the 3-year time course.

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