Bioremediation potential of a highly mercury resistant bacterial strain Sphingobium SA2 isolated from contaminated soil

Mahbub, Khandaker Rayhan; Krishnan, Kannan; Megharaj, Mallavarapu; Naidu, Ravi

doi:10.1016/j.chemosphere.2015.08.061

Cited by 85 publications

(45 citation statements)

References 33 publications

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“…The crystalline structures of deposited Hg were visualized by SEM. Sathyavathi et al (2013) and Mahbub et al (2016) have previously reported the SEM analysis of same crystalline structures of detoxified Hg.…”

Section: Discussionmentioning

confidence: 90%

Cloning, Expression, Isotope Labeling, and Purification of Transmembrane Protein MerF from Mercury Resistant Enterobacter sp. AZ-15 for NMR Studies

Amin

Latif

2017

Front. Microbiol.

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Mercury resistant (HgR) Enterobacter sp. AZ-15 was isolated from heavy metal polluted industrial wastewater samples near to districts Kasur and Sheikhupura, Pakistan. 16S rDNA ribotyping and phylogentic analysis showed 98% homology with already reported Enterobacter species. The merF gene encoding transmembrane protein-MerF was amplified from genomic DNA and ligated into pET31b+ vector using restriction endonucleases, SphI and XhoI. The genetic codons of merF gene encoding cysteine residues were mutated into codons, translating into serine residues by site-directed mutagenesis. Ketosteroid isomerase (KSI), a fusion tag which is present in pET31b+ vector, was used in the expression of merFm gene. KSI was used to drive the target peptide (MerFm) into inclusion bodies so that the peptide yield and purity were increased. The stable plasmid pET31b+:merFm was transformed into C43(DE3) E.coli cells. The high expression of uniformly 15N isotopically labeled-MerFm protein was induced with 1 mM IPTG. The purification of 15N-MerFm recombinant protein by Ni-NTA and size exclusion chromatography involved an unfolding/refolding procedure. The two-dimensional HSQC NMR spectra of MerFm protein showed the purity and correct number of resonances for each amide. 1H–15N HSQC NMR experiment also confirmed that no modification of the tryptophan residue occurred during cyanogen bromide cleavage. A small scale reservoir of Luria Bertani (LB) medium supplemented with 20 μg/ml of HgCl2 showed 90% detoxification of Hg by Enterobacter sp. AZ-15. The accumulation of Hg on the cell surface of this strain was visualized by scanning electron microscopy (SEM) which confirmed its potential use in Hg-bioremediation.

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Section: Discussionmentioning

confidence: 90%

Cloning, Expression, Isotope Labeling, and Purification of Transmembrane Protein MerF from Mercury Resistant Enterobacter sp. AZ-15 for NMR Studies

Amin

Latif

2017

Front. Microbiol.

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“…De acuerdo al comportamiento observado, se evidencia un aumento en el crecimiento de microorganismos en suelo tratado, estos efectos se deben a la simbiosis que se produce entre las lombrices y los microorganismos del medio, tanto los que estaban en el suelo cuando se inició el proceso como las nuevas colonias registradas, evidenciándose una interacción lombrices-microorganismos [18,39].…”

Section: Resultados Y Discusiónunclassified

Efectos de la lombriz roja californiana (Eisenia foetida), sobre el crecimiento de microorganismos en suelos contaminados con mercurio de Segovia, Antoquia

Zapata

Martínez

Posada

et al. 2017

Cien.Ing.Neogranadina

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<p>Dentro de las actividades que alteran la composición del suelo está la minería, que causa el aumento de daños visibles al suelo mediante la creación de minas a cielo abierto, escombreras, estanques de residuos y otras instalaciones de minas. El objetivo principal de este trabajo fue evaluar el efecto de la lombriz roja californiana sobre el crecimiento de microorganismos en suelos contaminados con mercurio en el municipio de Segovia, Antioquia. Estos suelos representan una problemática importante debido a la acumulación de mercurio en exceso que ocasiona la pérdida de la calidad del suelo. Para este estudio se tomaron cuatro tratamientos en diferentes concentraciones de mercurio y un tratamiento más como blanco con lombrices rojas californianas <em>(Eisenia foetida). </em>Para esto, se evaluó el crecimiento de microorganismos a través de diferentes cultivos en el laboratorio, encontrando un crecimiento satisfactorio de los mismos luego de 25 y 90 días de tratamiento. De acuerdo al comportamiento observado, se evidencia una inmovilización del mercurio y una adaptación de los microorganismos al suelo contaminado, mostrando un aumento significativo de estos con el transcurso del tiempo.</p>

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“…Minimal inhibitory concentration (MIC) for growth was measured for seven of the most tolerant strains in microtiter plates containing YNBG broth. Strains selected were Cryprococcus agrionensis CRUB 1310 T , Cryptococcus sp.…”

Section: Methodsmentioning

confidence: 99%

Heavy metal capture by autochthonous yeasts from a volcanic influenced environment of Patagonia

Russo

Libkind

Giraudo

et al. 2016

J. Basic Microbiol.

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Heavy metals at elevated concentrations are a major threat to agricultural and human health. Typically, human activities tend to release these metals to the environment in aqueous solutions, generating high levels of pollution due to the mobility of the heavy metals. The aim of the present work was to assess heavy metal tolerance in yeasts isolated from Río Agrio - Lake Caviahue volcanic acidic aquatic environment and to evaluate the capacity of selected strains to capture metals in acidic culture media conditions. The ability of three yeast species, Cryptococcus agrionensis, Cryptococcus sp. 2, and Coniochaeta fodinicola, to tolerate and capture metals in live cultures has been evaluated. These three yeast species showed high tolerance to low pH and elevated concentrations of metals, thus implying their autochthonous status. Minimal inhibitory concentration (MIC) for growth obtained for these isolates showed elevated tolerance to the six heavy metals evaluated and were significantly higher than those registered for other microorganisms. C. agrionensis was able to capture 15.80 mg (g biomass) of Cu (MIC: 0.22 g L ), Cryptococcus sp. 2 was able to capture 36.25 and 65.28 mg (g biomass) of Ni and Zn , respectively (MIC: 0.56 and 1.68, respectively), and C. fodinicola was able to capture 67.11 mg (g biomass) of Zn (MIC: 3.75). This work reported the ability of yeasts to capture metals in acidic conditions for the first time. We hope that it represents the step-stone for future researches in the ability and metabolism of yeasts form acidic aquatic environment related to metal tolerance and capture.

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Bioremediation potential of a highly mercury resistant bacterial strain Sphingobium SA2 isolated from contaminated soil

Cited by 85 publications

References 33 publications

Cloning, Expression, Isotope Labeling, and Purification of Transmembrane Protein MerF from Mercury Resistant Enterobacter sp. AZ-15 for NMR Studies

Cloning, Expression, Isotope Labeling, and Purification of Transmembrane Protein MerF from Mercury Resistant Enterobacter sp. AZ-15 for NMR Studies

Efectos de la lombriz roja californiana (Eisenia foetida), sobre el crecimiento de microorganismos en suelos contaminados con mercurio de Segovia, Antoquia

Heavy metal capture by autochthonous yeasts from a volcanic influenced environment of Patagonia

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