Bioremediation of Zinc Using Bacillus sp. Isolated from Metal-Contaminated Industrial Zone

Krishna, M. P.; Varghese, Rosemol; Babu, Vikash; Jyothy, S.; Hatha, A. A. Mohamed

doi:10.1007/978-81-322-0810-5_2

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Biosorption potential of ZTB strains showing higher MIC was determined by atomic absorption spectroscopy (AAS) as the amount of metal present in the supernatant after the treatment with ZTB strains 45 . Nutrient broth supplemented with Zn was inoculated with the 1% of overnight grown ZTB isolate and incubated for 72 h in a shaking condition.…”

Section: Methodsmentioning

confidence: 99%

Zinc tolerant plant growth promoting bacteria alleviates phytotoxic effects of zinc on maize through zinc immobilization

Jain

Kour

Bhojiya

et al. 2020

Sci Rep

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the increasing heavy metal contamination in agricultural soils has become a serious concern across the globe. The present study envisages developing microbial inoculant approach for agriculture in Zn contaminated soils. Potential zinc tolerant bacteria (ZTB) were isolated from zinc (Zn) contaminated soils of southern Rajasthan, India. Isolates were further screened based on their efficiency towards Zn tolerance and plant growth promoting activities. Four strains viz. ZTB15, ZTB24, ZTB28 and ZTB29 exhibited high degree of tolerance to Zn up to 62.5 mM. The Zn accumulation by these bacterial strains was also evidenced by AAS and SEM-EDS studies. Assessment of various plant growth promotion traits viz., iAA, GA 3 , nH 3 , Hcn, siderophores, Acc deaminase, phytase production and p, K, Si solubilization studies revealed that these ZTB strains may serve as an efficient plant growth promoter under in vitro conditions. Gluconic acid secreted by ZTB strains owing to mineral solubilization was therefore confirmed using high performance liquid chromatography. A pot experiment under Zn stress conditions was performed using maize (Zea mays) variety (FEM-2) as a test crop. Zn toxicity reduced various plant growth parameters; however, inoculation of ZTB strains alleviated the Zn toxicity and enhanced the plant growth parameters. The effects of Zn stress on antioxidant enzyme activities in maize under in vitro conditions were also investigated. An increase in superoxide dismutase, peroxidase, phenylalanine ammonia lyase, catalase and polyphenol oxidase activity was observed on inoculation of ZTB strains. Further, ZIP gene expression studies revealed high expression in the ZIP metal transporter genes which were declined in the ZTB treated maize plantlets. The findings from the present study revealed that ZtB could play an important role in bioremediation in Zn contaminated soils.

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Section: Methodsmentioning

confidence: 99%

Zinc tolerant plant growth promoting bacteria alleviates phytotoxic effects of zinc on maize through zinc immobilization

Jain

Kour

Bhojiya

et al. 2020

Sci Rep

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“…1). Krishna et al [9] also recorded 25% of Zn reduction at neutral pH (pH 7). However, the reductions appear to be in close range across the treatments, yet the variations could be significantly traced to the individual isolates used as inoculants.…”

Section: Resultsmentioning

confidence: 91%

Effect of Mixing Individual Isolates for Bioreduction of Metals in Contaminated Soil

Emenike¹,

Izyani²,

Fauziah³

2017

IJBBB

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Bioremediation of metal contaminated soil involves complex processes, and the non-degradable nature of metals makes it more difficult. The known sources of metal pollution are many and adopting a specific remediation procedure for it is often impossible. Microbes found at polluted sites tend to serve as good remediation agents due to perceived environmental potential. Therefore, it is imperative to select bioremediation by enhancing microbes potential as a way of restoring metal polluted site to original or undisturbed state. Hence, this study tried to evaluate the individual effect of bacterial isolates on the remediation of metal polluted soil from landfill, against combined impact of microbes blended together. Soil samples that characterized of Cd 2+ , Cr 2+ , Pb 2+ and Zn 2+ were subjected to bioremediation. Results revealed no more than 50% reduction across the treatments amended with individual isolates. Rather higher metal remediation was observed when individual isolates were mixed prior to application. Therefore, the study suggests that individual isolates from contaminated sites can selectively metabolize to bioreduce metal concentrations. However, a mix of such isolates with manipulation in the concentration level can enhance metal reduction efficiency (>50%).

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“…This ability of Bacillus spp. is regulated either by acquiring resistance through plasmids or by evolving mechanisms of resistance [ 208 , 209 ]. In B. subtilis , Zn uptake is regulated by the Zur family, which enables the transport of Zn ions via two transporter proteins.…”

Section: Bacillus Species and Heavy Metalsmentioning

confidence: 99%

Unraveling the Underlying Heavy Metal Detoxification Mechanisms of Bacillus Species

2021

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The rise of anthropogenic activities has resulted in the increasing release of various contaminants into the environment, jeopardizing fragile ecosystems in the process. Heavy metals are one of the major pollutants that contribute to the escalating problem of environmental pollution, being primarily introduced in sensitive ecological habitats through industrial effluents, wastewater, as well as sewage of various industries. Where heavy metals like zinc, copper, manganese, and nickel serve key roles in regulating different biological processes in living systems, many heavy metals can be toxic even at low concentrations, such as mercury, arsenic, cadmium, chromium, and lead, and can accumulate in intricate food chains resulting in health concerns. Over the years, many physical and chemical methods of heavy metal removal have essentially been investigated, but their disadvantages like the generation of chemical waste, complex downstream processing, and the uneconomical cost of both methods, have rendered them inefficient,. Since then, microbial bioremediation, particularly the use of bacteria, has gained attention due to the feasibility and efficiency of using them in removing heavy metals from contaminated environments. Bacteria have several methods of processing heavy metals through general resistance mechanisms, biosorption, adsorption, and efflux mechanisms. Bacillus spp. are model Gram-positive bacteria that have been studied extensively for their biosorption abilities and molecular mechanisms that enable their survival as well as their ability to remove and detoxify heavy metals. This review aims to highlight the molecular methods of Bacillus spp. in removing various heavy metals ions from contaminated environments.

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Bioremediation of Zinc Using Bacillus sp. Isolated from Metal-Contaminated Industrial Zone

Cited by 6 publications

References 23 publications

Zinc tolerant plant growth promoting bacteria alleviates phytotoxic effects of zinc on maize through zinc immobilization

Zinc tolerant plant growth promoting bacteria alleviates phytotoxic effects of zinc on maize through zinc immobilization

Effect of Mixing Individual Isolates for Bioreduction of Metals in Contaminated Soil

Unraveling the Underlying Heavy Metal Detoxification Mechanisms of Bacillus Species

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