Bioremediation: Plasmid-Mediated Bioaugmentation of Microbial Communities – Experience from Laboratory-Scale Bioreactors

Hausner, Martina; Stárek, M; Bathe, Stephan

doi:10.1016/b978-0-444-53199-5.00065-8

“…Since this early work, several investigations have been dedicated in testing this concept in wastewater treatment, and so far, most of this work has been carried out at laboratory scales (and to a lesser extent, at pilot scale). Readers should refer to the following excellent reviews on this topic [ 96 , 97 ]. Overall, contradictory results have been obtained, some experiments proving the efficacy of this method while others not [ 96 , 97 ].…”

Section: Potential Strategy To Improve the Efficiency Of Bioaugmenmentioning

confidence: 99%

“…Readers should refer to the following excellent reviews on this topic [ 96 , 97 ]. Overall, contradictory results have been obtained, some experiments proving the efficacy of this method while others not [ 96 , 97 ]. The following have been the proffered main causes of failure: inability of donor bacteria to persist in the environment, inefficiency of plasmid transfer in recipient bacteria, low number of donor and recipient bacteria, and reduced stability of plasmids once in the recipient bacteria [ 96 , 97 ].…”

Section: Potential Strategy To Improve the Efficiency Of Bioaugmenmentioning

confidence: 99%

Bioaugmentation: An Emerging Strategy of Industrial Wastewater Treatment for Reuse and Discharge

Nzila

¹

,

Razzak

²

,

Zhu

³

2016

IJERPH

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A promising long-term and sustainable solution to the growing scarcity of water worldwide is to recycle and reuse wastewater. In wastewater treatment plants, the biodegradation of contaminants or pollutants by harnessing microorganisms present in activated sludge is one of the most important strategies to remove organic contaminants from wastewater. However, this approach has limitations because many pollutants are not efficiently eliminated. To counterbalance the limitations, bioaugmentation has been developed and consists of adding specific and efficient pollutant-biodegrading microorganisms into a microbial community in an effort to enhance the ability of this microbial community to biodegrade contaminants. This approach has been tested for wastewater cleaning with encouraging results, but failure has also been reported, especially during scale-up. In this review, work on the bioaugmentation in the context of removal of important pollutants from industrial wastewater is summarized, with an emphasis on recalcitrant compounds, and strategies that can be used to improve the efficiency of bioaugmentation are also discussed. This review also initiates a discussion regarding new research areas, such as nanotechnology and quorum sensing, that should be investigated to improve the efficiency of wastewater bioaugmentation.

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“…Horizontal gene transfer is a tool for bioaugmentation. The introduced plasmids are transferred to indigenous bacteria via horizontal gene transfer like conjugation which plays a major role in spreading genetic information encoded on plasmids and can be exploited in bioaugmentation (Tsutsui et al 2010)..Plasmid transfer via conjugation has been frequently detected in many environments, including soils, activated sludge, sediments, the rhizosphere and phyllosphere of plants, and model, engineered and natural biofilms (Hausner et al 2011).…”

Section: The Role Of Catabolic Plasmid Transfer In Bioremediationmentioning

confidence: 99%

Transfer of the Catabolic Plasmid pJP4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells

Shamsad¹

2023

Preprint

0

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Limited information is available on how external environmental factors (e.g., the type of carbon source) affect biofilm architecture, conjugative transfer of the plasmid pJP4 and xenobiotic degradation in biofilms. The aim of this project was to investigate the influence of glucose and 2,4-dichlorophenoxyacetic acid, two different carbon sources which represent the absence and presence of selective pressure, respectively, on the combined effect of biofilm architecture, transfer of the plasmid pJP4 in soil derived mixed culture biofilms and consequent biodegradation of 2,4-D. The pJP4 plasmid was transferred to soil-derived mixed culture recipients in plate mating experiments and isolated transconjugant colonies were characterized as Comamonas testosteroni. Donor and transconjugant cells were not detected microscopically in biofilms and no transconjugant colonies were isolated; however, gfp, dsRed, and tfdB genes were detected in biofilm effluents with and without selective pressure. Heterogeneous biofilm architecture was observed for both with and without selective pressure.

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“…Horizontal gene transfer is a tool for bioaugmentation. The introduced plasmids are transferred to indigenous bacteria via horizontal gene transfer like conjugation which plays a major role in spreading genetic information encoded on plasmids and can be exploited in bioaugmentation (Tsutsui et al 2010)..Plasmid transfer via conjugation has been frequently detected in many environments, including soils, activated sludge, sediments, the rhizosphere and phyllosphere of plants, and model, engineered and natural biofilms (Hausner et al 2011).…”

Section: The Role Of Catabolic Plasmid Transfer In Bioremediationmentioning

confidence: 99%

Transfer of the Catabolic Plasmid pJP4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells

Shamsad¹

2023

Preprint

0

View full text Add to dashboard Cite

Limited information is available on how external environmental factors (e.g., the type of carbon source) affect biofilm architecture, conjugative transfer of the plasmid pJP4 and xenobiotic degradation in biofilms. The aim of this project was to investigate the influence of glucose and 2,4-dichlorophenoxyacetic acid, two different carbon sources which represent the absence and presence of selective pressure, respectively, on the combined effect of biofilm architecture, transfer of the plasmid pJP4 in soil derived mixed culture biofilms and consequent biodegradation of 2,4-D. The pJP4 plasmid was transferred to soil-derived mixed culture recipients in plate mating experiments and isolated transconjugant colonies were characterized as Comamonas testosteroni. Donor and transconjugant cells were not detected microscopically in biofilms and no transconjugant colonies were isolated; however, gfp, dsRed, and tfdB genes were detected in biofilm effluents with and without selective pressure. Heterogeneous biofilm architecture was observed for both with and without selective pressure.

show abstract

Bioremediation: Plasmid-Mediated Bioaugmentation of Microbial Communities – Experience from Laboratory-Scale Bioreactors

Cited by 4 publications

References 61 publications

Bioaugmentation: An Emerging Strategy of Industrial Wastewater Treatment for Reuse and Discharge

Bioaugmentation: An Emerging Strategy of Industrial Wastewater Treatment for Reuse and Discharge

Transfer of the Catabolic Plasmid pJP4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells

Transfer of the Catabolic Plasmid pJP4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells

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