Bioremediation of Textile Azo Dyes by Marine Streptomyces

Chakravarthi, Bhoodevi; Mathakala, Vani; Palempalli, Uma Maheswari Devi

doi:10.1007/978-981-15-0706-9_13

Cited by 7 publications

(2 citation statements)

References 11 publications

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“…After cleavage, the products are subjected to oxidative cleavage desulfonation, deamination, demethylation and dehydroxylation, depending on the azo dye structure 48,14 . Research reports demonstrated the degradation through non-specific free-radical mechanism without the cleavage of azo bonds 50,34 . The Mass spectral data of Congo red -21 degraded by laccase is in agreement with literature reported the asymmetric cleavage of the azo bond 52 .…”

Section: Congo Red Dye Degradation By Immobilized Laccasementioning

confidence: 99%

Degradation and Detoxification of Congo Red azo dye by Immobilized Laccase of Streptomyces sviceus

Chakravarthi¹,

Mathkala²,

Palempalli³

2021

J Pure Appl Microbiol

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The discharge of textile effluents enriched with reactive azo dyes is of critical importance owing to inability of the dyes to degrade in waste water and their carcinogenic, mutagenic effects to various organisms. This study initiated based on the need to gaze into molecular mechanism of marine bacterial bioremediation process to develop strategies for the decolorization and detoxification of the synthetic azo dyes. The experimental work carried out to explore decolorization and degradation efficacy of laccase derived from marine actinobacteria, Streptomyces sviceus by choosing Congo red-21 as model azo dye. The extracellular production of laccase was confirmed with plate assay in medium supplemented with ABTS as substrate. Laccase was purified to homogeneity from 72hrs culture of Streptomyces sviceus by Fast performance liquid chromatography and the molecular size of laccase was noticed as 60 kDa. The purified laccase was immobilized with an efficiency of 82% by Calcium alginate method. The crude, purified and immobilized forms of the laccase enzyme was used to decolorize the Congo red-21. Crude laccase enzyme showed 69% of decolorization of Congo red-21 after 48h where as purified and immobilized laccase represented 78% and 92% of colour removal after 24 h respectively. Fourier-transform infrared spectroscopy, High Performance Liquid Chromatography and Gas chromatography–mass spectrometry were used to unravel the molecular mechanism of dye detoxification and also identify nontoxic products released from Congo Red-21 upon administration with immobilized laccase. Based on GC-MS data, it may deduce that immobilized laccase of Streptomyces sviceus cleaves the Congo red-21 dye followed by oxidative cleavage, desulfonation, deamination, demethylation process.

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Section: Congo Red Dye Degradation By Immobilized Laccasementioning

confidence: 99%

Degradation and Detoxification of Congo Red azo dye by Immobilized Laccase of Streptomyces sviceus

Chakravarthi¹,

Mathkala²,

Palempalli³

2021

J Pure Appl Microbiol

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“…Every year a huge number of aquatic organisms die due to dye-laden wastewater and humans may cause allergies, skin irritations, nausea, hemorrhage, etc. Some dyes are carcinogenic to humans causing skin cancers which is very scary for the future [10][11][12][13]. One such dye that is commonly used in research and laboratory is methyl red dye which is ultimately poured into the sewer system.…”

Section: Introductionmentioning

confidence: 99%

Efficient degradation of methyl red dye from the aqueous solution by individual bacterial and their consortium in a sugarcane bagasse waste-based media

Patel,

Desai,

Yadav

et al. 2024

Environ. Res. Commun.

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Dye is one of the major pollutions around the whole world which have adverse effects on the environment and human beings. There is a requirement for an efficient, reliable, sustainable, and eco-friendly approach to the removal of dyes. In the present research, three individual bacterial strains Enterobacter aerogenes (MBX6), Klebsiella pneumoniae (MBC34), and Micrococcus luteus (MBC23) and their consortium was used to remove Methyl Red (MR) dye. The dye removal was observed at different initial concentrations of dye, nutrient media, additives in the nutrient media and sugarcane bagasse extract as a bacterial growth medium. The outcomes of the study showed that the bacterial consortium exhibits better removal efficiencies (63.6%) than individual bacterial strains and the dye removal percentage (75.19%) enhanced with increasing concentration of sucrose (3%) which was used as media additive. The addition of the sugar in nutrient medium resulted in more effective dye removal. The maximum removal of MR dye (98.6%) was achieved when the consortium was grown in a media containing pure sugarcane bagasse extract. The use of sugarcane bagasse extract (agricultural waste product) as a novel growth medium for the cultivation of bacteria possessing dye removal capabilities can offer a sustainable and cost-effective solution for industrial wastewater treatment.

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Functional Annotation and Biotechnological Applications of Soil Microbiomes: Current Research and Future Challenges

Yadav

Kaur

Kour

et al. 2021

Sustainable Development and Biodiversity

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Bioremediation of Textile Azo Dyes by Marine Streptomyces

Cited by 7 publications

References 11 publications

Degradation and Detoxification of Congo Red azo dye by Immobilized Laccase of Streptomyces sviceus

Degradation and Detoxification of Congo Red azo dye by Immobilized Laccase of Streptomyces sviceus

Efficient degradation of methyl red dye from the aqueous solution by individual bacterial and their consortium in a sugarcane bagasse waste-based media

Functional Annotation and Biotechnological Applications of Soil Microbiomes: Current Research and Future Challenges

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