Bioremediation of Plant Refuges and Xenobiotics

Tiwari, Soni; Tripathi, Ashutosh; Gaur, Rajeeva

doi:10.1007/978-981-10-1866-4_4

Cited by 11 publications

(9 citation statements)

References 44 publications

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“…In this study, the selected bacterium, with the highest tolerance to cyanide, is shown in Fig. 1 (15 mM–976 mg L −1 ), although in the expression of Soni Tiwari, microorganisms cannot tolerate a high concentration of cyanide (Tiwari et al 2017 ). The maximum rate of cyanide degradation was reported to be 700 mg L −1 by (Wu et al 2014 ).…”

Section: Discussionmentioning

confidence: 89%

Significant increase in cyanide degradation by Bacillus sp. M01 PTCC 1908 with response surface methodology optimization

et al. 2017

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Cyanide is used in many industries despite its toxicity. Cyanide biodegradation is affordable and eco-friendly. Sampling from cyanide-contaminated areas from the Muteh gold mine and isolation of 24 bacteria were performed successfully. The selected bacteria—‘Bacillus sp. M01’—showed maximum tolerance (15 mM) to cyanide and deposited in Persian Type Culture Collection by PTCC No.: 1908. In the primary experiments, effective factors were identified through the Plackett–Burman design. In order to attain the maximum degradation by Bacillus sp. M01 PTCC 1908, culture conditions were optimized by using response surface methodology. By optimizing the effective factor values and considering the interaction between them, the culture conditions were optimized. The degradation percentage was calculated using one-way ANOVA vs t test, and was found to have increased 2.35 times compared to pre-optimization. In all of the experiments, R2 was as high as 91%. The results of this study are strongly significant for cyanide biodegradation. This method enables the bacteria to degrade 86% of 10 mM cyanide in 48 h. This process has been patented in Iranian Intellectual Property Centre under Licence No: 90533.

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Section: Discussionmentioning

confidence: 89%

Significant increase in cyanide degradation by Bacillus sp. M01 PTCC 1908 with response surface methodology optimization

et al. 2017

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“…Before the advent of genome sequencing, ketoreduction was typically achieved by isolating organisms from rich physiological sources. Both Medium-Chain Dehydrogenases/Reductases (MDRs) and Short-Chain Dehydrogenases/Reductases (SDRs) play crucial roles in the detoxification of xenobiotics, dyes, and secondary metabolites in nature [18] [19]. They have also been utilized in the degradation of dyes, pharmaceuticals, oil spills, and long-chain alkanes, among others [20].…”

Section: Resultsmentioning

confidence: 99%

Reducing the vicissitudes of heterologous prochiral substrate catalysis by alcohol dehydrogenases through machine learning algorithms

Ghatak,

Shanbhag,

Datta

2024

Biochemical and Biophysical Research Communications

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“…The region that "specifies" the substrate entry and contains the structural zinc domain is depicted in cyan (5). The rest of the residues are depicted in grey (6).…”

Section: Engineering Techniques For Manipulating Ketoreductasesmentioning

confidence: 99%

“…In nature, MDRs and SDRs participate in detoxifying xenobiotics, dyes and producing secondary metabolites. Studies also report upregulation in SDRs in dye and pharmaceutical degrading microbes [5,6] . Further, they are used for making catalytic cocktails to degrade oil spills, long‐chain alkanes, etc.…”

Section: Introductionmentioning

confidence: 99%

“…Studies also report upregulation in SDRs in dye and pharmaceutical degrading microbes. [5,6] Further, they are used for making catalytic cocktails to degrade oil spills, long-chain alkanes, etc.. [7] Therefore, organisms that grow on decaying wood, rotten fruit, wastewater, or toxic landfills have been good sources for exploring members from both superfamilies.…”

Section: Introductionmentioning

confidence: 99%

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Stairway to Stereoisomers: Engineering Short‐ and Medium‐Chain Ketoreductases To Produce Chiral Alcohols

Shanbhag

2023

ChemBioChem

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The short-and medium-chain dehydrogenase/reductase superfamilies are responsible for most chiral alcohol production in laboratories and industries. In nature, they participate in diverse roles such as detoxification, housekeeping, secondary metabolite production, and catalysis of several chemicals with commercial and environmental significance. As a result, they are used in industries to create biopolymers, active pharmaceutical intermediates (APIs), and are also used as components of modular enzymes like polyketide synthases for fabricating bioactive molecules. Consequently, random, semi-rational and rational engineering have helped transform these enzymes into product-oriented efficient catalysts. The rise of newer synthetic chemicals and their enantiopure counterparts has proved challenging, and engineering them has been the subject of numerous studies. However, they are frequently limited to the synthesis of a single chiral alcohol. The study attempts to defragment and describe hotspots of engineering short-and medium-chain dehydrogenases/reductases for the production of chiral synthons.

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Bioremediation of Plant Refuges and Xenobiotics

Cited by 11 publications

References 44 publications

Significant increase in cyanide degradation by Bacillus sp. M01 PTCC 1908 with response surface methodology optimization

Significant increase in cyanide degradation by Bacillus sp. M01 PTCC 1908 with response surface methodology optimization

Reducing the vicissitudes of heterologous prochiral substrate catalysis by alcohol dehydrogenases through machine learning algorithms

Stairway to Stereoisomers: Engineering Short‐ and Medium‐Chain Ketoreductases To Produce Chiral Alcohols

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